Replacement of fish oil in plant‐based diets for Pacific white shrimp,Litopenaeus vannamei,by stearine fish oil and palm oil

Stearine fish oil (SFO) and palm oil (PO) have emerged as promising alternatives for the replacement of fish oil (FO) in aquafeeds. This study evaluated the replacement of FO with alternative oils in practical diets for Litopenaeus vannamei. In a clear brackish water study (14.1 g/L) utilizing shrimp (0.29 ± 0.02 g, initial weight), FO was replaced by SFO at inclusion ratios of 100:0, 75:25, 50:50, 25:75, and 0:100 (FO:SFO) and PO as 90% of FO. After 55 days, no significant differences (p < 0.05) in final weight, growth, or survival of shrimp were observed. A second trial (8 weeks) in low‐salinity water (2.1 g/L) with shrimp (0.92 ± 0.02 g, initial weight) evaluated diets with 100% FO, 100% SFO, 90% PO, 90% soybean oil (SO), or 90% flaxseed oil (FXO) as a replacement for FO and four commercially produced diets with 2% of FO, SO, PO, or FXO. One treatment received half rations of the commercial FO diet, and one treatment was based entirely on natural productivity. Results show that the fatty acid profiles of the tail muscle conformed to the lipids of the feed, and highly unsaturated fatty acids (HUFAs) were preserved. Following 8 weeks of culture, there were no significant differences in production performance.     

Efficacy of abamectin against natural infections of gastrointestinal nematodes and lungworm of cattle with special emphasis on inhibited, early fourth stage larvae of Ostertagia ostertagi.

The anthelmintic efficacy of abamectin (avermectin B1) was evaluated against gastrointestinal nematodes, including Ostertagia ostertagi inhibited larvae and lungworm, in yearling crossbred beef heifers during late spring. The calves were grazed on contaminated pasture for 10 weeks and then held under conditions free of nematode infection for 3 weeks prior to allotment and treatment on 5 June. Thirteen calves were randomly assigned to two groups of six by restricted randomization on body weights; the extra lightest calf was assigned to the non-treated control group. Group 1 calves were treated with abamectin at 200 micrograms kg-1 body weight by s.c. injection and Group 2 calves were not treated; all were killed at 14 days after treatment. Ostertagia ostertagi was present in all controls; arithmetic mean numbers of adults, developing fourth stage larvae (L4) and inhibited EL4 were 7683, 605 and 36,102, respectively. Other nematode genera present in controls in sufficient numbers for the experiment were Haemonchus placei adults, Trichostrongylus axei adults, Cooperia spp. adults, Oesophagostomum radiatum adults, Bunostomum phlebotomum adults, Dictyocaulus viviparus adults and E5 (immature adults). Abamectin was highly effective (consistently greater than 99% efficacy and P less than 0.05) in removing all nematodes present in treated calves as represented in non-treated controls, including the primary target of Ostertagia ostertagi inhibited EL4. The lowest efficacy was 93.8%, against D. viviparus E5.     

Survival of Leptosphaeria maculans in soil on residues of Brassica napus in South Australia

The survival of Leptosphaeria maculans , which causes phoma stem canker (blackleg), on oilseed rape residues ( Brassica napus ) in South Australia was investigated. Using a quantitative polymerase chain reaction (PCR) assay for L. maculans DNA, the pathogen was mainly detected in the upper 5 cm of the soil profile, including residues on the soil surface. As the size of organic matter particles in the soil decreased, so did the quantity of L. maculans detected in them. To obtain representative data for a field, at least 30 subsamples needed to be collected over the 0·81 ha area studied. In a survey of 49 commercial fields in South Australia, most L. maculans was detected in fields 1 year after oilseed rape had been grown, with less detected after 2 years and negligible amounts 3 years or more after cropping. The diagnostic DNA-based assay for L. maculans reduced the time and cost of studying L. maculans survival in soil and increased the sensitivity and accuracy of results compared with estimates of propagule number of colony-forming units on a semiselective medium.     

Fractionated oral dosing and its effect on cyclophosphamide pharmacokinetics in dogs with high-grade multicentric lymphoma

Cyclophosphamide (CP) is an alkylating agent commonly included in multi-drug treatment protocols for canine cancer. As a prodrug, CP requires hepatic metabolism for activation to the intermediate compound 4-hydroxycyclophosphamide (4-OHCP) which then spontaneously forms alkylating phosphoramide mustard. CP is frequently administered in a fractionated manner, with the total dose given over multiple days. CP is reported to cause auto-induction of metabolism in humans, with faster CP clearance and relatively increased 4-OHCP formation following fractionated versus bolus dosing, however canine pharmacokinetic studies of CP dose fractionation are lacking. The study objective was to evaluate the pharmacokinetics of fractionated oral CP dosing at a dose of 200–250 mg/m² over 3 to 4 days in a prospectively identified population of cancer-bearing dogs. Plasma concentrations of CP and 4-OHCP were measured by ultra-high performance liquid chromatography tandem-mass spectrometry in eight dogs following the first and last doses to assess for auto-induction of CP metabolism. No significant difference in the rate of CP elimination between first and last doses were detected (0.73 ± 0.46 vs. 1.22 ± 0.5 h⁻¹; p = .125). Additionally, no significant difference in dose-normalized 4-OHCP exposure was identified between first and last doses (5.9 ± 2.1 vs. 7.9 ± 6.4 h × ng/ml; p = .936). These results suggest that fractionated dosing may not increase exposure to the active metabolite of CP in dogs as it does in humans. As such, standard bolus dosing and fractionated dosing may be equivalent in terms of bio-activation of CP in dogs administered a dose of 200–250 mg/m².     

