从害虫马尾松毛虫中提取甲壳素的初步研究

采用酸碱法对马尾松毛虫蛹中甲壳素的提取方法进行了初步研究。重点分析了N aOH不同处理条件对脱有机物质和甲壳素产率的影响,确定了从马尾松毛虫蛹中提取甲壳素的基本工艺:(1)脱矿物质:盐酸浓度为2.5%,浸泡时间为20 h,浸泡温度为30℃;(2)脱有机物质:N aOH浓度为6%,浸泡温度为75℃,浸泡时间为10 h;(3)脱色:双氧水浓度为9%,浸泡时间为2.5 h,浸泡温度为80℃。在此条件下,获得的甲壳素产品为白色粉状固体,其N含量为6.87%,灰分为1.19%,水分为8.37%。产率为29.97%。产品的N含量达到食品级甲壳素标准,灰分含量达到工业级甲壳素标准。实验有助于后续深入研究马尾松毛虫蛹中甲壳素的提取,也为今后进一步制备虫蛹壳聚糖打下了基础。     

Effect of a Single Dose of Cadmium on Pregnant Wistar Rats and their Offspring

Cadmium (Cd) is a well‐known toxicant targeting many organs, among them placenta. This heavy metal also has embryonary and foetal toxicity. This study was undertaken to analyse the effect of a single Cd dose administered at 4, 7, 10 or 15 days of gestation on the offspring of pregnant rats sacrificed at 20 days of gestation. Cadmium chloride was administered subcutaneously at 10 mg/kg body weight to Wistar pregnant dams; control animals received a proportionate volume of sterile normal saline by the same route. Maternal uteri, livers, kidneys and lungs, and foetuses were examined at necropsy. Samples of maternal organs and whole foetuses were collected for histopathologic examination, determination of Cd levels and staining by the Alizarin red S technique. Results revealed a clear embryotoxic and a teratogenic effect of this heavy metal, the former as a significant increase in the number of resorptions, and the latter as significant decrease of the gestational sac weight, and the size and weight of foetuses of Cd‐treated dams as well as induced malformations in skull bones, vertebrae and thoracic, and pelvian limbs. The deleterious effects found were similar to those previously reported for other animal models suggesting a high conservation of the pathogenic mechanisms of Cd. Additionally, many of the addressed aspects showed a slight dependence on the time of administration of the toxic that might be due to the accumulation of the metal in different organs, as we were able to demonstrate by the analysis of its concentration.     

The effect of water stress and rootstock on the development of leaf injuries in grapevines irrigated with saline effluent

Deficit irrigation is increasingly being practiced in water-limited areas to overcome water scarcities. Although, this practice reduces yield losses, there is limited information currently available on how this practice can affect crops when the irrigation water contains elevated levels of salts. An experiment was set up to investigate salt uptake and distribution and salt tolerance of potted Soultanina vines grafted on different rootstocks (41B, 1103P, 110R) and irrigated with effluent containing relatively high concentrations of salts and fresh water at different fractions of evapotranspiration (0.50, 0.75 and 1.00ET). Irrigation with recycled water induced the development of leaf burns, which were more intense in 1998 despite the lower leaf-Na and -Cl content. This may have been due to the more severe water deficit and/or to the more adverse climatic conditions which prevailed during that season. Decreasing the irrigation level induced the development of leaf burns causing only minor changes to leaf-Na or -Cl content. Differences in salt uptake, accumulation and distribution were observed among the rootstocks investigated in this work, suggesting that differences exist in the mechanisms regulating salt uptake and distribution in the shoot. Despite these differences, a distinct superiority in terms of salinity tolerance among rootstocks was only observed at the 0.50ET irrigation level, where vines grafted on 41B developed earlier and more acute leaf burns than the other rootstocks. These findings suggest that leaf salt content alone it is not to classify genotypes according to their tolerance to salinity and that salinity-induced damage is linked with prevailing environmental conditions. Furthermore, it can be inferred that grapevines have additional mechanisms to cope with salt stress which may counteract differences in salt uptake and accumulation in the shoot.