Surveillance of West Nile virus in horses in Canada: A retrospective study of cases reported to the Canadian Food Inspection Agency from 2003 to 2019

The objectives of the study were to describe the regional and provincial incidence rates and the weekly distribution of 842 reported West Nile virus (WNV) cases in horses in Canada between 2003 and 2019. This study also investigated characteristics of cases reported to the Canadian Food Inspection Agency (CFIA) between 2015 and 2019. The western region (British Columbia, Alberta, Saskatchewan, and Manitoba) had higher incidence rates than the eastern region (Ontario, Quebec, and Atlantic provinces) and overall, Saskatchewan registered the highest incidence. Over the study period, an earlier weekly preliminary onset of WNV cases was observed in the western region. The vast majority of cases were unvaccinated (96%), most cases were Quarter Horses (68%) and the risk of mortality was 31.9%. The findings of this study may be useful in informing veterinary equine practitioners about measures to prevent WNV disease in horses in Canada.     

Effect of feed restriction on dairy cow milk production: a review

In the dairy cow, negative energy balance affects milk yield and composition as well as animal health. Studying the effects of negative energy balance on dairy cow milk production is thus essential. Feed restriction (FR) experiments attempting to reproduce negative energy balance by reducing the quantity or quality of the diet were conducted in order to better describe the animal physiology changes. The study of FR is also of interest since with climate change issues, cows may be increasingly faced with periods of drought leading to a shortage of forages. The aim of this article is to review the effects of FR during lactation in dairy cows to obtain a better understanding of metabolism changes and how it affects mammary gland activity and milk production and composition. A total of 41 papers studying FR in lactating cows were used to investigate physiological changes induced by these protocols. FR protocols affect the entire animal metabolism as indicated by changes in blood metabolites such as a decrease in glucose concentration and an increase in non-esterified fatty acid or β-hydroxybutyrate concentrations; hormonal regulations such as a decrease in insulin and insulin-like growth factor I or an increase in growth hormone concentrations. These variations indicated a mobilization of body reserve in most studies. FR also affects mammary gland activity through changes in gene expression and could affect mammary cell turnover through cell apoptosis, cell proliferation, and exfoliation of mammary epithelial cells into milk. Because of modifications of the mammary gland and general metabolism, FR decreases milk production and can affect milk composition with decreased lactose and protein concentrations and increased fat concentration. These effects, however, can vary widely depending on the type of restriction, its duration and intensity, or the stage of lactation in which it takes place. Finally, to avoid yield loss and metabolic disorders, it is important to identify reliable biomarkers to monitor energy balance.     

A 13CO2 Enrichment Experiment to Study the Synthesis Pathways of Polyunsaturated Fatty Acids of the Haptophyte Tisochrysis lutea

The production of polyunsaturated fatty acids (PUFA) in Tisochrysis lutea was studied using the gradual incorporation of a ¹³C-enriched isotopic marker, ¹³CO₂, for 24 h during the exponential growth of the algae. The ¹³C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in T. lutea. The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in ¹³C. On the contrary, 18:4n-3 and 18:5n-3 were the least enriched in ¹³C after long chain polyunsaturated fatty acids such as 20:5n-3 or 22:5n-3. The algae appeared to use different routes in parallel to form its polyunsaturated fatty acids. The use of the PKS pathway was hypothesized for polyunsaturated fatty acids with n-6 configuration (such as 22:5n-6) but might also exist for n-3 PUFA (especially 20:5n-3). With regard to the conventional n-3 PUFA pathway, Δ6 desaturation of 18:3n-3 appeared to be the most limiting step for T. lutea, “stopping” at the synthesis of 18:4n-3 and 18:5n-3. These two fatty acids were hypothesized to not undergo any further reaction of elongation and desaturation after being formed and were therefore considered “end-products”. To circumvent this limiting synthetic route, Tisochrysis lutea seemed to have developed an alternative route via Δ8 desaturation to produce longer chain fatty acids such as 20:5n-3 and 22:5n-3. 22:6n-3 presented a lower enrichment and appeared to be produced by a combination of different pathways: the conventional n-3 PUFA pathway by desaturation of 22:5n-3, the alternative route of ω-3 desaturase using 22:5n-6 as precursor, and possibly the PKS pathway. In this study, PKS synthesis looked particularly effective for producing long chain polyunsaturated fatty acids. The rate of enrichment of these compounds hypothetically synthesized by PKS is remarkably fast, making undetectable the ¹³C incorporation into their precursors. Finally, we identified a protein cluster gathering PKS sequences of proteins that are hypothesized allowing n-3 PUFA synthesis.     

Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations

Background  

The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as a model for berry-fruit plants. With the progress of the tomato sequencing project, reverse genetics becomes an obvious and achievable goal.     

