沙冬青GATA型锌指蛋白基因序列及表达分析

根据沙冬青低温干旱诱导cDNA文库表达序列标签中锌指蛋白基因序列设计特异引物,采用PCR技术扩增锌指蛋白基因AmZFPG的cDNA全长.序列分析表明,cDNA全长669 bp,推测编码223个氨基酸残基、分子量约25 kD的蛋白质.氨基酸保守性分析及同类锌指结构比对表明AmZFPG属于GATA结合蛋白家族,且具有高度的...     

Functional analysis of cold-inducible cDNA clones in the legume Ammopiptanthus mongolicus

Functional analysis of cold-inducible cDNA clones in the legume Ammopiptanthus mongolicus     

沙冬青A20／ANl锌指蛋白基因序列及表达分析

A20/AN1锌指蛋白普遍存在于真核生物中,在胁迫响应引起的信号传导过程中发挥着重要作用。本文利用生物信息学和RT-PCR方法从低温干旱胁迫的沙冬青叶片组织中分离锌指蛋白基因AmSTZF,该基因编码一条含172个氨基酸残基的多肽,含A20和AN1两个锌指蛋白保守结构域,属A20/AN1锌指蛋白。生物信息学分析显示该蛋白定位于细胞质中,具有磷酸化位点,参与细胞转录调控。RT PCR分析表明:低温和干旱胁迫处理的沙冬青叶片中,AmSTZF的表达增强,初步推测该基因与沙冬青低温、干旱胁迫响应相关。      

北京地区早熟禾属植物种质资源研究

为收集优良的草坪种质资源,对北京地区野生早熟禾属(Poa L.)植物资源进行了实地考察。北京共有早熟禾属植物20种1个亚种,其中7个种及1个亚种为新分布,西部和北部山区早熟禾属植物物种分布较为丰富,尤其是东灵山、百花山、海坨山及喇叭沟门。从垂直分布看,草地早熟禾(Poa pratensis L.)为广布种,从平原到高海拔地区均有分布。硬质早熟禾(Poasphondylodes Trin.)在低山区常见,其他物种主要分布在海拔1000 m以上的地区。通过研究23份来自不同居群的草地早熟禾花序特征,发现不同居群的花序性状间存在较大的变异,花序基部最短分枝长的变异最大,花序小穗数次之。     

4种不同种源小桐子种子发芽试验

对产于元谋、禄劝、双柏、攀枝花的4个小桐子种源用ABT3、NAA、IAA和GA 4种激素不同浓度处理进行室内和场圃发芽实验.结果表明:种源对种子发芽率和发芽势有显著影响,处理A3B2C4即元谋-GA-100×10-6的发芽率和发芽势最高,分别为80.0%和71.1%,场圃发芽率和发芽势为84.4%和71.1%;处理A3B1C3即元谋-ABT3-50×10-6的发芽速最快,为13.90 d,场圃发芽速为25.88 d.     

西藏山南地区栽培草地的现状与发展

西藏山南地区地处西藏腹地,农牧业交错,宽阔、平缓的河谷农田灌溉便利、土质肥沃,是西藏的粮食主产区,也是西藏栽培草地建设的主要地区.西藏20世纪70年代开始发展栽培草地,2009年栽培草地面积3.3万hm2,其中山南地区种草8 400 hm2,占全区种草总面积的25.5%.为提高冬季饲草储量,近年来,通过调整种植结构、种...     

