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Ricardo Tadeu de Faria Deonisio Destro João Carlos Bespalhok Filho Rolf Dieter Illg 《Euphytica》2002,124(1):59-63
The goal of this research was to study the introgression of the high regeneration capacity of Lycopersicon pimpinellifolium Mill line WV-700, in recalcitrant tomato cultivars (L. esculentum Mill cvs. Petomech, Santa Rita and VFN-8) using backcrossing. Hypocotyl explants of in vitro germinated seeds were cultivated in half strength MS medium supplemented with 5mg/l 6-BA to assess their shoot regeneration
capacity. The apical shoot of the in vitro germinated seeds were cultured on a separate medium. Apical shoots from genotypes showing high regeneration rates were acclimated
in a glasshouse and used as pollen donors for the next backcrossing. After four backcrossings, the material showed a similar
mean fruit weight for the cultivated tomato and a high regeneration capacity similar to the wild species. It is shown that
L. pimpinellifolium can be used with success as donor parent to introgress in vitro regeneration capacity to recalcitrant tomato cultivars.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
2.
Effects of concanavalin A on the progesterone production by bovine steroidogenic luteal cells in vitro
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FC Destro I Martin FDC Landim‐Alvarenga JCP Ferreira JL Pate 《Reproduction in domestic animals》2016,51(5):848-852
The aim of this study was to evaluate the effects of concanavalin A (CONA) on the progesterone (P4) production by bovine steroidogenic luteal cells (LCs) in vitro. Luteal cells were collected during the mid‐luteal stage (at 10–12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO2, with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 μg/ml); LH (100 μg/ml); CONA + LH; LH (100 μg/ml) + prostaglandin F2α (PGF2α) (10 ng/ml); CONA + LH + PGF2α. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p < .05. Culture in the presence of CONA decreased the P4‐secreting capacity of LCs on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments. 相似文献
3.
Nunes TP Martins CG Behrens JH Souza KL Genovese MI Destro MT Landgraf M 《Journal of agricultural and food chemistry》2008,56(4):1264-1268
This work studied the radiation resistance of Listeria monocytogenes and Salmonella species and the effect of irradiation on leaf flavonoid content and sensory acceptability of minimally processed arugula. Immersion in ozone-treated water reduced the analyzed microorganisms by 1 log. L. monocytogenes and Salmonella were not isolated from samples. Samples of this vegetable were inoculated with a cocktail of Salmonella spp. and L. monocytogenes and exposed to gamma irradiation. D10 values for Salmonella ranged from 0.16 to 0.19 kGy and for L. monocytogenes from 0.37 to 0.48 kGy. Kaempferol glycoside levels were 4 and ca. 3 times higher in samples exposed to 1 and 2 kGy, respectively, than in control samples. An increase in quercetin glycoside was also observed mainly in samples exposed to 1 kGy. In sensory evaluation, arugula had good acceptability, even after exposure to 2 and 4 kGy. These results indicate that irradiation has potential as a practical processing step to improve the safety of arugula. 相似文献
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Elvandro C.A. Lopes Deonisio Destro Ricardo Montalván Maurício U. Ventura Edson Perez Guerra 《Euphytica》1997,97(2):161-166
An experiment was carried out at the State University of Londrina School Farm in a randomized complete blocks design with
three replications; the plots underwent natural stink bug attacks to check correlations existing among agronomic traits and
stink bug damage in 33 F8 pure lines soybean. The seed size trait was measured by the weight of a hundred seeds (WHS) and the percentage of stink bug
damage seeds (PDS) was measured by staining the seeds with tetrazolium at 0.01%. Seeds were considered damaged with at least
one puncture and or with marks characteristic of stink bug. The phenotypic and genotypic correlations between the WHS and
the PDS seeds were positive, being 0.51 and 0.67 ± 0.19, respectively. The relationship between the visual pod width (VPW)
and PDS was also significant, being 0.41 and 0.54 ± 0.19 for phenotypic and genotypic correlations, respectively. Thus the
breeder can select lines for stink bug resistance indirectly either by WHS or VPW. These two traits are very easy to assess
when compared with the assessment of PDS damage, allowing the use of larger populations. However, in this case, the genetic
gains by selection were smaller than those obtained by direct selection, but even so they were considered noteworthy. The
VPW may be an efficient way of selection in large populations for stink bug resistance.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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