不同稀释液对藏獒精液保存效果的影响

比较了8种稀释液在藏獒精液冷冻保存和低、常温保存(5 ℃,10 ℃,15 ℃,20 ℃)的效果.结果表明:2号和8号稀释液在冷冻保存时效果最好,精子活力和顶体完整率分别达到0.40和49%,与对照组相比差异显著(P<0.05).在藏獒的低温、常温保存试验中,所有稀释液在15 ℃时保存时间最长,综合考虑,7号液保存效果最好;在15℃时精子平均存活时间为103 h,显著高于对照组和其它稀释液(P<0.05).     

新的信息资源环境下图书馆员角色转变的思考

本文分析了信息时代图书馆员角色转变的几个重要因素及特点,揭示了新时代图书馆员应承担的几种重要角色,对信息时代图书馆员的发展进行了探讨.     

抓具抓取轨迹曲线及其绘制

在设计和研究抓具系统的功能和各种特性时,首先必须对抓具颚爪的抓取曲线进行研究。本文分析和论述了抓具的抓取轨迹曲线、影响抓取轨迹曲线的因素以及抓取曲线的绘制。     

对农业推广的信息服务

本文论述了农业信息服务与农业推广的相互关系，分析了图书馆在农业推广的农业信息服务工作中的作用和功能，并提出了相关的一些构想。     

能源甘蔗的开发利用

本文介绍国内外能源甘蔗育种现状，慨述能源甘蔗在能源、糖料、饲料等方面的利用及其副产品的综合开发利用，说明能源甘蔗作为一种新型的生物能源，具有广阔的发展前景。     

国产MJ3210小型木工跑车带锯机锯解正交试验分析

采用正交试验设计法，对国产MJ3210小型木工跑车带锯机的锯解质量进行研究。通过实测马尾松锯材尺寸偏差、表面粗糙度等参数，得到其最佳的进料速度、锯解厚度和锯卡高度的组合方案。结果表明，该设备具有结构简单、出材率高、锯解精度高、质量好、成本低和生产效率高等优点。     

CHANGE OF SURFACE CHARACTERISTICS DURINGOXYGEN BLEACHING OF SULPHATE PULP

INTRODUCTIONAtpresent,oxygenbleachinghasbecomeanlndustrializedtechnolOgy.Uptol989,thistechnologyhadbeenusedin58paPerndlls,and50%ofthemwereinEuroPe,20%inNorthemAmencaand20%inJapan.Inrecentyears,severaloxygnbleachiofequiPmenthadbeenintroducedintoChina.Inaword,oxygenbleachinglsasuccessfulandenviron-mentallyaccePtableprocessfOrPulpbleaching.However,littleworkhadbeendevotedtounderstandingthefundamentalaspectsofthissysten1,sotherewaslittledetailedinfOrmationonthel11echanismsofoxygenbleachi…     

供水工程影响下中国北方地区耕地后备资源开发潜力

灌溉水源是北方地区耕地后备资源能否有效开发的关键。当前,中国正在推动国家水网工程规划建设,为北方地区耕地后备资源开发提供了新的机遇。在以往研究基础上,该研究考虑北方地区已建、在建及规划的供水工程,基于自然适宜、利用高效、发展稳定三方面评价准则,结合三维魔方空间分类方法,开展了雨养情景和供水灌溉情景下耕地后备资源开发潜力分析。结果表明：雨养农业情景下,北方地区勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积分别为2.9万、1.0万和0.6万km²。灌溉农业情景下,耕地后备资源面积明显增加,勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积将分别达到5.4万、7.6万和6.5万km²,主要集中于新疆、内蒙古和甘肃3省（自治区）。该研究结果可为中国耕地后备资源开发利用及国家水网工程效益发挥提供参考。     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.