马铃薯致病疫霉拮抗细菌研究进展

为了解决马铃薯致病疫霉侵染马铃薯导致其死亡,进而造成马铃薯单位种植产量低的问题。本文从拮抗菌入手,归纳分析细菌类拮抗菌中的主要拮抗菌中芽孢杆菌、假单胞菌和粘细菌对马铃薯致病疫霉的抑制作用。同时,在总结前人研究的基础上,笔者认为在拮抗菌应用方面,可以采用多对一的新型平板对峙筛菌模式来模拟复杂条件下拮抗菌的拮抗作用,以期得到稳定的拮抗菌;并指出应从定植能力和根际微生物影响方面对拮抗菌实际应用的可行性进行研究;此外,提出未来对拮抗菌抑制马铃薯致病疫霉的探究应从表型层面的研究深入到分子层面,以期能成功从源头找寻抑制马铃薯致病疫霉的答案。     

Impact of grassland contract policy on soil organic carbon losses from alpine grassland on the Qinghai–Tibetan Plateau

Carbon storage in the soils on the Qinghai–Tibetan Plateau plays a very important role in the global carbon budget. In the 1990s, a policy of contracting collective grasslands to smaller units was implemented, resulting in a change from the traditional collective grassland management to two new management patterns: a multi‐household management pattern (MMP: grassland shared by several households without enclosures) and a single‐household management pattern (SMP: grassland enclosed and used by only one household). In 2016, 50 MMP and 54 SMP winter pastures on the Qinghai–Tibetan Plateau were sampled to assess the differences in soil organic carbon (SOC) between the two management patterns. Results showed that average SOC was significantly greater under MMP than under SMP, with an estimated 0.41 Mg C/ha/yr lost due to SMP following the new grassland contract. Based on the government's grassland policy, four grassland utilization scenarios were developed for both summer and winter pastures. We found that if the grassland were managed under SMP, likely C losses ranged between 0.31 × 10⁷ and 6.15 × 10⁷ Mg C/yr across the Qinghai–Tibetan Plateau relative to MMP, which more closely resembles pre‐1990s grassland management. Previous estimates of C losses have only considered land use change (with cover change) and ignored the impacts driven by land management pattern changes (without cover change). The new data suggest that C losses from the Qinghai–Tibetan Plateau are greater than previously estimated, and therefore that the grassland contract policy should be reviewed and SMP households should be encouraged to reunite into the MMP. These findings have potential implications for land management strategies not only on the Qinghai–Tibetan Plateau but also other grazing regions globally where such practices may exist.     

Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver

Goose fatty liver is a specific type of nonalcoholic fatty liver that is protected from harmful effects associated with severe steatosis. Our previous findings suggest that suppression of the complement C5 may be relevant, but the mechanism is unclear. Therefore, in this study, we first verified the expression pattern of complement genes (including C5) during goose fatty liver formation and then determined the liver fat content and fatty acid composition by high-performance liquid chromatography (HPLC), followed by selecting the differential metabolites to treat HepG2, goose and mouse primary hepatocytes, aiming to explore the mechanism of C5 and inflammation suppression in goose fatty liver. The data confirmed the suppression of complement genes (including C5) in goose fatty livers. Moreover, fat content was significantly higher in fatty liver versus normal ones, with oleic acid and palmitic acid dominantly accounting for the difference. In line with this, high concentration of palmitate led to down regulation of C5 expression in goose primary hepatocytes whereas upregulation in mouse primary hepatocytes and HepG2 cells. In conclusion, regulation on C5 expression by fatty liver related factors including high level of palmitic acid may contribute to the protection of goose liver from severe hepatic steatosis.     

播量对水稻生长发育及干物质积累的影响

用盆栽方法进行播量对寒地水稻生长发育及干物质积累影响试验,结果表明:稀播的秧苗发根力强、分蘖多、茎基宽、充实度高。播量200g/m2的产量最高。     

以花生为主体作物的立体种植高效农业配置

以覆膜花生为优势布局的小麦     

植物活体水分探测仪的研制及测试初报

利用NIM插件探测器及金硅面垒探头组装了植物活体水分探测仪;筛选并分析了仪器的最佳工作条件;检测了春小麦、玉米、苜蓿、豌豆、红豆草叶片鲜重的变化。结果表明;叶片水分随时间损失大的,其抗旱性差。叶片保水的秩序为:红豆草、豌豆>苜蓿>春小麦>玉米。     

绵羊实验性乳酸酸中毒研究——Ⅲ.肾功能和尿液的改变

将12只2～3岁健康绵羊分为Ⅰ组(3只)、Ⅱ组(6只)和Ⅲ组(3只),分别按2.5,5.0,10.0 g/kg体重的剂量,向瘤胃内注入50%D—L消旋体乳酸溶液,各处理组均出现酸中毒症状.乳酸酸中毒时,肾脏的损伤程度与瘤胃内注入乳酸的剂量水平有关.各处理组瘤胃内注入乳酸后,尿素氮显著升高.第Ⅰ组尿液pH下降迅速,恢复亦快;第Ⅱ组尿液pH下降和回升均较缓慢,而且出现大量蛋白质和血液;第Ⅲ组尿液除见第Ⅰ、Ⅱ组的变化外,还出现肌红蛋白、酮体和尿胆素原.     

农户舍饲奶牛血清钙及游离羟脯氨酸水平测定

采用氯胺T比色法和EDTANa_2滴定法,对重庆市农户舍饲奶牛不同泌乳阶段血清中游离羟脯氨酸与血清钙的水平进行 测定,旨在调查试验条件下奶牛血钙代谢情况以及骨营养不良发生的可能性。结果显示: 在农户全舍饲条件下,血清钙含量Ⅱ组显著低于Ⅴ组、Ⅵ组,其它各组间差异不显著;Ⅰ～ Ⅵ组血清羟脯氨酸含量分别为2.32±0.37μg/ml、2.36±0.59μg/ml、2.02±0.54μg/ml、1.90±0.44μg/ml、1.98±0.45μg/ml、1.79±0.18μg/ml,Ⅰ、Ⅱ组与Ⅵ组差异极显著。采样后2-3个月内进行临床观察,血清游离羟脯氨酸水平与骨营养不良发生率呈现一致性变化。而钙磷摄入不足,比例不当是主要原因。     

鱼粉中霉菌总数及霉菌毒素含量变化的研究

模拟大宗饲料原料鱼粉的贮存条件，比较不同贮存条件下鱼粉中霉菌总数、主要产毒菌的种类以及霉菌毒素黄曲霉毒素、赭曲霉毒素和呕吐毒素含量变化的情况。将温度设为：20℃±2，湿度设为：65％±5、75％±5、85％±5分别进行贮存．、检测时间为：第0、5、10、17、24、31和38d，利用国标通用检测方法进行霉菌总数和霉菌毒素含量变化的检测。结果表明．主要产毒菌种类有串珠镰刀菌、黄曲霉、构巢曲霉、纯绿青霉；其中串珠镰刀菌是优势菌，它的旺盛生长导致鱼粉中霉菌总数和呕吐毒素迅速超标．