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Shuhei ENJOJI Ryotaro YABE Nobuyuki FUJIWARA Shunya TSUJI Michael P. VITEK Takuya MIZUNO Takayuki NAKAGAWA Tatsuya USUI Takashi OHAMA Koichi SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1451-1456
Canine melanoma is one of the most important diseases in small animal medicine.
Protein phosphatase 2A (PP2A), a well conserved serine/threonine phosphatase, plays a
critical role as a tumor suppressor. SET/I2PP2A is an endogenous inhibitor for PP2A, which
directly binds to PP2A and suppresses its phosphatase activity. Elevated SET protein
levels have been reported to exacerbate human tumor progression. The role of SET in canine
melanoma, however, has not been understood. Here, we investigated the potential
therapeutic role for SET inhibitors in canine melanoma. The expression of SET protein was
observed in 6 canine melanoma cell lines. We used CMeC-1 cells (primary origin) and CMeC-2
cells (metastatic origin) to generate cell lines stably expressing SET-targeting shRNAs.
Knockdown of SET expression in CMeC-2, but not in CMeC-1, leads to decreased cell
proliferation, invasion and colony formation. Phosphorylation level of p70 S6 kinase was
decreased by SET knockdown in CMeC-2, suggesting the involvement of mTOR (mammalian target
of rapamycin)/p70 S6 kinase signaling. The SET inhibitors, OP449 and FTY720, more
effectively killed CMeC-2 than CMeC-1. We observed PP2A activation in CMeC-2 treated with
OP449 and FTY720. These results demonstrated the potential therapeutic application of SET
inhibitors for canine melanoma. 相似文献
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Khounsaknalath SITHYPHONE Mitsuyasu YABE Hiroshi HORITA Keisuke HAYASHI Tomiko FUMITA Yuji SHIOTSUKA Tetsuji ETOH Fumio EBARA Olavanh SAMADMANIVONG Jochen WEGNER Takafumi GOTOH 《Animal Science Journal》2011,82(2):352-359
The objective of this article is to compare feed cost, palatability and environmental impacts among feeding systems of high concentrate (HC), high hay (HH) and grass‐only‐fed (Gof) groups. Feed cost was the sum of costs paid for feed intake times the price of feed per kilogram. Palatability was measured by a panel taste test using HH and Gof beef and analyzed for differences. Environmental impacts were calculated based on 1 kg of Japanese beef yield of CO2 equivalents (eq) and animal end weights at each feeding stage. Results showed that the HH and Gof feeding systems could significantly reduce feed costs by approximately 60% and 78%, respectively, from the HC. In the panel taste test, 50% and 47.50% of panelists indicated that HH beef was ‘extremely delicious’ and ‘acceptable,’ respectively, while 15% indicated that Gof beef was ‘extremely delicious’; 62.50% indicated that Gof beef was ‘acceptable.’ Environmental impacts of each feeding system in terms of CO2 equivalents (eq) were 9.32, 6.10 and 2.04 tonnes of eq for the HC, HH and Gof, respectively. The HH was an economical system that produced moderate impacts on the environment and had impressive taste. 相似文献
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K. YOSHIDA K. YABE S. NISHIDA N. YAMAMOTO C. OHSHIMA M. SEKIGUCHI K. YAMADA & K. FURUHAMA 《Journal of veterinary pharmacology and therapeutics》1998,21(2):128-132
We examined the relation between the pharmacokinetic disposition and arthropathic potential of ofloxacin, a new quinolone antibacterial agent, using both male immature (3-month-old) and mature (18-month-old) beagles. Ofloxacin was orally administered to these dogs at 20 mg/kg once daily for 8 consecutive days, and the animals were killed 2 h after the last treatment. Serum ofloxacin concentrations were repeatedly measured on days 1 and 7 by use of high-performance liquid chromatography (HPLC), and pharmacokinetic parameters were calculated. In addition, on day 8, the drug concentrations in the joint synovial fluid and humeral and femoral condyles were measured. Clinico-pathological tests of blood and serum or histopathological examination of bone specimens were also performed. Arthropathy was macroscopically observed in the cartilage surface of all immature dogs, but not in mature dogs. There were, however, no noticeable differences in pharmacokinetic parameters between the two age groups of dogs or between single and 7-day treatments. In contrast to the occurrence of arthropathic lesions, the synovial fluid and condylar drug concentrations in immature dogs was equal to or lower than those in mature dogs, suggesting that the pharmacokinetic disposition of ofloxacin may not be essential for cartilage lesions. 相似文献
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Masato KINOSHITA Taijiro YABE Mitsutoshi KUBOTA Kazuharu TAKEUCHI Satoshi KUBOTA Haruhiko TOYOHARA Morihiko SAKAGUCHI 《Fisheries Science》2002,68(3):618-626
SUMMARY: Toughness is one of the most important elements that define the commercial value of the raw meat of fish. Degradation of the extracellular matrix is thought to be a cause of postmortem tenderization of fish meat. A previous study has suggested that this tenderization is caused mainly by metalloproteinases. The present study seeks to identify the proteinase(s) involved in tenderization; hence, cloned cDNA of two gelatinases from Japanese flounder, which showed high homology with mammalian matrix metalloproteinase (MMP)-2 and MMP-9, were designated as jfMMP-2 and jfMMP-9 , respectively. Northern blot analysis revealed that jfMMP-2 mRNA was expressed almost ubiquitously in adult tissues including the brain, muscle, gill, heart, gut, kidney, spleen, testis, and ovary. In contrast, the expression of jfMMP-9 mRNA was observed in those tissues which were abundant in blood cells, such as kidney, spleen, heart, and gill. Both recombinant proteins (jfMMP-2 and jfMMP-9) produced with the COS-7 cell system exhibited gelatin-degrading activity that was sensitive to 1,10-phenanthroline, a typical metalloproteinase inhibitor. 相似文献
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