建昌黑山羊生产性能测定

2003年以来,开展了建昌黑山羊生产性能测定。经测定,成年公、母羊体重(kg)分别为38.40±6.44和35.49±4.73,成年公、母羊体高(cm)分别为64.00±2.27和61.57±4.04,成年公(羯)、母羊屠宰率分别为52.94%和48.36%,母羊产羔率为155.80%,经产母羊月均泌乳12.10kg。     

云南省稻瘟病,小麦锈病预测专家系统

基于稻瘟病、小麦锈病预测领域专家知识,以及借助模式识别势函数法实现的自学习、自提取的知识,采用正向和反向精确推理控制策略,首次建立云南省水稻穗瘟病、小麦条锈病预测专家系统,1994～1995年预测结果与实际情况基本一致。系统创造性地采用全屏幕编辑树图的知识输入法和森林表示技术对知识库进行管理,便于知识库的扩充和维护。推理结果、推理路径及知识查询以彩色图形方式显示,对于学习领域专家的实践经验具有独特优点。     

Effects of five cryoprotectants on proliferation and differentiation‐related gene expression of frozen‐thawed bovine calf testicular tissue

The cryopreservation of testicular tissue is a potential method for preserving male fertility. However, the effect of cryopreservation on bovine calf testicular tissue is scarce. This study investigated the effect of different cryoprotectants on bovine calf testicular tissue at the molecular level. Testicular tissue from ten immature bovine calves (6 months) was collected after slaughter and cryopreserved in an extender containing different concentrations of the following five cryopreservation solutions (CP): bovine serum albumin (BSA) with 5% dimethyl sulfoxide (DMSO), trehalose with 5% DMSO, DMSO and glycerol and ethylene glycol (EG). After 7‐day cryopreservation, the expression levels of three spermatogonial stem cell (SSC)‐related genes, octamer‐4 (OCT4), KIT ligand (MGF/SCF) and kit oncogene (C‐KIT), were investigated by quantitative PCR (qPCR). The cell viability was highest for the tissues preserved with 30 mg/ml BSA (77.82% ± 1.22) and 40 mg/ml trehalose (74.23% ± 1.16) compared with other groups (p < 0.05), and the level of expression of the three genes was highest with 30 mg/ml BSA (p < 0.05). Compared with other CPs, the 30 mg/ml BSA and 40 mg/ml trehalose have the better cryopreserve protection. The 30 mg/ml BSA is the most viable media for the cryopreservation of testicular tissue from cattle.     

自动车床工序调整卡的计算机辅助设计

“经验式”和“创成式”是计算机辅助工艺过程设计的两种基本的技术方法.后一种技术方法虽然还不够成熟,但它在CAPP系统的设计与实现上具有很多的优势.描述了计算机辅助设计在自动车床加工中的一些应用,它涉及到工艺方案的制定和加工工序的安排.另外,它描述了一些特有的计算机算法和编程原理.     

客货车ABS工作原理与检修

着重介绍了用压缩空气作介质的客货车ABS系统,介绍了车轮防抱死系统的结构、原理,对客货车ABS所使用的压缩空气制动系统,及其安装调试及诊断维修作了详细的描述,这对从事客货车维修的工作人员有一定的指导意义.     

Immunogenicity of multiple-epitope antigen gene of HCV carried by novel biodegradable polymers

In order to develop a promising vaccine candidate utilizing a combined approach to induce both antibody production and T-cell activity, the DNA fragment containing MA of HCV with five conserved epitopes was synthesized. Two types of HCV vaccine candidates (the DNA type and DNA/polymers) were constructed using MA. PLA-PEG-PLA and PLGA-PEG-PLGA were synthesized and used as micelles with encapsulated plasmid pcDNA3.1(+)-MA. The preparation of copolymers, the cloning and analysis of recombinant plasmid DNA, in vitro expression, and immunogenicity in transgenic mice were evaluated in detail. The results indicated that even single immunization and oral immunization with DNA/polymers achieved satisfying immune responses in vivo tests. As biodegradable and nontoxic triblock copolymers, the novel copolymers demonstrated a great advantage, as they made long-term and single-immunizing vaccines possible; in addition, the copolymers showed a better adjuvant effect and scarcely any side effects.     

基于Cortex-M3的农作物生长参数监测系统设计

为了快速、全面地获取农作物生长过程中的参数变化(土壤墒情、雨量、地下水位),满足农业生产对信息的需求,提高粮食产量,设计开发了基于Cortex-M3的农作物生长参数监测系统。系统用多种智能传感器组成传感器网络,能够实时采集雨量、土壤含水率和地下水位数据,经软件解析、处理后,通过GPRS网络实现数据的无线传输。系统供电采用太阳能电池板和铅酸蓄电池两种方式,提高了设备野外工作的稳定性。硬件设计采用Cortex-m3内核的stm32f103作为MCU,相比于ARM系列,功耗降低了1/4,速度快了1/3。软件设计开发了数据采集、无线通信和在线访问等程序,通过浏览器,即使在远离监测点的异地,也能够实时查看设备状态和访问历史数据。经实践验证,系统能实现数据的稳定传输,适合农作物生长参数的实时监测。     

平面弹性欠驱动并联机构刚度特性分析

根据多维运动模拟的需求,提出一种包含螺旋圆弹簧(圆柱形螺旋弹簧)的欠驱动运动模拟台。利用螺旋圆弹簧的轴向和横向刚度约束运动模拟台的工作空间,通过两个驱动分支和动力耦合实现其3个自由度的运动。将螺旋圆弹簧等效为RePeRe弹性运动支链,进而将并联运动模拟台等效为2RPR/2RePeRe弹性欠驱动机构,并利用Grassmann线几何分析该机构的输入选取原则。基于旋量及其迁移公式,建立等效机构的运动学模型。借助于影响系数和虚功原理,对该弹性欠驱动机构进行静力学分析,得到其静力学约束方程。最后,建立了该机构的连续非线性柔度模型,并以此为基础得到其方向刚度模型。     

浅析我国植物检疫领域标准样品研究现状

本文介绍了标准样品研制工作在我国植物检疫领域的发展历程,梳理分析了已发布/立项标准样品的相关数据,结合作者单位在该领域研制标准样品实践经验,对植物检疫领域标准样品存在的问题进行了讨论,以供今后工作参考.     

利用重组自交系高密度遗传图谱定位水稻芽期耐冷QTL

为定位水稻芽期耐冷QTL,本实验以双季超级稻品种‘五丰优T025’的双亲‘五丰B’和‘昌恢T025’杂交衍生的重组自交系(recombinant inbred lines, RILs)群体为材料,对10℃低温处理的水稻幼芽的存活率、根数、根长和芽长进行了测定。利用QTL Icimapping v4.2软件,共检测到3个控制芽期耐冷性QTL：qRL1、qRL2和qBL6,分别位于第1、2、6染色体上,LOD值分别为2.98,2.51和5.26,分别解释表型变异的10.54%,8.67%和14.04%,其增效等位基因均来自于亲本‘昌恢T025’。这些QTL定位在6.75k~40.05 kb染色体区间,为后续利用这些QTL进行分子标记辅助,选育芽期耐冷籼稻新品种奠定了基础。此外,检测到13对影响水稻芽期耐冷上位性互作QTL,分布在所有12条染色体,其中第3染色体与第8染色体之间互作位点可解释的表型变异率达到21.77%,表明上位性互作QTL在调控水稻芽期耐冷过程中也发挥了重要作用。