土壤养分位与广义氧化还原电极电势

 根据广义氧化还原理论,给出了土壤溶液中NH₄⁺,P,Ca等养料成份的广义氧化还原电极电势。与养分位比较,用广义氧化还原电极电势来量度土壤养分有效度具有较多的优越性。     

岭西电网经济分析

本文对岭西电网的经济运行调整进行了分析、总结。并应用《岭西电网经济运行计算》系统的计算结果，提出了岭西电网的经济运行调整办法。     

大理州奶牛主要疫病及兽药残留监测研究

随着大理州奶牛数量的大幅增长,奶牛主要疫病和兽药残留的问题也日益凸显出来,尤其是奶牛结核病和布鲁氏杆菌病(以下简称布病)等严重危害人类身体健康的人畜共患病越来越受到社会各界和广大消费者的广泛关注。为全面摸清我州奶牛结核病、布病的流行和兽药残留情况,为各级政府制     

黄麻（新、引进）品种在闽南地区的产量（鉴定）试验

通过对中国农科院麻类所新选育的3个黄麻圆果种和12个国外引进的黄麻优异品种进行2002-2005年品种比较试验，结果表明：C90-2、C90-6、C89-6比对照种粤圆五号增产14．97％-19．34％，均达极显著水平。O-3、O-1、O-3（红茎）比对照种宽叶长果增产32．02％-34．21％，均达极显著水平。O-4（Ⅱ）比对照种增产12．28％达显著水平。C-1、C-5比对照种粤圆五号增产11．90％和7．82％。且主要经济性状优良，抗病力强。黄麻品种C90-2、C90-6、C89-6、O-3、O-1、O-3（红茎）、0-4（Ⅱ）、C-1和C-5适合于福建闽南麻区推广种植。     

Influences of chronic hypoxia on the gene expression of Kv1.3,Kv2.1 and Kv3.1 induced by acute hypoxia in PASMC of rats

AIM and METHODS: Total RNA was extracted from 6th rat subcultured pulmonary artery smooth muscle cells(PASMC) exposed to continual chronic hypoxia or normoxia and the effects of chronic hypoxia on the changes of Kv1.3,Kv2.1,Kv3.1 mRNA in cultured PASMC induced by acute hypoxia were studied by semiquantitative RT-PCR in vitro. RESULTS:①Kv1.3,Kv2.1,Kv3.1 genes were found to be expressed in PASMC of rats exposed either to hypoxia or normxia.②The expression of Kv2.1 and Kv3.1 in 6th subcultured of PASMC in normaxia group could be upregulated by exposure to acute hypoxia,the levels of Kv2.1 and Kv3.1 mRNA were significantly increased from 0.646±0.092, 0.782±0.104 to 1.059±0.134, 0.985±0.116,respectively (P<0.01,n=5). ③PASMC cultured continuously in chronic hypoxia for 6 subcultures and then exposed to normoxia for 12 h,thereafter the expression of Kv2.1 and Kv3.1 were downregulated by acute hypoxia for 6 hours.The level of Kv2.1 mRNA was significantly decreased from 1.008±0.117 to 0.649±0.097 (P<0.01,n=5). CONCLUSION:Kv2.1,Kv3.1 genes might be oxygen sensitive genes.Chronic hypoxia might change the response of these Kv genes of PASMC to acute hypoxia and down-regulate its expression,which might probably decrease the role of Kv in HPV.     

Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction

AIM: To determine the role of Kv1.2, Kv1.5, Kv2.1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1.2/Kv1.5/Kv2.1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells . IKv of PASMC was decreased after 24 h hypoxia, . ② The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on them. ③ The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies and the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1.2, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated HPV.     

Effects of blocking the expression of PKARⅠβ gene with antisense nucleotide on Shuang long-Jie gu Pill (SLJGP) containing serum osteoblast proliferation

AIM: To further investigate the role of PKARⅠβ in the growth-promoting effects of shuang long Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA- antiPKARⅠβ, a recombinant expressing the antisense sequence of PKARⅠβ, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A.     

致麻鸭产蛋下降的副粘病毒的分离和鉴定

从浙江省某麻鸭养殖基地产蛋锐减的病鸭生殖器官分离到1株致产蛋下降、不致鸭死亡的病毒株(YH99V)。该病毒易感蛋鸭，经SPF鸡胚传至第9代时致病性突然增强，第11代出现对鸡红细胞的血凝特性，通常在42～80h致死SPF鸡胚，EF15EID50为10^1.8。经磷钨酸负染电镜观察，YH99V粒子呈现圆形、杆状形、葫芦状形等多形态，直径70～400nm不等，病毒外表有囊膜，囊膜外层有排列整齐的纤突。病毒粒子和包涵体位于细胞浆内。病毒抵抗力由弱到强依次为：0．2%甲醛、24h-56℃、45min→紫外线、1h→乙醚≈氯仿≈37℃、16h—pH9→pH5→1％Try。对人眼结膜易感，鸭眼结膜不易感。接种传代细胞BHK-21、IBRS-2均能引起细胞病变。YH99V株与同样引起鸭产蛋下降的AIV、鸭源EDSV无抗原相关性，而能被禽副粘病毒Ⅰ型阳性血清中和。根据试验结果，初步将YH99V判定为鸭副粘病毒。     

枸骨叶总黄酮提取与测定

以芦丁为对照品,乙醇为浸提溶剂,利用紫外-分光光度法对枸骨叶总黄酮提取率进行测定。在单因素实验基础上采用L9(34)正交设计,考察了乙醇浓度、浸提时间、浸提温度、料液比等因素对枸骨叶总黄酮提取率的影响。研究结果表明,在浸提温度为80℃、料液比为1∶12的φ乙醇=20%中浸提3 h的条件下浸提效果最好,枸骨叶总黄酮提取率为3.975%,浸提级数为二级。