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排序方式: 共有925条查询结果,搜索用时 15 毫秒
1.
2.
Influence of death struggle on the structural changes in chub mackerel muscle during chilled storage
Masashi Ando Masao Joka Satoshi Mochizuki Koh-Ichi Satoh Yasuyuki Tsukamasa Yasuo Makinodan 《Fisheries Science》2001,67(4):744-751
ABSTRACT: Chub mackerel (34–35 cm, approximately 500 g), which were caught by fishing with a rod and line at the Bungo Channel, Oita prefecture, were rested overnight in a fish preserve and either killed by decapitation (control group) or allowed to struggle in air for 30 min (struggled group). Muscle samples were excised every 4 h, and measurements on breaking strength and histological observations were done for both groups. The breaking strength of muscle in the control group was significantly higher than that in the struggled group, whereby a decrease in breaking strength was delayed for 12 h compared to the struggled group. Light microscopy showed space extension among muscle cells in association with a decrease in breaking strength. Especially in the struggled group, the extended area was larger and the difference in area was significant at the time when breaking strength showed a significant difference. Using electron microscopy, the extended area showed cut and/or disappeared collagen fibrils. From these results, it was demonstrated that struggling to death promoted the degradation of collagen fibrils and the weakening of connective tissue and, resultantly, led to the faster softening of muscle of chub mackerel. 相似文献
3.
T Sawada H Tamada T Inaba J Mori 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):1061-1062
The possibility of estrus prevention in the queen by the oral administration of chlormadinone acetate was examined. The animals used were 29 mature and 15 immature queens. For 16 mature females, 4-12.5 mg was given daily by mouth for 7 days every 3 months. Ten of the 16 queens given this treatment came into estrus within 4 months of the first treatment. For 28 females including the immature, 2-12.5 mg was given once a week throughout the experiment. This treatment prevented estrous activity for at least 1 year. In the queens in this study, the side effects were not observed excepting an increase in body weight during treatment. Our results showed that oral administration of this drug weekly is safe and reliable for long-range prevention of estrus in queens. 相似文献
4.
Hideki OGISO Masashi FUJINAGA Hideki SAITO Toshiaki TAKEHARA Shigeru YAMANAKA 《Journal of General Plant Pathology》2002,68(4):292-299
One hundred and sixteen isolates of Fusarium oxysporum f. sp. lactucae obtained from 85 fields in three crisphead lettuce-producing areas in Nagano Prefecture, Japan were typed for races using
differential cultivars Patriot, Banchu Red Fire and Costa Rica No. 4. They were also grouped into vegetative compatibility
groups (VCGs) using complementation tests with nitrate non-utilizing (nit) mutants. Two California strains reported as F. oxysporum f. sp. lactucum, a type culture of F. oxysporum f. sp. lactucae, and 28 avirulent isolates of F. oxysporum obtained from crisphead lettuce were included for comparison. Among Nagano isolates, 66 isolates were identified as race 1,
and 50 as race 2. Race 1 strains derived from Shiojiri and Komoro cities and race 2 from Kawakami village and Komoro city.
All isolates of race 2 were biotin auxotrophs, and the race could be distinguished based on its requirement for biotin on
minimal nitrate agar medium (MM). Pathogenic isolates were classified into two VCGs and three heterokaryon self-incompatible
isolates. Strong correlations were found between race and VCG. All the race 1 strains were assigned to VCG 1 except self-incompatible
isolates, and all the race 2 strains to VCG 2. The 28 avirulent isolates of F. oxysporum were incompatible with VCG 1 and VCG 2. California strains was vegetatively compatible with VCG 1, and they were assigned
to race 1. Based on vegetative compatibility, these two races of F. oxysporum f. sp. lactucae may be genetically distinct, and F. oxysporum f. sp. lactucae race 1 is identical to F. oxysporum f. sp. lactucum.
Received 7 May 2002/ Accepted in revised form 6 September 2002 相似文献
5.
Minimal Set of Metabolic Pathways Suggested from the Genome of Onion Yellows Phytoplasma 总被引:1,自引:0,他引:1
6.
Hitomi NAKABAYASHI Yasuyuki YAMAJI Satoshi KAGIWADA Masashi UGAKI Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(2):173-176
The complete nucleotide sequence was determined for genomic RNA of White clover mosaic virus (WClMV-RC) isolated from red clover (Trifolium pratense) in Japan, It is 5843 nucleotides in length, excluding the poly(A) tail at the 3' terminus. Similar to other potexviruses,
it contains five open reading frames (ORFs 1 through 5), which putatively encode an RNA-dependent RNA polymerase (RdRp) (147
kDa), a triple gene block (TGB) (26 kDa/13 kDa/7 kDa), and a coat protein (CP) (22 kDa), respectively. The deduced amino acid
sequence of the WClMV-RC CP was identical to that of WClMV-O, one of two New Zealand isolates, but only 85% identical to that
of WClMV-M, the other New Zealand isolate, because of heterogeneity in the C-termini of CP amino acid sequences. The implication
of this CP heterogeneity is discussed.
