The x-ray structure of chicken skeletal muscle troponin C (TnC), the Ca2+-binding subunit of the troponin complex, shows that the protein is about 70 angstroms long with an unusual dumbbell shape. The carboxyl and amino domains are separated by a single long alpha helix of about nine turns. Only the two high-affinity Ca2+-Mg2+ sites of the COOH-domain are occupied by metal ions resulting in conformational differences between the COOH- and NH2-domains. These differences are probably important in the triggering of muscle contraction by TnC. Also the structure of TnC is relevant in understanding the function of other calcium-regulated proteins, in particular that of calmodulin because of its strong similarity in amino acid sequence. 相似文献
1. Wheat, triticale 131 or triticale 72‐S could replace up to half the maize in broiler diets without affecting performance. Replacing more of the maize on either an equal nitrogen basis or a weight basis adversely affected performance.
2. Triticale 72‐S appeared to be superior to triticale 131 as a direct replacement for maize, but both grains were inferior to maize as energy sources. 相似文献
1. Dry rabbit excreta were found to contain 188.1 g crude protein, 6.6 g non‐protein nitrogen, 0.3 g ammonia, 89.8 g moisture, 8.0 g ether extractives, 135.2 g crude fibre, 266.9 g ash and 19.18 MJ gross energy/kg. Methionine and lysine contents were 3.95 and 4.29 g/100 g protein, respectively.
2. In one experiment, 0, 50, 100 or 200 g rabbit excreta/kg were substituted for maize in a broiler diet. Chickens receiving the diet containing 200 g rabbit excreta/kg gained significantly less weight to 8 weeks of age than those receiving other diets.
3. In a second experiment, 0, 100, 150 or 200 g rabbit excreta/kg were substituted primarily for maize in a broiler diet to provide equal energy and protein contents. Weight gains to 8 weeks of birds receiving these diets did not differ significantly. The uncorrected metabolisable energy content of the excreta was 9.15 MJ/kg.
4. There were no gross pathological changes in the birds fed on the experimental diets. 相似文献
1. The nitrogen‐corrected metabolisable energy contents of the triticale varieties Multi Graze, Graze Grain 70, 204 and 131 were 12.28, 12.69, 12.37 and 12.03 MJ/kg respectively.
2. Increasing proportions (0, 25, 50 or 75%) of the maize of a diet were replaced by triticale 131 to provide diets of equal energy and nitrogen contents for broilers. These diets resulted in weight gains of 1.775, 1.782, 1.713 and 1.808 kg respectively at 8 weeks and there were no significant differences among efficiencies of food conversions.
3. Dressed carcass weights as percentages of body weight were 74.38, 75.01, 73.98 and 74.50 respectively and did not differ significantly.
4. Triticale in the diet did not seem to influence the relative weights of either liver or gizzard.
5. The flavour of the meat seemed to become less acceptable as the triticale in the diet increased but there were no significant differences in tenderness and juiciness of the meat caused by the treatments. 相似文献
Our previous study evidenced that the 3D CORAGRAF loaded with PLGA microsphere constitutes PDGF-BB can support cell attachment and proliferation and can induce an osteogenic commitment of mesenchymal stromal cells in the in vitro condition. However, how this construct can perform in pathophysiological conditions in terms of repairing critical bone defects is yet to be understood. A study was therefore conducted to investigate the regeneration potential of calvaria critical-size defects using CORAGRAF + PLGA with PDGF-BB + mesenchymal stromal cells (MSCs) in a rat model. A 5 mm critical bone defect was created on calvaria of 40 male Sprague-Dawley rats. CORAGRAF incorporated either with or without PDGF-BB and seeded with rat bone-marrow-derived MSCs was implanted at the defect region. The bone regeneration potential of implanted constructs was assessed using micro-CT imaging and histological staining in weeks 4 and 8. The micro-CT images indicated a significant closure of defects in the cranial bone of the rats treated with 3D CORAGRAF + PLGA with PDGF-BB + MSCs on week 4 and 8 post-implantation. This finding, further supported with the histology outcome where the rat cranial defect treated with CORAGRAF + PLGA with PDGF-BB + MSCs indicated neo-bony ingrowth with organized and mature bone-like morphology as compared with other groups. The previous in vitro results substantiated with our pre-clinical findings demonstrate that the combination of CORAGRAF + PLGA with PDGF-BB + MSCs could be an ideal construct to support bone regeneration in critical bone defects. Hence, this construct can be further investigated for its safety and efficacy in large animal models, or it can be skipped to human trial prior for commercialization. 相似文献