Genetic variation in seed production and its components in four cultivars of the pasture grass Setaria sphacelata

Variation in seed production and its components was investigated between and within four cultivars of the pasture grass Setaria sphacelata, in two experiments over two years, as a basis for future cultivar improvement. The study sought to determine the basis for the considerable differences in seed production of the four cultivars Nandi, Narok, Solander and Kazungula, to determine the extent of genetic variation in the four cultivars in attributes which contribute to seed yield, and to investigate genotypic consistency in seed production over years and seasons. Each experiment comprised 50 genotypes of each cultivar. In the first experiment, plants were harvested a set number of days after median flowering date whereas in the second experiment, which was unreplicated, each genotype was harvested a set number of days after it had flowered. In the first experiment, seed yields were generally highest for Kazungula, lowest for Narok and intermediate for Nandi and Solander. All measured attributes contributing to seed yield exhibited a high order of variation between and within cultivars, but the basis for the large difference in seed yield per plant between cultivars was tiller fertility rather that total tiller number. Averaged over the four harvests, there was a six fold to > 100–fold intra-cultivar genetic range in seed production, associated with differences in tiller fertility, which were associated with differences in date of first flowering. Broad sense heritability for seed yield averaged 0.68 for the four cultivars and showed little change over the four harvests. Genotypes which produced high seed yields in summer were also more productive of seed in autumn and the 0ore productive genotypes in the first year were also more productive in the second year. Cultivars differed in the relative importance of factors which contributed to the high seed yield of high-yielding genotypes. In the second experiment, genotypes with a high seed yield also generally had the highest tiller fertility, even though all genotypes were harvested the same number of days after first flowering. Within-cultivar correlations in seed yield between the two experiments were generally significant and the elite 20% of genotypes from this experiment had 1.2–2.9 times the seed yield of the same genotypes with a very different harvesting regime in the first experiment. It is concluded that opportunities exist in all four cultivars for improvement in seed production and that the selection criterion offering the best opportunity for advance would be fertile tiller number. In Narok, Solander and Nandi, this would result in increased tiller fertility, whereas in Kazungula, it would result in an increase in total tiller number. This revised version was published online in July 2006 with corrections to the Cover Date.     

Observations sur l'emploi des nématicides endothérapiques contre le genre Meloidogyne en horticulture florale

L'efficacité et la rentabilité des traitements nématicides endothérapiques dirigés contre les nématodes du genre Meloidogyne sont étudiées à l'aide de divers exemples. Les possibilityés de réduction des doses d'emploi sont envisageées sous trois angles, en fonction du ravageur, du produit et de la plante. Les traitements curatifs de rattrapage introduisent la notion de sensibilite des différents stades larvaires. Leur opportunité est également étudiée en fonction de la vitesse et de l'impottance du rétablissement des cultures. Les traitements périodiques et préventifs s'appuient sur la connaissance du mode et de la durée d'action des produits et sur divers facteurs liés à la physiologie de l'hôte végétal. Ceci conduit à«hiérarchiser» les deux types de traitement.     

Detection of Aphanomyces invadans and epizootic ulcerative syndrome in the Murray‐Darling drainage

Epizootic ulcerative syndrome was diagnosed, and the presence of Aphanomyces invadans confirmed, from an outbreak of clinical disease in wild‐caught bony bream (Nematalosa erebi) from the Darling River near Bourke, in New South Wales, Australia, during 2008. This confirms a significant extension of the agent beyond its historical range.     

Involvement of ATP‐sensitive K+ channels in the peripheral antinociceptive effect induced by ketamine

ObjectiveTo investigate the contribution of K⁺ channels on peripheral antinociception induced by ketamine.Study designProspective experimental study.Animals110 male Wistar rats weighing 160–200 g.MethodsThe paw pressure required to elicit limb flexion was designated as the nociceptive threshold. Hyperalgesia was induced by intraplantar injection of prostaglandin E₂. All drugs were administered locally into the right hind paw of rats. Ketamine was administered into the right hind paw 2 hours and 55 minutes after local injection of PGE₂. Tetraethylammonium was administered 30 minutes prior to ketamine and the other K⁺ channel blockers, glibenclamide, dequalinium and paxilline, were administered 5 minutes prior to ketamine.ResultsProstaglandin E₂ (2 μg per paw) induced hyperalgesia. Ketamine (10, 20, 40 and 80 μg per paw) elicited a local peripheral antinociceptive effect that was antagonized by a specific blocker of ATP‐sensitive K⁺ channels, glibenclamide (20, 40 and 80 μg per paw). In another experiment, the non‐selective voltage‐dependent K⁺ channel blocker tetraethylammonium (30 μg per paw) and small and large conductance blockers of Ca²⁺‐activated K⁺ channels, dequalinium (50 μg per paw) and paxilline (20 μg per paw), were ineffective at blocking the effect of a local ketamine injection.Conclusions and clinical relevanceAnalysis of these results provides evidence that ketamine, may in part, induce peripheral antinociceptive effects by ATP‐sensitive K⁺ channel pathway activation.     

