聚类分析在我国果树研究中的应用及问题分析

对近１０年来聚类分析在我国果树研究中的应用情况进行了简要总结。涉及十余种果树及系统聚类和模糊聚类两种聚类分析方法。文中对有关问题进行了分析和讨论。     

温室池水环境与罗非鱼运动性气单胞菌败血症爆发的关系

为阐明温室池水环境对罗非鱼运动性气单胞菌败血症发生的影响,对5年监测所得的发病池的95个水样和无病池的78个水样的水环境因子值进行了统计分析。结果表明,虽然发病池和无病池的水温平均值和pH平均值差异均不显著(p＞0.05),但在18～27 ℃范围内,发病池出现的百分率随水温升高而增加;发病池的溶解氧、总氨氮、分子氨氮、亚硝酸盐氮、有机物化学耗氧量的平均值分别为2.85±1.45、2.48±0.99、0.067±0.048、0.361±0.233、39.56±20.31 mg/L;无病池的溶解氧、总氨氮、分子氨氮、亚硝酸盐氮、有机物化学耗氧量的平均值分别为3.41±1.39、0.94±0.76、0.025±0.025、0.105±0.066、20.62±9.73 mg/L。发病池的溶解氧平均值显著低于无病池(p＜0.05),发病池的总氨氮、分子氨氮、亚硝酸盐氮、有机物化学耗氧量的平均值分别极显著高于无病池(p＜0.01)。根据发病池和无病池出现的百分率随各环境因子变化的趋势可以得出结论：高水温、低溶解氧、高氨氮、高分子氨氮、高亚硝酸盐氮和高有机物化学耗氧量的池水更容易诱发凡隆气单胞菌温和生物型引起的罗非鱼运动性气单胞菌败血症。     

SIRT1 promotes autophagy of pancreatic cancer cells induced by hypoxia via regulating FOXO1/RAB7 signaling pathway

AIM: To investigate the effect of SIRT1 on the autophagy of pancreatic cancer cells under hypoxia condition, and to analyze the underlying mechanism of regulating FOXO1/RAB7 signaling pathway. METHODS: Western blot and immunofluorescence methods were used to determine the expression of SIRT1 in the pancreatic cancer cells. The small interfering RNA targeting SIRT1 and SIRT1 over-expression plasmid were transfected into the pancreatic cancer Panc-1 cells. Confocal microscopy was used to detect the LC3 expression. Western blot was used to analyze the protein levels of LC3, p62 and FOXO1/RAB7 signaling pathway-related molecules. Co-immunoprecipitation was used to detected the protein interaction between SIRT1 and FOXO1. RESULTS: The expression level of SIRT1 in the nucleus of Panc-1 cells was increased under hypoxia condition. Compared with negative control under hypoxia condition, knock-down of SIRT1 expression attenuated the autophagy flux in the pancreatic cancer Panc-1 cells (P<0.05). Over-expression of SIRT1 increased the protein levels of FOXO1 and RAB7. On the contrary, knock-down of SIRT1 expression inhibited the protein levels of FOXO1 and RAB7. The protein interaction between SIRT1 and FOXO1 in the pancreatic cancer cells was observed. CONCLUSION: SIRT1 in pancreatic cancer Panc-1 cells under hypoxia condition is over-expressed in the nucleus. Down-regulation of SIRT1 inhibits autophagy and its mechanism may be related to FOXO1/RAB7 signaling pathway.     

刑侦犬锁定"苦行僧"毒贩--云南腾冲"3·12"特大跨国贩毒案侦破纪实

这是一起在云南警方组织开展的“天网三号”禁毒专项行动中多警种协作、群众踊跃参与、警犬投入侦破的典型案例:2006年3月12日,当3名嫌疑人面对从两公里外查获的3背包沉甸甸的毒品海洛因及其他赃物拒不认罪、案件侦查陷入僵局时,刑侦犬“安普贵”奉命出击,把毒品和毒贩锁定在一     

百合小球培养复壮试验初报

90年代以来,随着花卉业的发展,我国开始进行观赏百合的大面积生产.但由于目前我国还不能进行百合种球的繁殖生产,每年都要从荷兰进口几百万个种球,不仅花费大量外汇,还存在其它方面的一些问题[1].建立我们自己的种球繁殖基地,不仅可以降低生产成本,还可以打破进口种球一统天下的局面.百合小球培养2年后成为能开花的商品种球[2],本试验旨在研究各种来源的百合小球培养后种球周径的增长情况,为以后进行百合种球的规模生产提供依据.     

