Structure of the sheep epidermis.

The stratum corneum measured on fresh frozen sections was found to be considerably thicker than previously reported. It was uniformly permeated with sebum, which also formed an intact film over the skin surface and it could readily be divided into two zones. The living epidermis was approximately half as thick as the stratum corneum.     

Effect of foliar-applied potassium chloride on septoria leaf blotch of winter wheat

The effect of foliar-applied potassium chloride on Septoria tritici , the anamorph of Mycosphaerella graminicola , was quantified and possible modes of action investigated during controlled-environment and field experiments. A field experiment in harvest year 1997 showed c . 50% reduction in the area of leaf 2 of winter wheat plants affected by septoria leaf blotch after foliar application of potassium chloride, compared with untreated controls. Similarly, in harvest year 1998 potassium chloride reduced, by about one-third, the area of the flag and penultimate leaf affected by S. tritici . However, a significant yield increase was not observed, although grains m⁻² did show an increase of borderline significance. Applications of epoxiconazole reduced the area of leaf 4 affected by S. tritici compared with untreated controls, whereas applications of chlorothalonil, potassium chloride or polyethylene glycol proved ineffective against disease development. This may suggest that potassium chloride is relatively immobile and possesses contact activity similar to that of chlorothalonil. In 1998, similar reductions in leaf area affected were observed with the inert osmoticum polyethylene glycol in the field, suggesting that the control provided by potassium chloride may be achieved by adverse osmotic effects on the pathogen. Scanning electron microscopy of germinating conidia on wheat plants showed inhibition of conidial germination by both potassium chloride and polyethylene glycol at the same calculated osmotic potential on the leaf surface.     

A survey of northern Victorian dairy farmers to investigate dairy calf management: colostrum feeding and management

Objectives

To describe colostrum management practices carried out in northern Victorian dairy herds and to identify weaknesses in these areas that may affect calf health and welfare by comparing the results with the current industry recommendations

Methods

A questionnaire to obtain information about colostrum management and calf‐rearing practices was sent to commercial dairy farming clients of Rochester Veterinary Practice between June and September 2013. The questionnaire consisted of a general herd overview and colostrum harvesting practices.

Results

The response rate was 39% (58/150). Many dairy producers were not meeting the current industry recommendations in the following areas: (1) time of removal calf from the dam, (2) relying on calf suckling colostrum from the dam to achieve adequate passive transfer, (3) failing to supplement calves with colostrum, (4) feeding inadequate volumes of colostrum, (5) delayed colostrum harvesting, (6) pooling of colostrum, (7) failing to objectively assess colostrum quality or relying on visual assessment and (8) storing colostrum for a prolonged periods of time at ambient temperatures.

Conclusion

The results from this survey highlight the need for greater awareness of industry standards for colostrum management and feeding hygiene.

     

An introduction to the global nitrogen cycle

Abstract. Current estimates are tabulated for the quantities of nitrogen circulating in the global nitrogen cycle. Five gases, NH₃, N₂O, NO, NO₂ and N₂, dominate the movement of nitrogen between the earth's surface and the atmosphere. The input of combined nitrogen to the land surface of the earth is tentatively estimated at 290 million tonnes per year, a total which includes 74 million tonnes from fertilizers. Known outputs from land (as gaseous NH₃, N₂O and NO_X, and as inorganic nitrogen carried to the sea by rivers) are much less, totalling 130 million tonnes per year. Emissions of N₂ gas probably account for most of the difference. There has been an increase in the use of nitrogen of about 5% per year over the last ten years. The demand for fertilizer nitrogen is likely to continue to grow if the population of the world continues to increase.     

The effects of biocidal treatments on metabolism in soil—III. The relationship between soil biovolume,measured by optical microscopy,and the flush of decomposition caused by fumigation

The hypothesis that the flush of decomposition following fumigation is a measure of the amount of biomass in a soil was tested by comparing the biomass thus found, and as calculated from direct microscopic measurement of the soil biovolume. Eight soils developed under contrasting systems of management and climate were used, six from England and two from Nigeria. The biovolume was measured by a modification of the Jones and Mollison procedure, in which agar films prepared from known amounts of soil were stained with phenolic aniline blue. Fluorescent stains were less satisfactory in that they did not stain as wide a range of organisms as phenolic aniline blue. Spherical organisms were divided into 13 size classes, with diameters ranging from 0·3 to 19 μm, and the numbers in each size class counted. Hyphae were divided into seven diameter classes, with dia. ranging from 1 to 11 μm, and the lengths in each class measured. For seven of the soils there was close agreement between the two different methods of measuring biomass C. With an acid woodland soil (pH 3·9). the biomass C, as calculated by direct microscopy, was seven times that calculated from the size of the flush; it is suggested that this discrepancy arose because stainable cell walls from dead organisms persist for much longer in the strongly acid soil than in the other, more nearly neutral, soils.Both methods were used to follow the effects of fumigation on the soil biomass. A near-neutral soil was fumigated with CHCl₃, the fumigant removed and the soil incubated for 53 days at 25° C. The decrease in biomass caused by fumigation was less as measured by direct microscopy than as measured from the size of the flush, suggesting that stainable cell walls of killed organisms can persist for a considerable time after fumigation.Data on the relationship between the size of soil organisms and their contribution to soil biovolume are presented graphically. The biovolume in hyphae and in ‘spherical’ organisms was roughly equal in all soils. There was a linear relationship between the cumulative biovolume and the logarithm of organism volume lor ‘spherical’ organisms over the volume range 0·05 μm³ to 100 μm³. If, for a given soil, the volume range is divided into equal volume classes on a logarithmic basis, each class contains the same biovolume. Thus, comparing equal logarithmic volume classes, a class of rare large organisms contains as much biovolume as a class of numerous small organisms.     

A method for measuring adenosine triphosphate in soil

A method was devised for the extraction and measurement of adenosine 5'-triphosphate (ATP) in soil that minimizes sorption of ATP on the soil colloids. Soil was ultrasonified for 1 min with a solution containing trichloracetic acid (0.5 m). disodium hydrogen orthophosphate (0.25 m) and paraquat dichloride (0.1 m). The ATP content of the filtered extract was determined without further treatment in a scintillation spectrometer by the firefly luciferin-luciferase system. Recovery of added ATP was greater using the extratant containing trichloracetic acid, orthophosphate and paraquat than with trichloracetic acid alone or with a sulphuric acid extradant. Recoveries of added ATP ranged from 45% to 84% in thirteen different soils; ATP contents from 0.64 to 9.03 μg g^?1 soil.