Screening and Verification of Low Nitrogen Tolerant and Nitrogen Sensitive Cotton Germplasm

[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L^－1) and high (5 mmol·L^－1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient.     

Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver

Goose fatty liver is a specific type of nonalcoholic fatty liver that is protected from harmful effects associated with severe steatosis. Our previous findings suggest that suppression of the complement C5 may be relevant, but the mechanism is unclear. Therefore, in this study, we first verified the expression pattern of complement genes (including C5) during goose fatty liver formation and then determined the liver fat content and fatty acid composition by high-performance liquid chromatography (HPLC), followed by selecting the differential metabolites to treat HepG2, goose and mouse primary hepatocytes, aiming to explore the mechanism of C5 and inflammation suppression in goose fatty liver. The data confirmed the suppression of complement genes (including C5) in goose fatty livers. Moreover, fat content was significantly higher in fatty liver versus normal ones, with oleic acid and palmitic acid dominantly accounting for the difference. In line with this, high concentration of palmitate led to down regulation of C5 expression in goose primary hepatocytes whereas upregulation in mouse primary hepatocytes and HepG2 cells. In conclusion, regulation on C5 expression by fatty liver related factors including high level of palmitic acid may contribute to the protection of goose liver from severe hepatic steatosis.     

捕食线虫性真菌的分离培养及分布规律

对自然界中的捕食线虫性真菌进行了分离和种属区分，并对分布规律进行了研究。从土壤、粪便等材料中获得了2类活性较强的捕食线虫性菌株：即套捕型(hooping)捕食线虫性真菌和粘捕型(sticking)捕食线虫性真菌。主要的代表种类有3种。这些捕食线虫性真菌在捕食性结构分生孢子形态及某些生物学特性上有一定差异。套捕型捕食线虫性真菌代表种为少孢节丛孢菌(Arthrobotrys oligospara)和梨形指环菌(Dactylaria pyriformis)，它们以菌环、菌网作为捕食性结构(器官)，以套捕方式杀灭线虫幼虫。梨形指环菌可产生多量厚垣孢子(chlamyalospore)。粘捕型捕食线虫性真菌代表种为纺锤隔指孢菌(Dactylella ellipsospora)。可形成菌结捕食线虫性结构，以粘捕的方式杀灭线虫幼虫。结果表明：捕食线虫性真菌在土壤和家畜粪便中的分离率是40．41％；此类菌适合于生存在阴暗、潮湿、富含腐植质的环境中。在温暖季节时，采用0．4g／L玉米粉琼脂培养基(CMA)易于对其进行分离培养。     

樟子松枯梢病的防治研究

樟子松移植苗枯梢病Sphaeropsis sapinea可以通过药剂有效地进行防治。移植前以50％多菌灵wp500倍液浸苗；在人工林中，内吸性杀菌剂－甲基托布津、多菌灵为首选药剂；在树木新梢生长停止期和展叶中期（辽西北为6月初和6月中旬），70％甲基托布津wp 1000倍液或50％多菌灵wp 500倍液防治2次。保护性杀菌剂－百菌清效果不佳。适时的卫生伐对该病有明显的抑制作用。     

对江苏省发展木片生产的几点思考

论述了江苏发展木片生产的意义和现状，分析了存在的问题及有利条件，提出了８条切实可行的建议。     

狗枣猕猴桃果实软化过程中阶段性专一酶的研究

狗枣猕猴桃果实采后软化过程分两个阶段，第一阶段软化较快，起主要作用的阶段性专一酶是淀粉酶；第二阶段软化较慢，起主要作用的阶段性专一酶是多聚半乳糖醛酸酶。乙烯释放对果实软化有促进作用；保护性酶（过氧化物酶。过氧化氢酶）出现在果实软化后期，因而不是果实软化的阶段性专一酶。     

金秋橘飘香 黄岩采橘忙——中国蜜橘之乡采访记实

在一个秋风送爽的日子，伴随着秋风中飘动的甜甜橘香，我来到了位于浙江省东部沿海的台州市黄岩区。黄岩蜜橘的名字在小时候就听说过，是橘子中的魁首。这次来到黄岩，想到要与心仪已久的蜜橘谋面，心中就充满了期待和兴奋。     

麦田除草剂顶尖的应用技术研究

研究了15%顶尖可湿性粉剂在小麦田不同用药时间、不同剂量、不同对水量及混用对小麦田杂草的控制效果,探讨了顶尖在小麦田的使用技术。     

一类空间分解问题

讨论了一类与区域分解算法有关的空间分解问题，将二阶问题的已有的空间分解结果推广到四阶问题的相应空间中。还证明了一致重迭性条件与Ｂａｄｅａ条件的等价性。     

聚焦绿色建筑：揭开绿色建筑的面纱

2005年是中国“绿色地产年”，谈起绿色地产、绿色楼盘，不少人的头脑中立即就会反映出小区的绿化、花草树木与楼房的搭配和比例。把绿色理解成绿化的消费者不在少数．如果不是经过半个月的采访．就是记者本身也存在着相同的认识。“安得广厦千万间．大庇天下寒士俱欢颜”这是唐代大诗人杜甫对人需要居住的慨叹。时光过去了一千余年，人类仅需要遮风挡雨的草堂时代．早已成为了历史。现在人们不仅要求有房子住．而且要住的舒适惬意。而绿色建筑就是符合人们现代居住需求的房子。在2005“绿色地产年”接近尾声的时候．本刊记者用半个月的时间走访了相关的政府部门．建筑设计者、开发商以及消费者，对绿色建筑进行了一次全方位的调查。本期特别策划，我们汇集了记者、专家、开发商三方的见解，力图为读者立体地揭开绿色建筑的神秘面纱。