棉田土壤养分精准管理初探

 应用地理信息系统 (GIS)结合土壤养分状况系统研究法探讨了一定农业生产条件下棉田土壤养分空间变异及其在推荐施肥中的应用。结果表明 ,棉田土壤养分的空间变异与前茬作物的种植利用方式有很大关系。网格取样技术和变量施肥技术是提高肥料有效性的有希望的手段 ,这些技术能使肥料以合适的用量施到需要的地方。平衡施肥示范结果显示根据土壤养分状况提出的推荐施肥 ,比当地常规施肥每公顷降低肥料成本 657.4元 ,籽棉产量从每公顷 4350kg增加到 52 1 2 .5kg,增产 1 9.8% ,增产所增加的收入每公顷 4657.5元 ,净增收益 531 4.9元。     

湖北省土壤各级颗粒的磁化率

本试验对湖北省六种土壤五个粒级(>1,1-0.5,0.5-0.25,0.25-0.15.0.15-0.1mm)的磁化率进行了测定。结果表明:红壤、棕红壤、潮土和黄棕壤表土层磁化率大于母质层磁化率,而紫色土和黄褐土表土层磁化率小于下部土层的磁化率。棕红壤表土层磁化率小于淀积层磁化率。红壤、棕红壤、黄棕壤和黄褐土母质层磁化率随颗粒变细而明显增加,但这一现象在潮土、紫色土的母质层确不明显。0.25-0.15mm粒级是黄棕壤、红壤、棕红壤、潮土、紫色土磁化率随颗料大小变化的一个转折点。     

套袋与不套袋对苹果食用安全性的影响

在果园常规管理模式下,比较了果实套袋与不套袋对苹果食用安全性的影响。通过对生产中允许使用以及国家在果树上禁限用的31种农药和重金属总砷的分析,结果表明,不套袋苹果中检出的农药残留量及总砷含量稍高于套袋,但从果实生长初期到采收期,无论套袋与否,果实内残留的农药和总砷含量均远低于国家标准规定的限量值。因此,在常规管理模式下,不套袋对苹果安全食用没有影响;而苹果套袋应对果袋进行规范管理,避免其对苹果的质量安全出现负面影响。     

基于膜上开沟方式的穴苗移栽机设计

针对膜上穴苗移栽机存在的效率低以及膜上覆土不实的问题,提出了一种膜上开沟栽植的新方法。同时,利用三维设计软件设计了移栽机的整机结构,介绍了移栽机的工作原理,并对关键部件结构与参数进行了设计,实现了膜上移栽机的高效移栽和膜上覆土,旨在为高效膜上移栽机的下一步研究提供参考。     

Induction of antiviral resistance and stimulary effect by oligochitosan in tobacco

Oligochitosan was applied by spraying it on tobacco leaves for inhibition of tobacco mosaic virus (TMV). The maximum inhibition of TMV by oligochitosan was observed when inoculation occurred at 24 h after spraying 50 μg ml⁻¹ oligochitosan. The production of H₂O₂ and NO in epidermal tobacco cells induced by oligochitosan was investigated by epidermal strip bioassay and LSCM, using cell permeable fluorophore diaminofluorescein diacetate (DAF-2D) and 2′,7′-dichlorofluorescin diacetate (H₂DCF-DA), respectively. Epidermal tobacco cells treated with oligochitosan resulted in a strong increase of intracellular NO and H₂O₂. Oligochitosan and NO donor sodium nitroprusside (SNP) induced the defense reaction against tobacco mosaic virus (TMV), and increased phenylalanine ammonia-lyase (PAL) activity. Co-treatment of the tobacco cells with oligochitosan and NO scavenger CPTIO blocked the inducing resistance. The results indicated that the defense response induced by oligochitosan was connected with NO pathway.