江汉平原水稻白叶枯病菌致病型的变异研究

 从水稻和稻田周围常见的10种杂草上来集梨孢属菌株,以涂沫法作交互接种,分离自牛筋草、狗尾草、法氏狗尾草、李氏禾、罗氏草、铺地黍、稗和马唐的梨孢菌与分离自水稻的梨孢菌可以交叉侵染其寄主,茭白和碎米莎草梨孢菌与稻梨孢菌不能交互侵染;自然诱发条件下来自牛筋草和狗尾草的梨孢菌与来自水稻的梨孢菌对其寄主同样发生交叉侵染。直接从杂草寄主上分离的梨孢菌株对水稻的致病性较弱,接种到水稻感病品种发病后再分离的菌株,对水稻致病性增强,对原寄主的致病性基本保持不变。推断稻瘟病区稻田周围牛筋草、狗尾草、法氏狗尾草、李氏禾、罗氏草、铺地黍、稗和马唐等杂草寄主上的梨孢菌在稻瘟病菌的积累和传播方面具有作用。     

金龟子绿僵菌CQMa421对棉蚜、绿盲蝽的田间防效

棉花是我国重要的经济作物, 虫害严重制约了棉花生产, 造成棉农的经济损失。目前, 化学杀虫剂仍是防治棉花虫害的主要手段, 长期使用化学杀虫剂导致棉蚜Aphis gossypii、绿盲蝽Apolygus lucorum产生抗药性, 降低防治效果。本研究使用80亿孢子/mL金龟子绿僵菌CQMa421可分散油悬浮剂防治棉蚜、绿盲蝽, 室内生测结果表明, 金龟子绿僵菌CQMa421能有效侵染棉蚜和绿盲蝽, 随着浓度的升高, 对棉蚜和绿盲蝽的毒力增强。〖JP+2〗田间试验结果表明, 该药剂能有效控制棉花主要害虫棉蚜和绿盲蝽。采用1 200 mL/hm2用量, 药后14 d对棉蚜的防效达94.42%; 采用1 350 mL/hm2用量, 药后14 d对绿盲蝽的防效分别达到87.25%(新疆), 91.62%(湖北)。     

鳜鱼和奥利亚罗非血乳酸脱氢酶酶谱的比较

哺乳类动物各种组织和器官的乳酸脱氢酶(LDH)同工酶较稳定,酶带分布非常有规律,同源组织的LDH同工酶酶谱通常相似和稳定。鱼类LDH同工酶复杂得多,有三分之二品种中,其LDH同工酶类似哺乳类动物的酶谱;但在其余三分之一种类中,两个亚基随机结合受到限制,出现了不规则酶谱,有的只有2—3条酶带,有的超过5条酶带。即使具有5条酶带的鱼中,其骨骼肌和心肌LDH在电泳酶谱上迁移快慢正好与哺乳类动物相反。此外鱼类C基因产物并不特定在某一组织或器官中,一些鱼的     

浙江省滨海平原土壤中微量元素随利用时间的演变研究

为了解滨海平原耕地土壤中微量元素的变化特征,在浙北和浙南分别建立不同围垦利用时间的土壤系列,采集不同围垦利用时间的表层土壤混合样和剖面分层土样,分析土壤基本性状和微量元素全量与有效态含量。结果表明：围垦利用时间对微量元素有很大的影响,随着围垦利用时间的增加,土壤中铜、锌含量呈现增加,而铁、锰、硼、钼含量呈现下降;有效态铜、锌、铁、锰都呈现明显的增加,而有效态硼、钼含量呈现明显的下降;围垦时间的增长也增加了上下层土壤之间微量元素含量的差异,土壤剖面由上至下铜、锌和钼含量呈现下降,而铁、锰和硼呈现出向中下层增加的变化。围垦利用时间对微量元素的影响主要是土壤基本性状发生变化、长期种植水稻发生淋溶作用等综合作用的结果。     

杀虫真菌对草地贪夜蛾不同虫态的室内活性

草地贪夜蛾Spodoptera frugiperda（J.E.Smith）是一种联合国粮农组织预警的全球重大害虫。为了评价杀虫真菌对草地贪夜蛾的生防潜力,在室内条件下测定了杀虫真菌金龟子绿僵菌CQMa421、球孢白僵菌ZJU435对草地贪夜蛾不同龄期幼虫、蛹、成虫和卵的杀虫活性以及对卵孵化率、蛹羽化率等影响。结果显示：26℃条件下,菌株CQMa421和ZJU435对草地贪夜蛾低龄幼虫具有杀虫活性,接种10 d后对1龄幼虫的校正死亡率分别为35.6%和50.5%,但随虫龄增大杀虫活性降低,其中菌株CQMa421对4龄幼虫几乎没有杀虫活性;菌株CQMa421和ZJU435都引起草地贪夜蛾蛹黑化,降低其羽化率50%以上;但对成虫没有杀虫活性,也不影响成虫的产卵;卵接种菌株CQMa421和ZJU435后,孵化率分别为82.8%和90.7%,显著低于空白对照的100%,并极大地降低新孵化幼虫的存活率,分别为20.8%和0.0%,而空白对照为79.4%。结果表明,杀虫真菌CQMa421和ZJU435对草地贪夜蛾低龄幼虫、蛹和卵具有杀虫活性,同时降低卵的孵化率、新孵化幼虫存活率和蛹羽化率,对草地贪夜蛾种群控制极具潜力。     