Cotton-root distribution as a function of trickle irrigation emitter depth

Summary The objective of this study was to evaluate the distribution of cotton (Gossypium hirsutum cv GSA 71) root system under four depths of trickle irrigation emitter. Cotton was grown on an Amarillo fine sandy loam (fine loamy, mixed thermic Aridic Paleustalf) at Lubbock, Texas. Trickle irrigation was applied at the surface (0), 0.15, 0.3, or 0.45 m depths in amounts calculated using daily pan evaporation, crop coefficient, and crop development stage. Effects of depth of trickle irrigation were evaluated by measuring the cotton root-length density in 0.15 m increments to the 0.90 m depth at distances of 0, 0.25, and 0.5 m perpendicular to the cotton rows. The root-length distributions were not significantly (0.05%) different in the top 0.15 m when the surface irrigated treatment was compared with the 0.45 m irrigation depth treatment. Root-length densities for the 0.15 and 0.3 m treatments were not significantly different from one another nor were they different from treatments within the evaluated zones (0 to 0.3 m or 0.15 to 0.45 m). Root-length density within the 0.3 to 0.6 m zone, was not significantly different for the 0.45 m treatment compared with the surface irrigation treatment. Root-length density in the 0.6 to 0.9 m zone was not significantly different for any treatment. Emitter depth of trickle irrigation did not significantly influence the depth or distribution of cotton-root development.     

Diagnostic and public health investigation of Mycobacterium tuberculosis infection in a dog in Ontario,Canada

A 4-y-old, female mixed-breed dog was presented to the Ontario Veterinary College for further evaluation of multiple pulmonary and hepatic masses, intrathoracic lymphadenitis, and recent development of a pyogranulomatous pleural effusion. Along with other comprehensive tests, a thoracic lymph node biopsy was performed, and Mycobacterium tuberculosis complex infection was confirmed by real-time PCR. The dog’s condition declined post-operatively, and euthanasia was elected. Postmortem examination confirmed severe granulomatous pneumonia, hepatitis, intrathoracic and intraabdominal lymphadenitis, omentitis, and nephritis. Line-probe assays performed on samples collected postmortem confirmed the species as M. tuberculosis. 24-loci MIRU-VNTR genotyping, spoligotyping, and whole-genome sequencing revealed relations to known human isolates, but no epidemiologic link to these cases was investigated. Given the concern for potential human exposure during this animal’s disease course, a public health investigation was initiated; 45 individuals were tested for M. tuberculosis exposure, and no subsequent human infections related to this animal were identified. Our case highlights the need for more readily available, minimally invasive testing for the diagnosis of canine mycobacteriosis, and highlights the ability of canid species to act as potential contributors to the epidemiology of M. tuberculosis infections.     

地膜残留量对新疆棉田蒸散及棵间蒸发的影响

为探讨残膜对干旱区农田蒸散耗水特征的影响,在新疆阿克苏典型覆膜滴灌棉田开展2 a小区试验研究,设计0、225、450 kg/hm2共3种不同的地膜残留量,采用水量平衡法,微型棵间蒸发仪法,于主要生育时期测定并计算土壤含水量、蒸散量、棵间蒸发量、作物蒸腾量、棵间蒸发占蒸散的比例。结果表明:随着地膜残留量增加棵间蒸发量、棵间蒸发占蒸散的比例呈增大趋势,而蒸散量及作物蒸腾量则逐渐减小。与无残膜处理相比,225和450 kg/hm2处理全生育期田间无效耗水的棵间蒸发量分别增加了9.27和22.20 mm,棵间蒸发占蒸散的比例增幅分别为2.6%和6.1%,作物蒸腾量降低34.89和55.94 mm。在棉花生育期内,棵间蒸发占蒸散的比例(E/ET)与叶面积指数(leaf area index,LAI)呈幂函数关系,各处理间棵间蒸发占蒸散的比例对叶面积指数的响应差异不同,450 kg/hm2处理蒸发占蒸散的比例随着LAI的增加,曲线下降趋势较明显;无残膜处理棵间蒸发占蒸散的比例与LAI的决定系数最高,均在0.9以上。土壤水分利用率也随残膜量的增加依次降低,当残膜量由0增加到450 kg/hm2时,土壤水分利用率从28.25%降至24.91%,可见,残膜增大了农田的无效耗水,不利于土壤水分的有效利用。研究可为制定缓解或克服残膜危害的应变调控技术提供依据。