Optimal feed level during the transition period to achieve faster farrowing and high colostrum yield in sows

This study aimed to determine the optimal supply of lactation feed during the transition period to minimize farrowing duration (FD) and maximize colostrum yield (CY) and quality with the overall aim of reducing piglet mortality. A total of 48 sows were stratified for body weight and assigned to six levels of feed supply (1.8, 2.4, 3.1, 3.7, 4.3, and 5.0 kg/d) from day 108 of gestation until 24 h after the onset of farrowing. The number of total born, live-born, and stillborn piglets; birth time and birth weight of each piglet; and frequency of farrowing assistance (FA) was recorded, and blood samples were obtained from newborn piglets at birth. Live-born piglets were further weighed at 12 and 24 h after birth to record weight gain, which in turn was used to estimate intake and yield of colostrum. Colostrum samples were collected at 0, 12, 24, and 36 h after the onset of farrowing. FD was shortest (4.2 h) at intermediate (3.7 kg/d), longest (7.1 to 7.6 h) at low (1.8 and 2.4 kg/d), and intermediate (5.6 to 5.7 h) at high (4.3 and 5.0 kg/d) feed intake (P = 0.004; mean comparison). FA was lowest (0.7% to 0.8%) at intermediate feed intake (3.7 and 4.3 kg/d) and substantially elevated (4.3% to 4.7%) at both lower and higher feed intake (P = 0.01; mean comparison). The cubic contrast revealed 4.1 kg/d as the optimal feed intake to achieve the shortest FD and to minimize FA. Newborn piglets from second-parity sows were less vital than piglets from gilts as evaluated by blood biochemical variables immediately after birth. CY was greatest at 3.1 kg/d (P = 0.04), whereas the cubic contrast revealed 3.0 kg/d as the optimal feed intake to maximize CY. Concentrations of colostral components were affected by the diet, parity, and their interaction except for lactose concentrations. In conclusion, the study demonstrated the importance of proper feed level during the transition period on sow productivity. Moreover, this study estimated 4.1 and 3.0 kg/d as the optimal feed intake during the transition period to improve farrowing characteristic and CY, respectively, and these two feed intake levels supplied daily 38.8 MJ metabolizable energy (ME) and 23.9 g standardized ileal digestible (SID) lysine (3.0 kg/d) or 53.0 MJ ME and 32.7 g SID lysine (4.1 kg/d). The discrepancy of optimal feed intake for optimal farrowing and colostrum performance suggests that it may be advantageous to lower dietary lysine concentration in the diet fed prepartum.     

High molecular weight glutenin subunits of current bread wheats grown in Iran

Variation of high molecular weight gluteninsubunits (HMW-GS) at the Glu-1 lociwas studied using SDS-PAGE method in 43advanced lines or cultivars commonly grownin Iran. Fourteen alleles and 21 alleliccompositions were detected. Among the 43bread wheats analysed only five showed aunique HMW-GS composition. The mostfrequent HMW-GS patterns were 2^*, 7+8,2+12 and 2^*, 17+18, 2+12 which wereobserved in 13 and six cultivars,respectively. In the remainings, each twoto three lines or cultivars showed a commonHMW-GS pattern. Therefore, allelicvariation at Glu-1 loci is of limitedvalue for cultivar identification comparedwith loci controlling gliadins. Sevencultivars were observed to consist of twoto three biotypes with different alleles.In cultivar Mahdavi a biotype showedinactivity at the Glu-B1 locus. Analready reported rare subunit pair'2^***+12' at Glu-D1locus was found in cultivar Kavir. Theresults of scoring cultivars for theirquality based on the HMW-GS compositionswith an average score of eight, wasgenerally good. Cultivars Inia, Tajan, andadvanced lines N-75-11 and N-75-15 showedquality score equal to 10, whereas Alamootand C-75-5 showed quality scores equal tofive. The quality of former and latterlines and cultivars were considered highestand lowest, respectively. The resultsobtained in this study are useful inbreeding programs to improve bread makingquality, developing uniformity andimproving heterogeneous cultivars by meansof selection of the best genotypes.     

Elevation, rootstock, and soil depth affect the nutritional quality of mandarin oranges

The effects of elevation, rootstock, and soil depth on the nutritional quality of mandarin oranges from 11 groves in California were investigated by nuclear magnetic resonance (NMR) spectroscopy by quantifying 29 compounds and applying multivariate statistical data analysis. A comparison of the juice from oranges in groves with deeper soil and trifoliate rootstock versus those with shallow soil and C-35 rootstock revealed differences in the concentrations of 4-aminobutyrate, ethanol, phenylalanine, succinate, and isoleucine. A comparison of fruit from trees grown at higher versus lower elevation revealed that those at higher elevation had higher concentrations of amino acids, succinate, and 4-aminobutyrate and lower concentrations of sugars and limonin glucoside. Such differences indicate that rootstock, soil depth, and differences in elevation influence the fruit nutrient composition. This study highlights how metabolomics coupled with multivariate statistical analysis can illuminate the metabolic characteristics of citrus, thereby aiding in the determination of the grove identity and fruit quality during orange production.     