Received 30 August 2001/ Accepted in revised form 11 January 2002 相似文献
7.
Histological and immunohistochemical evaluation of granulosa cells during different stages of folliculogenesis in bovine ovaries
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APP Teh UZ Izzati K Mori N Fuke T Hirai G Kitahara R Yamaguchi 《Reproduction in domestic animals》2018,53(3):569-581
Bovine granulosa cells (GC) vary in their morphological aspect during different stages of folliculogenesis. In this study, 10 morphologically normal bovine ovaries were collected to study the structural aspects of different stages of GC using intermediate filament protein antibodies including cytokeratin AE1/AE3 (AE1/AE3), vimentin, nectin‐4 and desmin. Hormonal immunolocalization was assessed using the immunomarkers anti‐Müllerian hormone (AMH) and inhibin alpha. In addition, tumour markers and proliferation markers using c‐erbB‐2 oncoprotein and proliferating cell nuclear antigen, respectively, were investigated. The immunolabelling of AE1/AE3 in GC was strongest in the early follicle stage and gradually decreased when reaching the Graafian follicle stage. Its immunolabelling increased again as the stage progressed from stage I to stage III. The immunolabelling of inhibin alpha was inversely proportional to that of AE1/AE3 in the developing ovarian follicles as their immunolabelling is opposite to each other during folliculogenesis. AMH was immunopositive in almost all GC stages in different intensities and percentages, except for some negative staining in the atretic IV follicles. The atretic IV follicle is a unique type of atretic follicle that shows Call‐Exner body formation, which was mainly found in older cows in this study. The distinct patterns of immunoreactivity for various types of immunomarkers in the different GC stages will play an important role in diagnostic assistance of various follicle conditions, including cystic ovaries and GC tumours. 相似文献
8.
9.
Expression dynamics of bovine MX genes in the endometrium and placenta during
early to mid pregnancy
Takahiro SHIROZU Keisuke SASAKI Manabu KAWAHARA Yojiro YANAGAWA Masashi NAGANO Nobuhiko YAMAUCHI Masashi TAKAHASHI 《The Journal of reproduction and development》2016,62(1):29-35
MX belongs to a family of type I interferon (IFN)-stimulated genes, and the MX protein has
antiviral activity. MX has at least two isoforms, known as MX1 and
MX2, in mammals. Moreover, bovine MX1 has been found to have alternative
splice variants—namely, MX1-a and MX1B. In ruminants, IFN-τ—a type I IFN—is
temporarily produced from the conceptus before implantation and induces MX expression in the
endometrium. However, the expression dynamics of MX after implantation are not clear. In the
present study, we investigated the expression of MX1-a, MX1B and
MX2 in the endometrium and placenta before and after implantation along with the expression
of IFN-α, type I receptors (IFNAR1 and IFNAR2) and
interferon regulatory factors (IRF3 and IRF9). Pregnant uterine samples were
divided into five groups according to pregnancy days 14–18, 25–40, 50–70, 80–100, and 130–150. Tissue samples
were collected from the intercaruncular endometrium (IC), caruncular endometrium (C) and fetal placenta (P).
Although all the MX expressions were significantly higher in the IC and C at days 14–18,
presumably caused by embryo-secreted IFN-τ stimulation, their expressions were also detectable in the IC, C
and P after implantation. Furthermore, IFN-α expression was significantly higher in the IC.
RT-PCR indicated IFNAR1, IFNAR2, IRF3 and
IRF9 mRNA in all the tissues during pregnancy. These results suggest that all the
MX genes are affected by the type I IFN pathway during pregnancy and are involved in an
immune response to protect the mother and fetus. 相似文献
10.
The aims of this study were to develop a new real-time quantitative PCR (QPCR) assay based on IS900 for detection and quantification of Mycobacterium avium subsp. paratuberculosis (MAP) DNA in faeces, and to use this to detect infected sheep. Both the C and S strains of MAP were detected by the QPCR assay, and no cross reactions were detected with 51 other species of mycobacteria including 10 which contained IS900-like sequences. One copy of IS900 fragment cloned into plasmid pCR2.1 and 1 fg of MAP genomic DNA were consistently detected, while in spiked faecal samples the detection limit was 10 viable MAP per gram of ovine faeces. A total of 506 individual ovine faecal samples and 27 pooled ovine faecal samples with known culture results were tested. The QPCR assay detected 68 of 69 BACTEC culture positive individual faeces and there was a strong relation between time to detection in culture and DNA quantity measured by QPCR (r= -0.70). In pooled faecal samples, QPCR also agreed with culture (kappa=0.59). MAP DNA was detected from some culture negative faecal samples from sheep exposed to MAP, suggesting that the QPCR has very high analytical sensitivity for MAP in faecal samples and detects non-viable MAP in ovine faeces. None of the faecal samples from 176 sheep that were not exposed to MAP were positive in QPCR. This is the first report of a direct faecal QPCR assay that has similar sensitivity to a gold standard radiometric culture assay. 相似文献