Effect of glyphosate on symbiotic N2 fixation and nickel concentration in glyphosate-resistant soybeans

Decreased biological nitrogen fixation in glyphosate-resistant (GR) soybeans has been attributed directly to toxicity of glyphosate or its metabolites, to N₂-fixing microorganisms. As a strong metal chelator, glyphosate could influence symbiotic N₂ fixation by lowering the concentration of nickel (Ni) that is essential for the symbiotic microorganisms. Evaluation of different cultivars grown on different soil types at the State University of Maringá, PR, Brazil during the summer 2008 revealed, significant decreases in photosynthetic parameters (chlorophyll, photosynthetic rate, transpiration and stomatal conductance) and nickel content with glyphosate use (single or sequential application). This work demonstrated that glyphosate can influence the symbiotic N₂ fixation by lowering nickel content available to the symbiotic microorganisms.     

Vertical variations in wood basic density for two softwood species

European Journal of Forest Research - Studies on wood basic density (BD) vertical variations become essential to predict more accurately the within-stem distributions of biomass and wood quality in...     

New histochemical and ultrastructural observations on normal bovine tonsils.

Samples of normal bovine palatine tonsils were examined by light and electron microscopy. Like human tonsils, they were composed of crypts, subepithelial areas, follicles, and T-dependent zones, but their well-developed capsule subdivided the lymphoid tissue by connective septa. B cells formed the major lymphoid component. The follicles and T-dependent zones had morphological and histochemical features typical of peripheral lymph organs. Follicular dendritic cells were isolated and shown to be similar to human follicular dendritic cells.     

Developing weed-suppressive soils through improved soil quality management

Manipulating soil microbial communities using soil and crop management practices is a basic strategy in developing sustainable agricultural systems. Sustainable farming is based, in part, on the efficient management of soil microorganisms to improve soil quality. However, the identification of biological indicators of soil quality that can be used to predict weed suppression in soils has received little attention. We investigated differences in soil microbial activity among various crop and soil management systems to assess: (i) the microbiological characteristics of these soils; (ii) determine whether any relationships existed that might be used in the development of weed suppression. Soil enzyme activity, water-stable aggregates, and the proportions of weed-suppressive bacteria were compared among seven cropping systems and one native-prairie ecosystem in mid-Missouri, USA. Assays of soil enzymes (fluorescein diacetate hydrolase, dehydrogenase, phosphatase) revealed that organic and integrated cropping systems, and the native-prairie ecosystem had the highest levels of soil activity. Weed rhizospheres from these same ecosystems also had greater proportions of bacterial isolates characterized as “growth suppressive” to green foxtail (Setaria viridis [L.] Beauv.) and field bindweed (Convolvulus arvensis L.): 15 and 10%, respectively. The proportion of water-stable soil aggregates was the greatest in soils with the highest organic matter and was found to be related to higher enzyme and weed-suppressive activity. Selected biological indicators of soil quality were associated with potential weed-suppressive activity in soil when that soil was managed for high organic matter content under reduced tillage systems. This research study provides further evidence that soil quality and sustainable agricultural practices may be linked to integrated weed management systems for the biological suppression of weeds.     

Defining the poor by the rural communities of Burkina Faso: implications for the development of sustainable parkland management

Parklands are mixtures of trees and shrubs that farmers select for certain functions. In the Sahel, parklands are cultivated together with staple food crops, such as millet and sorghum. Parkland trees are sources of foods, including fruits, fats, oils, leafy vegetables, nuts and condiments that complement food crops in the local diet. Despite their socio-economic and ecological role, many studies have shown that the parklands are degrading very rapidly. Therefore, there is a need to undertake restoration actions that are based on a clear understanding of the livelihood context, such as the wealth status of the farmers, in which these agroecosystems are evolving. Thus, we conducted a wealth survey with rural communities in two different farming systems of Burkina Faso that are the cereal-based system in the “Plateau Central” and the cotton-based system in the “Boucle du Mouhoun”. A total of twelve villages were sampled, six villages for each system. The Participatory Analysis of Poverty and Livelihood Dynamics (PAPoLD) method was used by rural farmers to rank farmer households of their communities according to their wealth status. The results revealed that 70% of households in the Plateau Central and 56% in the “Boucle du Mouhoun” managed to escape poverty, and became wealthy. However, 2% of households of the villages in the Plateau Central and 6% in “Boucle du Mouhoun” fell into poverty in the same period whereas 9 and 12% remained poor in these zones, respectively. The main causes associated with households getting out of poverty are subsidies for cotton, external revenues from relatives working in towns and gardening activities. Death of spouse, illness, high number of people in the household and indebtedness are the main causes that lead into poverty. The implications of these findings in designing appropriate policies and management options for a sustainable management of agroforestry parklands under different land use systems were finally discussed.