Cold acclimation induced accumulation of phenolic compounds and freezing tolerance in Ammopiptanthus mongolicus

Ammopiptanthus mongolicus, the only freezing tolerant evergreen broad-leaved shrub, local species of the Alashan desert, northwest sand area of China, can survive -30°C or even lower temperature in winter. In the present study, the secondary products phenolics in A. mongolicus cotyledons were determined to study the effects of phenolics on cold tolerance. Cytochemical localization of phenolics in cotyledon cells was observed by electron microscopy and the content of phenolic compounds was assayed by spec-trophotometric measurement. The results showed that the freezing tolerance of A. mongolicus seedlings increased after acclimation at 2-6°C for 14 days, which accompanied the increase of the content of phenolic compounds in cotyledons. Cytochemical observation showed that phenolic deposits were mainly localized in vacuoles and in close proximity to tonoplast, and also in the cytoplasm. The amount and the size of phenolics droplets increased obviously in cytoplasm and vacuoles after cold acclimation, predominantly ag-gregated along membranes of vacuoles and tonoplast. No phenolic deposits were found in cell walls. As hydrogen-or elec-tron-donating agents, phenolics may protect plant cells against reactive oxygen species formed during chilling or freezing stress and improve the freezing tolerance of cold-acclimated A. mongolicus seedlings.     

对江苏林地规划问题的立法思考与建议

长期以来,江苏事实林地与规划林地数量之间存在较大的差距。作者提出应该从立法入手,转变思想,提高认识,理顺体制,切实履职,将江苏林地保护利用规划落到实处的建议。     

Effect of NaCl on growth and ion relations in two salt-tolerant strains of Paxillus involutus

The effect of NaCl on growth, biomass and ion relations of two salt-tolerant isolates of Paxillus involutus, MAJ and NAU were investigated. The two Paxillus strains were exposed to the following concentrations of NaCl： 0, 100, 200 and 500 mmol·L^-1. Growth of MAJ and NAU was enhanced by 100 mmol·L^-1 NaCl but severely inhibited at the concentration of 500 mmol·L^-1. NAU exhibited a greater capacity to exclude Na^＋ and Cl^- under all salinity levels, whereas the salt-includer MAJ had a higher capacity in nutrient uptake under salt stress. The ratios Na^＋/K^＋, Na^＋/Ca^2＋ and Na^＋/Mg^2＋ in NaCl-treated MAJ and NAU did not increase at levels of 100 and 200 mmol·L^-1 NaCl but markedly increased at 500 mmol·L^-1. This suggests that the two strains, especially MAJ, enhanced nutrient uptake corresponding to the increased Na^＋ influx at moderate salinity. We conclude that both MAJ and NAU are able to tolerate 500 mmol·L^-1 NaCl but there are species-specific differences in retaining ionic homeostasis in the two Paxillus strains. NAU is a salt-excluder, MAJ is a salt-includer but retains a high capacity in nutrient selectivity under saline conditions. Their definite mechanisms to enhance salt tolerance of mycorrhizal hosts need further study.     

3个居群野生草地早熟禾耐盐性比较研究

试验以山西3个不同居群野生草地早熟禾（HY居群、YX居群、QS居群）为材料,采用不同浓度的NaCl溶液（0、50 mmol·L^-1、100 mmol·L^-1、150 mmol·L^-1、200 mmol·L^-1、250 mmol·L^-1、300 mmol·L^-1）胁迫处理26 d后,测定叶片相对含水量（RWC）、质膜相对透性、超氧化物歧化酶（SOD）活性、丙二醛（MDA）、脯氨酸（Pro）、可溶性糖（SS）、可溶性蛋白（SP）含量以及光合参数,并采用隶属函数法综合分析3个野生优异居群的耐盐性。结果表明：随NaCl胁迫浓度的升高,HY居群、YX居群和QS居群的RWC和SP含量均呈下降趋势;质膜相对透性、MDA含量、Pro含量均呈升高趋势;SOD活性和SS含量在YX居群、QS居群中均呈先升高后降低趋势,而HY居群则呈持续升高趋势。3个居群的净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）均随胁迫浓度的升高呈下降趋势;胞间CO₂浓度（Ci）在0~150 mmol·L^-1NaCl胁迫下随盐浓度的升高而降低,但在200~300 mmol·L^-1NaCl胁迫下随盐浓度的升高而升高。3个居群草地早熟禾的耐盐性：HY居群 > YX居群 > QS居群。