Influence of in-house produced biochars on cracks and retained water during drying-wetting cycles: comparison between conventional plant,animal, and nano-biochars

Purpose

Biochars produced from different feedstocks (such as wood, pig manure) possess varying physical and chemical properties, which have influence on crack and evaporation rate of biochar-amended soil (BAS). Furthermore, influence of compaction state and drying-wetting cycles on evaporation rate and cracking of BAS has not been investigated comprehensively. The objective of this study was to investigate the effects of biochar types, compaction state of BAS, and drying-wetting cycles on crack propagation and retained water (or evaporation rate).

Material and methods

An animal and plant feedstock-based biochars were produced in-house from pig manure (PM) and wood (W), respectively. In addition, nano structured chalk and wheat biochar (CWB) were also produced. Soil amended with individual biochars was compacted in petri-glass discs at two densities. Disc specimens were subjected to multiple drying-wetting cycles, and evaporation rate of specimens and crack area were monitored throughout the experimental period (70 days). Images were captured after every 24 h and processed using image processing technique to obtain the crack intensity factor (CIF).

Results and discussion

The results show that plant-based W BAS showed the high water retention, i.e., low evaporation rate and low CIF. Furthermore, the crack potential of CW BAS was seen to be higher. In dense compacted soil, maximum CIF% can be reduced from 3.9 to 0.4% for W BAS, from 3.9 to 1.7% for PM BAS, and from 3.9 to 1.6% for CW BAS.

Conclusion

WB was able to resist cracking more efficiently than other types of biochar. Evaporation was found to be minimal for plant-based W BAS at 10% biochar percentage. Higher biochar content in soil was seen to increase the water retention of BAS significantly. Dense state of BAS at high biochar content (i.e., 10%) was effective in reducing evaporation rate and crack progression.

     

规模化养殖场畜禽粪中磷的化学形态与稳定化

为了解规模化养殖场畜禽粪便中磷的化学形态,寻求畜禽粪便中磷的稳定化途径,采集浙江省代表性规模化养殖场畜禽粪便样92个,采用改进的Hedley磷分级方法鉴定磷的化学形态;通过添加明矾、碳酸钙、石膏、粉煤灰及土壤物质,研究不同添加物对畜禽粪便中磷的稳定效果.结果表明:规模化养殖场畜禽粪便样品具有高磷、高无机磷比例及高生物有效性的特点,全磷质量分数在18.70~54.30 g/kg之间,平均为30.50 g/kg;可提取态无机磷占全磷的比例平均达70.08%;水溶性磷、Na HCO3-P和HCl-P占全磷的比例平均分别达20.03%、29.59%和22.93%,而Na OH-P和残余态磷占全磷的比例分别只有14.64%和12.81%.培养试验表明:各类稳定剂均对畜禽粪便中的磷有稳定效果,并随添加物用量的增加而增加;稳定效果为明矾粉煤灰碳酸钙土壤物质石膏.建议畜禽粪的施用量应基于其磷素水平确定,并适当添加稳定剂增加磷的稳定性.     

高速钻削复合材料的切削力

采用多种硬质合金钻头,在高转速和不同进给速度条件下对碳纤维/环氧树脂复合材料进行孔加工正交试验,通过研究其轴向力和扭矩以分析刀具条件和切削参数的适应性.结果表明:主轴转速是影响轴向力和扭矩的最主要因素,高转速钻削时轴向力和扭矩都显著减小;减小进给速度也使轴向力和扭矩减小,但其影响小于主轴转速.此外,钻头材质对轴向力和扭矩的影响大于钻头几何形状和进给速度;进给速度对轴向力的影响较大,而钻头几何形状对扭矩的影响较大.     

Long-term field evaluation and large-scale application of a Metarhizium anisopliae strain for controlling major rice pests

Journal of Pest Science - There is increasing concern about the toxic effects of chemical pesticides on human health and the environment. Many alternatives, however, are viewed as impracticable or...     

草鱼LDH同工酶比较酶学和免疫化学性质

本文以草鱼为材料,利用亲和层析法,从肌肉、心脏和肝脏中分离纯化了 LDH同工酶基因产物;LDH-A_4、B_4和C_4,并对其纯度,氨基酸组成以及动力学性质进行了比较分析,同时制取了A_4、B_4和C_4的相应抗体,利用抗原-抗体免疫吸附反应对一些淡水鱼类的LDH 酶谱进行了准确的鉴定。