全文获取类型
收费全文 | 7885篇 |
免费 | 489篇 |
国内免费 | 902篇 |
专业分类
林业 | 763篇 |
农学 | 685篇 |
基础科学 | 618篇 |
1080篇 | |
综合类 | 3288篇 |
农作物 | 462篇 |
水产渔业 | 364篇 |
畜牧兽医 | 960篇 |
园艺 | 531篇 |
植物保护 | 525篇 |
出版年
2024年 | 60篇 |
2023年 | 174篇 |
2022年 | 425篇 |
2021年 | 412篇 |
2020年 | 422篇 |
2019年 | 411篇 |
2018年 | 309篇 |
2017年 | 427篇 |
2016年 | 373篇 |
2015年 | 469篇 |
2014年 | 456篇 |
2013年 | 542篇 |
2012年 | 760篇 |
2011年 | 712篇 |
2010年 | 596篇 |
2009年 | 552篇 |
2008年 | 459篇 |
2007年 | 423篇 |
2006年 | 289篇 |
2005年 | 236篇 |
2004年 | 121篇 |
2003年 | 102篇 |
2002年 | 78篇 |
2001年 | 86篇 |
2000年 | 74篇 |
1999年 | 57篇 |
1998年 | 58篇 |
1997年 | 32篇 |
1996年 | 27篇 |
1995年 | 23篇 |
1994年 | 22篇 |
1993年 | 25篇 |
1992年 | 20篇 |
1991年 | 5篇 |
1990年 | 9篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1965年 | 1篇 |
1964年 | 3篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1956年 | 5篇 |
1955年 | 1篇 |
排序方式: 共有9276条查询结果,搜索用时 0 毫秒
1.
2.
‘金美’是从云南野外美味猕猴桃资源中选育出的绿肉高糖新品种。果实为卵形,果面黄褐色硬毛,果形整齐;平均单果质量60 ~ 80 g。果肉绿色或黄绿色,质嫩多汁,风味浓甜。软熟果实含可溶性固形物24.1%,可溶性总糖16.2%,总酸1.25%,维生素C 1 180 mg • kg-1,钾 342 mg • kg-1,钙84.2 mg • kg-1。在武汉地区8月果实成熟,此时果实采后7 d开始软熟,1 ~ 2 ℃低温和95%相对湿度下可存放100 ~ 120 d。盛果期产量22 t • hm-2。 相似文献
3.
4.
5.
为了保护珍贵的十里香茶树资源,掌握利于十里香茶的最优组培条件,本研究对十里香茶组培过程中外植体类型、消毒时间、培养基添加物及培养条件进行了筛选。结果表明,采用轻微木质化的带腋芽茎段经升汞消毒15 min,接种后暗培养5 d,MS+0.2 mg·L^-1 NAA培养基中添加3 mg·L^-1 PVP+2000 mg·L^-1 Vc或者6 mg·L^-1 PVP+3000 mg·L^-1 AC,可在低污染率的前提下将褐化率降为10%左右,一定程度上解决了茶树组培中褐化率高的难题;采用1/8 MS+1 mg·L^-1 IBA培养基有利于外植体生根且增殖迅速。本研究建立了十里香茶的无菌苗离体培养及再生体系,可通过组培手段对其进行大量繁殖。 相似文献
6.
Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l−1) and kinetin (KT) (0, 0.1 or 0.2 mg l−1) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l−1 for ‘Bison’ and on medium containing 4 mg l−1 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l−1 2, 4-D plus 0.1 mg l−1 kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil. 相似文献
7.
AIM: To study the effect and mechanism of all-trans retinoic acid (atRA) on neural stem cell (NSCs) proliferation and differentiation from new born Sprague-Dawley rat striatum. METHODS: NSCs were isolated from the brains of new born Sprague-Dawley rat striatum, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on cell cycle distribution and proliferation of NSCs were determined by flow cytometry (FCM). The effects of atRA on differentiation of NSCs were determined by immunofluorescence staining and classified count of differentiated cells. RESULTS: FCM assay indicated that atRA inhibited the proliferation of NSCs. The percentage of cells in G0/G1 phase in atRA treatment group was significantly higher than that in control, and the proliferation index (PI) was significantly low. The percentage of neurons differentiated from NSCs in atRA group was 2.5 times of the control group after induced by adding 10% FCS in culture medium. CONCLUSION: atRA counteracts the effects of bFGF on the promotion of mitosis and inhibition of differentiation of NSCs. atRA also promotes NSCs to differentiate into neurons in vitro. 相似文献
8.
浅谈提高高寒山区大豆单产的途径 总被引:3,自引:0,他引:3
本文分析了限制黑河地区大豆单产提高的原因,其原因是盲目引种、密度不适宜、肥效降低、病虫危害严重及重迎茬问题。提出了提高高寒山区大豆单产的方法。 相似文献
9.
10.