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1.
Arnulf Soleng Antonio B.S. Polo Tor A. Bakke 《Aquaculture (Amsterdam, Netherlands)》2005,250(3-4):616-620
Both laboratory and field experiments have demonstrated that aqueous aluminium can act as a paraciticide to the monogenean Gyrodactylus salaris Malmberg, 1957 infecting freshwater salmonids. However, the reproductive conditions of gyrodactylids surviving to cessation of an aluminium exposure is unknown. Therefore, Atlantic salmon (Salmo salar L.) parr infected with G. salaris was experimentally exposed to elevated concentrations of aqueous aluminium for more than 1 month. During this period, the infection increased the first week before it peaked and started to steadily decline approaching elimination. When almost all parasite specimens were eliminated, the water quality was adjusted to normal aluminium-poor freshwater. During the next 3 weeks, the surviving G. salaris were shown to clearly resume their reproduction. The experiment demonstrates the potential of the gyrodactylids to reproduce after cessation of an aluminium exposure. Thus, the as-yet unknown mechanism behind the toxic effects of aluminium on G. salaris survival and reproduction might to some extent be reversible. The present study gives further support for this metal as a parasiticide to G. salaris but demonstrates at the same time that in order to eliminate the parasites totally, the aluminium treatment must eradicate all G. salaris from the skin of the infected hosts. Furthermore, the potential development of resistance to aluminium should also be studied. 相似文献
2.
P. Nilsson Sven‐Åke Johansson Arnulf Merker 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):159-164
The seed oil composition and content in a number of accessions from species of the genera Barbarea and Lepidium were analysed. The oil from most accessions of B. verna contained more than 50% erucic acid, while the oil from B. vulgaris contained ~ 30% erucic acid, and 22% each of oleic and linoleic acid. The oil from B. intermedia resembled that of B. verna. The oil content was ~ 30% in all three species. The oil composition of L. campestre and L. heterophyllum is ~ 35% linolenic acid and 23% erucic acid. Other Lepidium species had up to 47% linolenic acid in the oil. The oil content of L. campestre and L. heterophylhim was just below 20%, but other species had more than 30% oil in their seeds. The variation in oil composition found within and between species is considered sufficient for the breeding of valuable oil qualities in both genera. 相似文献
3.
Schuler D Chevalier HJ Merker M Morgenthal K Ravanat JL Sagelsdorff P Walter M Weber K McGregor D 《Journal of toxicologic pathology》2011,24(3):149-162
Inhalation of vanadium pentoxide clearly increases the incidence of
alveolar/bronchiolar neoplasms in male and female B6C3F1 mice at all
concentrations tested (1, 2 or 4 mg/m3), whereas responses in F344/N
rats was, at most, ambiguous. While vanadium pentoxide is mutagenic in
vitro and possibly in vivo in mice, this does not
explain the species or site specificity of the neoplastic response. A nose-only
inhalation study was conducted in female B6C3F1 mice (0, 0.25, 1 and
4 mg/m3, 6 h/day for 16 days) to explore histopathological,
biochemical (α-tocopherol, glutathione and F2-isoprostane) and genetic (comet
assays and 9 specific DNA-oxo-adducts) changes in the lungs. No treatment
related histopathology was observed at 0.25 mg/m3. At 1 and
4 mg/m3, exposure-dependent increases were observed in lung
weight, alveolar histiocytosis, sub-acute alveolitis and/or granulocytic
infiltration and a generally time-dependent increased cell proliferation rate of
histiocytes. Glutathione was slightly increased, whereas there were no
consistent changes in α-tocopherol or 8-isoprostane F2α. There was no evidence
for DNA strand breakage in lung or BAL cells, but there was an increase in
8-oxodGuo DNA lesions that could have been due to vanadium pentoxide induction
of the lesions or inhibition of repair of spontaneous lesions. Thus, earlier
reports of histopathological changes in the lungs after inhalation of vanadium
pentoxide were confirmed, but no evidence has yet emerged for a genotoxic mode
of action. Evidence is weak for oxidative stress playing any role in lung
carcinogenesis at the lowest effective concentrations of vanadium pentoxide. 相似文献
4.
Carlos Henry Loáisiga Agnese Kolodinska Brantestam Oscar Diaz Björn Salomon Arnulf Merker 《Genetic Resources and Crop Evolution》2011,58(7):1021-1028
Teosintes are the closest relatives to modern maize, Zea mays L. ssp. mays. They are wild grasses with a native distribution area from Mexico to Nicaragua and represent an important genetic resource.
