反刍动物高能量饲料添加剂脂肪酸钙制作工艺参数筛选

以猪脂肪、氢氧化钠、氯化钙为原料,在实验室条件下探索脂肪酸钙的加工工艺和加工过程。利用正交试验设计(L_(16)4~5)对脂肪酸钙的加工工艺系数进行反复筛选,探索脂肪酸钙的最佳加工工艺参数。结果表明,脂肪酸钙的最佳加工工艺参数为氢氧化钠用量占脂肪重的27%、氯化钙用量占脂肪重的25%、皂化反应时间为5h、复分解温度为90℃、用水量为脂肪的4倍。     

肇庆市红火蚁防控措施研究

广东于2004年发现了红火蚁,对生态和生产生活造成了严重影响,全省各地开始加强对红火蚁的防控.简述了肇庆市红火蚁防控概况及防控措施,总结了防控成效及存在的主要问题,提出了防控策略,以期为肇庆市红火蚁的防治提供科技支撑与保障.     

芸薹属及其他异源多倍体植物的基因表达特征研究进展

远缘杂交及异源多倍化导致许多重要作物的起源与进化，而芸薹属栽培异源四倍种是研究作物异源多倍化的模式系统之一。异源多倍体是如何调控及协调来自不同二倍体祖先的不同基因组的遗传行为及基因表达，是过去二十年间的研究热点和重要的生物学问题。利用不断发展的分子生物学技术，一方面揭示出芸薹属及其他多倍体物种基因组表现出动态性质，即在形成初期及长期进化过程中持续发生遗传及表观遗传的变化；另一方面发现异源多倍化过程中伴随着大量的基因表达模式改变，包括非加性表达、超亲表达、表达水平显性、部分同源偏向表达、基因剂量平衡效应等现象。上述基因组结构、表观遗传改变以及基因表达模式的调控，使新产生的多倍体得以成功进化为新物种。     

Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver

Goose fatty liver is a specific type of nonalcoholic fatty liver that is protected from harmful effects associated with severe steatosis. Our previous findings suggest that suppression of the complement C5 may be relevant, but the mechanism is unclear. Therefore, in this study, we first verified the expression pattern of complement genes (including C5) during goose fatty liver formation and then determined the liver fat content and fatty acid composition by high-performance liquid chromatography (HPLC), followed by selecting the differential metabolites to treat HepG2, goose and mouse primary hepatocytes, aiming to explore the mechanism of C5 and inflammation suppression in goose fatty liver. The data confirmed the suppression of complement genes (including C5) in goose fatty livers. Moreover, fat content was significantly higher in fatty liver versus normal ones, with oleic acid and palmitic acid dominantly accounting for the difference. In line with this, high concentration of palmitate led to down regulation of C5 expression in goose primary hepatocytes whereas upregulation in mouse primary hepatocytes and HepG2 cells. In conclusion, regulation on C5 expression by fatty liver related factors including high level of palmitic acid may contribute to the protection of goose liver from severe hepatic steatosis.     

猫鼻气管炎病毒、怀状病毒、泛白细胞减少症病毒的分离及其其形态学观察

根据病毒的理化特性，从猫三联活疫苗中分离出猫鼻气管炎、猫西洋太和猫泛白细胞减少症病毒；筛选出每种病毒敏感的细胞，并将该3种病毒分别分别在其中传代与扩增；通过电镜观察此3种病毒的形态学特征及其在宿主细胞内增殖部位、分布情况等，并以此对猫3种病毒进行了初步鉴定。     

Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.     

水稻条纹病毒在玉米和水稻上流行差异分析

为了明确水稻条纹病毒(RSV)在水稻和玉米上发生程度差异明显的原因,从一个侧面了解RSV流行本质,2004年在洪泽进行田间试验。采用黄盘诱集、盘扑、盘刮、肉眼计数等方法比较武育粳3号和掖单13上灰飞虱侵入和消长动态,结果表明两者均只有一个成虫侵入高峰,单位面积迁入虫量前者是后者的3.6倍,单株平均虫量相近。Dot-ELISA法测定灰飞虱带毒率为40%,成虫迁移扩散高峰期22d内两者接毒量约为每天百株137头带毒虫。武育粳3号有二代若虫发生,掖单13则无。逐日调查发病进程,结果显示水稻发病率为60%,玉米为0。室内抗性鉴定,掖单13对RSV的抗性比武育粳3号高4个级别。综合以上结果,寄主品种抗性决定了RSV在水稻和玉米上流行状况,二代若虫重复侵染是RSV在水稻上重发的另一主要原因。     

宁化县思茅松毛虫发生情况分析

调查了思茅松毛虫的生物学特性,并对大发生原因进行了分析和探讨,同时提出了对该虫的防治措施。     

奶牛布鲁氏菌病的病原分离鉴定及血清学研究

通过细菌分离、动物试验、细菌学与血清抗体之间相互关系、温度与感染动物之间相互关系等分离一例奶牛布鲁氏菌。发现细菌学与血清抗体呈平行关系，试管凝集反应与补体结合反应存在一定差异，感染动物14天出现波浪热，持续到剖杀前(31天)。     

钢筋混凝土结构锈蚀损伤定位

对钢筋混凝土结构锈蚀损伤定位进行了研究,以频率变化平方比作为锈蚀损伤参数,从理论上证明该参数是锈蚀损伤位置的函数。锈蚀钢筋混凝土结构试验结果表明预测的损伤位置和实际的损伤位置较一致,模态参数用于检测混凝土结构构件中的损伤,为大型结构的鉴定和评估提供了新的途径。