Skeletal deformities associated with nutritional congenital rickets in newborn lambs

Abstract

CASE HISTORY: A group of 545 pregnant rising 2-year-old Coopdale ewes on a Southland sheep farm were grazed over winter on a fodder beet (Beta vulgaris) crop. Subsequently, 45 out of approximately 750 lambs were born with a variety of skeletal deformities, including shortened limbs, varus and valgus angular limb deformities, palmar grade stance and cranial bowing of the carpus. Analysis of the crop showed the fodder beet contained a low percentage of phosphorus. In addition, 60 out of 460 rising 2-year-old ewes that had been grazed on the fodder beet crop as 1-year-olds had incisor abnormalities and malocclusion.

PATHOLOGICAL FINDINGS: Two affected lambs (1-day-old and 3-days-old) with representative clinical signs examined postmortem were found to have markedly enlarged costochondral junctions, and noticeably enlarged long bone metaphyses. In addition, one lamb had a dense band of metaphyseal sclerosis beneath the physes of all long bones examined. Histopathological findings included small islands and columns of chondrocytes and eosinophilic cartilage matrix present in the metaphysis. Metaphyseal trabeculae were disorganised and often lined by accumulations of pale pink osteoid; similar pale pink osteoid was also present in the cortices. Unerupted molar teeth in the affected lambs lacked a layer of enamel, and the dentine was irregular with globular basophilia.

DIAGNOSIS: The gross and histopathological lesions were consistent with a diagnosis of rickets.

CLINICAL RELEVANCE: Nutritional congenital rickets has not been previously diagnosed in sheep, but is a recognised disease of human infants with vitamin D deficient mothers. The rickets in affected lambs was most likely associated with phosphorus deficiency as a result of the pregnant ewes grazing fodder beet during gestation. While vitamin D deficiency was not definitively ruled out in these cases, practitioners are alerted to the possible effects of feeding phosphorus-deficient fodder beet to ewes for long periods during gestation and to 1-year-old sheep during important growth periods.     

Climate and vegetation history of the midcontinent from 75 to 25 ka: A speleothem record from crevice cave, missouri, USA

Four Missouri stalagmites yield consistent overlapping records of oxygen and carbon isotopic changes and provide a climate and vegetation history with submillennial resolution from 75 to 25 thousand years ago (ka). The thorium-230-dated records reveal that between 75 and 55 ka, the midcontinental climate oscillated on millennial time scales between cold and warm, and vegetation alternated among forest, savanna, and prairie. Temperatures were highest and prairie vegetation peaked between 59 and 55 ka. Climate cooled and forest replaced grassland at 55 ka, when global ice sheets began to build during the early part of Marine Oxygen Isotope Stage 3.     

Femtosecond dynamics of electron localization at interfaces

The dynamics of two-dimensional small-polaron formation at ultrathin alkane layers on a silver(111) surface have been studied with femtosecond time- and angle-resolved two-photon photoemission spectroscopy. Optical excitation creates interfacial electrons in quasi-free states for motion parallel to the interface. These initially delocalized electrons self-trap as small polarons in a localized state within a few hundred femtoseconds. The localized electrons then decay back to the metal within picoseconds by tunneling through the adlayer potential barrier. The energy dependence of the self-trapping rate has been measured and modeled with a theory analogous to electron transfer theory. This analysis determines the inter- and intramolecular vibrational modes of the overlayer responsible for self-trapping as well as the relaxation energy of the overlayer molecular lattice. These results for a model interface contribute to the fundamental picture of electron behavior in weakly bonded solids and can lead to better understanding of carrier dynamics in many different systems, including organic light-emitting diodes.