Simultaneous determination of 20 pharmacologically active substances in cow's milk, goat's milk, and human breast milk by gas chromatography-mass spectrometry

This paper reports a systematic approach to the development of a method that combines continuous solid-phase extraction and gas chromatography-mass spectrometry for the simultaneous determination of 20 pharmacologically active substances including antibacterials (chloramphenicol, florfenicol, pyrimethamine, thiamphenicol), nonsteroideal anti-inflammatories (diclofenac, flunixin, ibuprofen, ketoprofen, naproxen, mefenamic acid, niflumic acid, phenylbutazone), antiseptic (triclosan), antiepileptic (carbamazepine), lipid regulator (clofibric acid), β-blockers (metoprolol, propranolol), and hormones (17α-ethinylestradiol, estrone, 17β-estradiol) in milk samples. The sample preparation procedure involves deproteination of the milk, followed by sample enrichment and cleanup by continuous solid-phase extraction. The proposed method provides a linear response over the range of 0.6-5000 ng/kg and features limits of detection from 0.2 to 1.2 ng/kg depending on the particular analyte. The method was successfully applied to the determination of pharmacologically active substance residues in food samples including whole, raw, half-skim, skim, and powdered milk from different sources (cow, goat, and human breast).     

Impact of Simulated Grazing on the Recovery,Production and Modular Dynamic of the White Wormwood Artemisia herba-alba Asso.

One of the species candidates to rehabilitate arid rangelands of Morocco is Artemisia herba-alba Asso. （white Wormwood）. Unfortunately, overgrazing is by far the most important factor causing the reduction of cover and disappearance of this plant. In order to assess the effect of season and grazing on the recovery ofA. herba-alba Asso., plants of similar size were collected from Gourrama region and transplanted in the Experimental Station of Errachidia. These plants were submitted to different clipping heights （Cl = 8 cm, C2 = 15 cm, C3 = 25 cm） during two growing periods： autumn （stage of seed filling） and spring （before the starting of flowering）. Mean results show highly significant （P 〈 0.01） differences between the three treatments during the two periods. The increase of the plant volume clipped influences the performances of production and regrowth, the study has shown that the cutting level C 1 affect negatively the Artemisia performances, in fact 60% of plants cut at this height have died after the second clipping against 25% for C2 and 4.2% for C3. The parameters of growth and modular dynamic of branches are also affected by the cutting height C 1. The phytomass produced after the first cutting results in highly significant differences （P 〈 0.0 l） between the three cutting heights. Finally, the study demonstrated that for better regrowth, the taller height C3 is the most convenient for white Wormwood during the two periods, but the cutting height C2 can also be adopted for the autumn period.