However, the genetic diversity of Nicaraguan teosinte (Zea nicaraguensis Iltis et Benz) has not yet been determined. This teosinte species has decreased in the last 25 years and now must be regarded
as an endangered species. An analysis of the genetic diversity of Zea nicaraguensis was carried out in a total of 240 individuals from seven populations. Eleven Simple Sequences Repeat (SSR) primer pairs were
used. A total of 42 alleles were found, the range of alleles per locus was 2–5 (mean 3.8) and the numbers of genotypes varied
between primers. The primer Bnlg 1538 showed the highest value, with 45 genotypes through all populations. The genetic diversity observed (Ho) between all
populations varied from 0.51 to 0.63, with an average of 0.563. One of the populations had as many as 40 alleles. Analysis
of molecular variance (AMOVA) revealed that most of the variation was within population, at a significantly high level (P < 0.001). Rare alleles were detected in all populations, but unique alleles were only found in four populations. These results
are highly relevant when developing conservation strategies and show that preserving populations in their natural habits is
highly important. 相似文献
5.
G. Belay E. Bechere D. Mitiku A. Merker S. Tsegaye 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(4):221-228
Thirty‐four tetraploid (2n = 4x = 28) wheat (Triticum turgidum L.) landrace populations collected from four regions in Ethiopia, and consisting of 4099 entries were characterized for glume colour, glume pubescence, beak awn, seed colour and spike density. The main objective was to analyse the diversity and distribution of these traits on the basis of administrative regions and altitudinal gradients. The Shannon‐Weaver Diversity Index (H') was used to estimate the magnitude of diversity. With the exception of spike density, all characters were polymorphic in all regions and most altitude groups. However, clinal patterns were observed in only a few cases. At the population level, the mean H’ varied from completely monomorphic (H’ = 0.00) to highly polymorphic (H’ = 0.80 ± 0.07). The highest diversity was found in regions with relatively better climatic conditions and in optimal altitude ranges. On country basis, seed colour (H’ = 0.98) and spike density (H’ = 0.43) displayed the highest and the lowest diversity indices, respectively. Spike density was the only character that exhibited significant differences (P<0.01) both “between regions”; and “between altitude groups”;. Across characters, most of the variance was due to the lowest hierarchy, the “within‐population”; component. The “between region”; and “between altitude group”; variances were significant, but spike density alone was responsible for the differences. The overall mean H’ for Ethiopia was 0.77 + 0.09 and did not seem to have reduced appreciably within the past 25 years or so, when compared to previous estimates. 相似文献
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A total of 4,840 entries of tetraploid wheat germplasm collections representing 121 accessions from Ethiopia were evaluated
for phenologic and qualitative trait diversity. The objectives were to assess the diversity patterns of the germplasm with
respect to regions, species and altitudinal class. High values of Shannon–Weaver Diversity Index (H′) were recorded for most
traits in each region, altitudinal classes, and species. Monomorphism was also high at accession levels. Both H′ and Nei’s
gene diversity value for the entire data set (total gene diversity H
T = 0.572; the within accessions gene diversity H
S = 0.112; and gene diversity among accessions D
ST = 0.460) showed the variation for the trait is mainly among accessions/populations rather than within accessions/population.
The least mean H′ value over all the traits used for the study was obtained from released varieties (among the origin groups)
and Triticum
dicoccon (among species). Triticum
durum exhibit the highest H′ for a number of traits. Accessions collected from altitudinal class III (2401–2800 m a.s.l.) and class
II (2001–2400 m a.s.l.) showed the highest H′ values for a valuable number of traits. Thus classifications using various phenology
and qualitative traits enable to identify adaptation of a genotype and would improve the evaluation of genotype for potential
adaptation. 相似文献
9.
Three tetraploid (2n= 4x= 28) wheat Triticum turgidum L. landrace morphotypes (= genotypes) from Ethiopia were found to carry a variant karyotype directly discernible under the microscope. This was possible because the rearrangement involved one of the satellited chromosomes. Giemsa C-banding revealed that the rearrangement resulted from a 5BS.6BS(5BL.6BL) centric reciprocal translation. The banding pattern on 5BL was polymorphic, suggesting that this translocation might have occurred more than once. There was little C-band polymorphism for the remaining chromosomes, except for 2A. As pure lines, all three morphotypes showed normal chromosome pairing at metaphase I (MI) in pollen mother cells (PMCs). indicating that they are genomically stable. Meiotic analyses of F1 hybrids and F2 segregates derived from crosses with tester varieties clearly indicated that one of them (B-l–9) carried another translocation. However, we were not successful in delecting the chromosomes involved, presumably the interchanged segments did nol include C-banding regions. By using T5BS.6BS, direct evidence for segregation distortion against translocation homozygotes in intervarietal hybrids was obtained. The distorted segregation was attributed lo zygotic selection. No aneuploid plants were obtained from the F2 segregates. However, translocation heterozygotes resulting in unstable meiosis were abundant in the F2 generation. The implications of the results in using the indigenous landraces in hybridization breeding are discussed. 相似文献
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