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采用平板纸片法测定药用植物刺五加内生放线菌菌株13-12发酵液抑菌活性及稳定性;PCR扩增、克隆并测序分析菌株活性物质合成相关功能基因;并通过生理生化、培养特征及16SrRNA进行分类鉴定。结果表明:菌株13-12发酵液对金黄色葡萄球菌、枯草芽孢杆菌、草分枝杆菌、MRSA均有不同程度的抑制作用,在4~20℃、pH6.0~8.0及光照48h条件下发酵液抑菌活性稳定;菌株基因组含有NRPS、Halo基因;通过菌株表型特征及分子特征初步鉴定该放线菌为游动单胞菌属(Planomonospora)有效发表种球形浮游动单胞菌(Planomonospora sphaerica)的一个菌株。菌株有合成β-内酰胺类抗生素等多种活性物质的潜能,具有进一步开发利用价值。 相似文献
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我国松树寄生线虫名录 总被引:1,自引:0,他引:1
目前我国松树寄生线虫有40个已知种和一些未定种,这些种分属于3目9科17属,该文从线虫分布和寄主两个方面列出名录. 相似文献
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赣南水土保持型果业发展模式探讨 总被引:8,自引:0,他引:8
针对江西赣南果业开发中普遍存在的严重水土流失、土地利用率低下、短期效益少等问题,采取以间、套种草、饲、经济作物为主要手段的水土保持措施,栽种“百喜草”,设置“山边沟”,引入美国籽粒苋发展养殖业,开展立体经营,形成果-苋-猪-沼-果良性循环。实现了果园综合效益的同步增长,达到了增加果园植被覆盖度,提高土地利用率,改善果园生态环境的目的。研究表明:果业开发中出现的严重水土流失现象不容忽视,因地制宜的水土流失治理措施与果园生态环境、经济效益密切相关。其综合经营的多种治理模式促进了各业的发展,取得了明显的生态、经 相似文献
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Comparative plasma and tissue pharmacokinetics and drug residue profiles of different chemotherapeutants in fowls and rabbits 总被引:1,自引:0,他引:1
T. LI G.L. QIAO G.Z. HU F.D. MENG Y.S. QIU X.Y. ZHANG W.X. GUO H.L. YIE S.F. LI S.Y. LI 《Journal of veterinary pharmacology and therapeutics》1995,18(4):260-273
Blood and tissue pharmacokinetics and drug residue profiles of six chemotherapeutants were studied. Ceftriaxone (CEF), intravenously at 50 mg/kg, sulfamonomethoxine (SMM) and sulfaquinoxaline (SQ), orally at 200 mg/kg, and olaquindox (OLA), orally at 50 mg/kg, were administered to young broilers. Penicillin (PEN), intramuscularly at 200 000 U/kg, and albendazole (ALB), orally at 20 mg/kg, were given to rabbits. For each drug, 13–18 groups ( n = 5–10 individuals/group) of the dosed animals were killed at different post-dosing times. Drug and/or metabolite concentrations in plasma, liver, kidney, heart, lung, and muscle tissues were analysed by HPLC procedures. Multi-exponential kinetic models were fitted to the observed tissue concentration-time data by applying a non-linear least-squares regression computer program. Tissue half-life, peak tissue concentration, and time of peak tissue concentration were determined. Half-life of CEF, SMM, SQ, OLA, PEN, ALB, and two metabolites of ALB (sulfoxide and sulfone) in various tissues ranged 0.6–1.4, 4.7–9.0, 4.5–18.9, 1.8–3.1, 0.9–3.0, 3.4–9.6, 5.0–16.1 and 7.4–12.2 h. The times required for CEF, SMM, SQ, OLA, PEN, and ALB residue concentrations to decline to 0.1 μg/g in various tissues ranged from 5.0–11.6. 70.0–110.5. 114.0–179.8, 21.3–30.3,4.1–24.8 and 47.8–84.4 h. Drug kinetic characteristics in tissues differed significantly from those in plasma, and also varied from tissue to tissue. It is necessary, therefore, to evaluate tissue kinetics when designing dosage regimens in tissue infection chemotherapy with these drugs. Knowledge of tissue kinetics is also important in predicting and controlling drug residues in edible tissues of food-producing animals. 相似文献
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试验以Cr2O3为指示物,测定草鱼对进口鱼粉、国产鱼粉、蟹粉、肉粉、肠衣粉、酵母、菜饼、黄菜饼、黑菜饼、双低菜仔粕、豆粕、膨化大豆、芝麻饼、棉粕、玉米胚芽饼、玉米蛋白粉和酒糟粉17种试验原料中粗蛋白和粗脂肪的表观消化率。试验饲料由基础饲料和试验原料以70:30的比例(质量比)混合挤压成硬颗粒饲料。结果表明,草鱼对鱼粉、菜籽饼籽、豆粕和膨化大豆等质量好的蛋白饲料的表观消化率较高、其干物质、脂肪和蛋白质的表观消化率均在80%以上;草鱼对酵母、菜籽饼粕、豆粕和膨化大豆等饲料的干物质、脂肪和蛋白质的表观消化率均与鱼粉一致。 相似文献
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