Bioremediation of Hexachlorocyclohexane-Contaminated Soil by the New <Emphasis Type="Italic">Rhodococcus wratislaviensis</Emphasis> Strain Ch628

Currently, there are large areas of soils contaminated with hexachlorocyclohexane (HCH) isomers which are included in the group of persistent organic pollutants. For the bioremediation of such soils, a new HCH-degrading Rhodococcus wratislaviensis strain Ch628 was isolated from long-term organochlorine contaminated soils. The strain Ch628 was able to degrade 32.3% γ-hexachlorocyclohexane (γ-HCH/lindane), 25.2% hexachlorobenzene, and 100% chlorobenzene in resting cell conditions. The strain Ch628 was bioaugmented in chronically HCH-contaminated soil. The results showed that the bioaugmentation of contaminated soil with the strain Ch628 led to HCH degradation. In the bioaugmented system, the efficiency of HCH removal at the initial concentration of about 238.7 ± 4.9 mg kg^?1 soil was 44.8%, while the system with indigenous microflora (without R. wratislaviensis strain Ch628) and the system with abiotic control removed 33.3 and 16.4% of this compound during the same period, respectively. Strain Ch628 could effectively degrade α-, β-, and γ-isomers of HCH (77.1, 100, and 100%, respectively) and heptachlorocyclohexane (69.9%) in the model soil systems. Moreover, the bioaugmentation with the strain Ch628 led to degradation of tri-, tetra-, and penta-chlorobenzenes, which are of HCH degradation metabolites. For the first time, it was found that the bioaugmentation with the bacterial strain Rhodococcus wratislaviensis Сh628 led to a significant reduction of the toxicity of the HCH-contaminated soil for the test organisms, such as Chlorella vulgaris Beijer and Daphnia magna Straus.     

Turbulent mixing accelerates PAH desorption due to fragmentation of sediment particle aggregates

Purpose

Stripping contaminants from sediments with granular activated carbon (GAC) is a promising remediation technique in which the effectiveness depends on the rate of contaminant extraction from the sediment by the GAC. The purpose of the present study was to investigate the effect of mixing intensity on the short-term extraction rate of polycyclic aromatic hydrocarbons (PAHs) from contaminated sediment.

Materials and methods

PAH desorption from sediment at a wide range of rotational speeds (min^?1; rotations per minute (rpm)) was monitored by uptake in Tenax polymeric resins using a completely mixed batch reactor. Desorption data were interpreted using a radial diffusion model. Desorption parameters obtained with the radial diffusion model were correlated with particle size measurements and interpreted mechanistically.

Results and discussion

Fast desorption rate constants, D _e /r ², with D _e the effective diffusion coefficient and r the particle radius, ranged from 3.7 × 10^?3 to 1.1 × 10^?1 day^?1 (PHE) and 6 × 10^?6 to 1.9 × 10^?4 day^?1 (CHR), respectively, and increased with the intensity of mixing. The D _e /r ² values would correspond to D _e ranges of 1.8 × 10^?14–1.2 × 10^?16 m² × day^?1 and 1.8 × 10^?12–3.7 × 10^?15 m² × day^?1, assuming fast desorption from the measured smallest particle size (9 μm) classes at 200 and 600 rpm, respectively.

Conclusions

Desorption of PAHs was significantly accelerated by a reduction of particle aggregate size caused by shear forces that were induced by mixing. The effective intra-particle diffusion coefficients, D _e , were larger at higher mixing rates.

     

The ancient drug salicylate directly activates AMP-activated protein kinase

Salicylate, a plant product, has been in medicinal use since ancient times. More recently, it has been replaced by synthetic derivatives such as aspirin and salsalate, both of which are rapidly broken down to salicylate in vivo. At concentrations reached in plasma after administration of salsalate or of aspirin at high doses, salicylate activates adenosine monophosphate-activated protein kinase (AMPK), a central regulator of cell growth and metabolism. Salicylate binds at the same site as the synthetic activator A-769662 to cause allosteric activation and inhibition of dephosphorylation of the activating phosphorylation site, threonine-172. In AMPK knockout mice, effects of salicylate to increase fat utilization and to lower plasma fatty acids in vivo were lost. Our results suggest that AMPK activation could explain some beneficial effects of salsalate and aspirin in humans.     

Effects of crude oil contamination on soils of the Ural region

Journal of Soils and Sediments - Nowadays, crude oil contamination of soil is one of the widespread environmental problems. In this paper, we examine the impact of oil contamination on individual...     

Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1

Herpes simplex virus type 1 (HSV-1) is one of the most prevalent pathogens worldwide requiring the search for new candidates for the creation of antiherpetic drugs. The ability of sea urchin spinochromes—echinochrome A (EchA) and its aminated analogues, echinamines A (EamA) and B (EamB)—to inhibit different stages of HSV-1 infection in Vero cells and to reduce the virus-induced production of reactive oxygen species (ROS) was studied. We found that spinochromes exhibited maximum antiviral activity when HSV-1 was pretreated with these compounds, which indicated the direct effect of spinochromes on HSV-1 particles. EamB and EamA both showed the highest virucidal activity by inhibiting the HSV-1 plaque formation, with a selectivity index (SI) of 80.6 and 50.3, respectively, and a reduction in HSV-1 attachment to cells (SI of 8.5 and 5.8, respectively). EamA and EamB considerably suppressed the early induction of ROS due to the virus infection. The ability of the tested compounds to directly bind to the surface glycoprotein, gD, of HSV-1 was established in silico. The dock score of EchA, EamA, and EamB was −4.75, −5.09, and −5.19 kcal/mol, respectively, which correlated with the SI of the virucidal action of these compounds and explained their ability to suppress the attachment and penetration of the virus into the cells.