Rupture of the Vinculum in Association with Tendon Injury within the Digital Flexor Tendon Sheath in Two Horses

The following report documents an unusual anatomical finding in two cases of aseptic tenosynovitis of the digital flexor tendon sheath. Disruption of the vinculum attachment between the palmar/plantar border of the superficial digital flexor tendon and the adjacent palmar/plantar annular ligament was identified in combination with other tendon pathology within the digital flexor tendon sheath. This tenoscopic finding has not been described previously in the literature.     

Investigating Neolithic land use in Swifterbant (NL) using micromorphological techniques

In the Swifterbant area in The Netherlands, a complete Neolithic landscape is preserved, buried in a wetland environment. A dozen sites (dating from ca. 4300–4000 cal. BC) on levees of a former creek system are characterized by a black layer containing large amounts of carbonized plant remains, burnt bone, flint and pottery. These sites are usually interpreted as occupation sites with accumulated refuse of a society in transition from a Mesolithic to a Neolithic lifestyle (hunting and herding), in an area that was too wet for crops.In the context of a new research campaign in the area, we investigated the site- and land-use on two locations (S2 and S4) using micromorphological techniques.On S2, the soil matrix in the archaeological horizon has a heterogeneous, non-sedimentary appearance due to the ubiquitous presence of rounded and subrounded aggregates. These aggregates indicate that the sediment was disturbed after deposition, but it is unclear by what human activity. This disturbance is not restricted to the archaeological site horizon, but extends also into the under- and overlying layers.On S4, three layers can be distinguished. Only the lower and central parts are usually regarded as archaeological layers. The lower layer shows thorough mixing of the (partly decalcified) groundmass and the incorporation of anthropogenic materials (carbonized plant remains, burnt bone, etc.). Most probably, the thorough mixing is a result of tillage. The central layer consists of thinly laminated phytoliths and carbonized plant remains with ample fragments of (burnt) bone, angular sediment clods and some dog coprolites. Mineral sediments are absent. Apparently, the layer was formed by human activities that resulted in a massive accumulation of burnt organic materials and anthropogenic debris. The preservation of the micro-layering formed by phytoliths and carbonized is remarkable, since trampling would have deformed or disturbed the layering. The surprising conclusion therefore has to be that this location was not the main settlement area—as was implicitly thought until now. Rather, the area should be interpreted as a location where specific activities resulted in the accumulation of burnt plant material, e.g. a waste dump. The upper layer again has indications of anthropogenic soil disturbance. Samples from the basin adjacent to the levee site show sediments that are deformed by trampling. The transition of the site to the channel shows no signs of trampling, but rather interfingering of site layers with channel sediments. Lack of trampling in the central zone of S4 and the adjacent channel shore is at odds with the classical interpretation that the site represents a settlement area. The black layers that up till now were thought to encompass a complete settlement, could very well be a very prominent part of larger sites with remains of human activities during the Neolithic.     

Antral Follicle Populations and Embryo Production – In Vitro and In Vivo – of Bos indicus–taurus Donors from Weaning to Yearling Ages

Interest in indicus–taurus cattle has been increasing, as these animals are likely to present the best characteristics of Zebu and European bovine breeds. The aim of this study was to compare the embryo production of indicus–taurus donors with high vs low antral follicle counts obtained by ovum pickup/in vitro production (OPU/IVP) and superovulation (SOV)/embryo collection. Braford females at weaning age (3/8 Nelore × 5/8 Hereford, n = 137, 9 ± 1 month old) were subjected to six serial ovarian ultrasonographs and were assigned to two groups according to the number of antral follicles ≥3 mm as follows: G‐High antral follicular count (AFC, n = 20, mean ≥40 follicles) and G‐Low AFC (n = 20, mean ≤10 follicles). When the females (n = 40) reached 24 months of age, they were subjected to both OPU/IVP and SOV/embryo collection. The average number of follicles remained highly stable throughout all of the ultrasound evaluations (range 0.90–0.92). The mean number of COCs recovered (36.90 ± 13.68 vs 5.80 ± 3.40) was higher (p < 0.05) for females with high AFC, resulting in higher (p < 0.05) numbers of total embryos among females with high vs low AFC (6.10 ± 4.51 vs 0.55 ± 0.83). The mean number of embryos per collection was also higher (p < 0.05) for G‐High vs G‐Low (6.95 ± 5.34 vs 1.9 ± 2.13). We conclude that a single ultrasound performed at pre‐pubertal ages to count antral follicles can be used as a predictor of embryo production following IVP and SOV/embryo collection in indicus–taurus females.     

Bluetongue sentinel surveillance program and cross-sectional serological survey in cattle in Belgium in 2010-2011

Bluetongue virus serotype 8 (BTV-8) emerged in Central Western Europe in 2006 causing a large scale epidemic in 2007 that involved several European Union (EU) countries including Belgium. As in several other EU member states, vaccination against BTV-8 with inactivated vaccines was initiated in Belgium in spring 2008 and appeared to be successful. Since 2009, no clinical cases of Bluetongue (BT) have been reported in Belgium and BTV-8 circulation seemed to have completely disappeared by spring 2010. Therefore, a series of repeated cross-sectional surveys, the BT sentinel surveillance program, based on virus detection in blood samples by means of real-time RT-PCR (RT-qPCR) were carried out in dairy cattle from the end of 2010 onwards with the aim to demonstrate the absence of BTV circulation in Belgium. This paper describes the results of the first two sampling rounds of this BT sentinel surveillance program carried out in October-November 2010 and January-February 2011. In addition, the level of BTV-specific maternal antibodies in young non-vaccinated animals was monitored and the level of herd immunity against BTV-8 after 3 consecutive years of compulsory BTV-8 vaccination was measured by ELISA. During the 1st sampling round of the BT sentinel surveillance program, 15 animals tested positive and 2 animals tested doubtful for BTV RNA by RT-qPCR. During the 2nd round, 17 animals tested positive and 5 animals tested doubtful. The positive/doubtful animals in both rounds were re-sampled 2-4 weeks after the original sampling and then all tested negative by RT-qPCR. These results demonstrate the absence of BTV circulation in Belgium in 2010 at a minimum expected prevalence of 2% and 95% confidence level. The study of the maternal antibodies in non-vaccinated animals showed that by the age of 7 months maternal antibodies against BTV had disappeared in most animals. The BTV seroprevalence at herd level after 3 years of compulsory BTV-8 vaccination was very high (97.4% [95% CI: 96.2-98.2]). The overall true within-herd BTV seroprevalence in 6-24 month old Belgian cattle in early 2011 was estimated at 73.4% (95% CI: 71.3-75.4).