排序方式: 共有23条查询结果,搜索用时 15 毫秒
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Elisabete Yurie Sataque Ono Elaine Cunha Moreno Mario Augusto Ono Carolina Nachi Rossi Gervásio Hitoshi Saito Édio Vizoni Yoshitsugu Sugiura Elisa Yoko Hirooka 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(4):653-660
In this study the effect of different cropping systems and crop successions was evaluated on natural Fusarium sp. contamination and fumonisin levels in corn. The cropping systems consisted of a conventional and no-tillage area cultivated
with corn in the summer following either oats or fallow in the winter (2006 and 2007 growing seasons). In addition, the effect
of applying nitrogen fertilizer (0, 22.5, 45.0, 90.0 and 90.0 kg ha−1 nitrogen supplemented with potassium oxide) on fumonisin contamination was evaluated in the 2006 growing season. Fusarium sp. was detected in 90% samples in 2006 and in 100% samples in 2007. In both growing seasons, no-till corn following oats
showed the highest mean fumonisin levels and differed significantly (P < 0.05) from all the others (2006) and from conventional till corn following either oats or fallow in the winter (2007).
Fumonisin levels ranged from 0.13 to 19.52 μg g−1 (mean 6.97 μg g−1) and from 3.70 to 7.75 μg g−1 (mean 6.29 μg g−1) in no-till corn following oats from the 2006 and 2007 growing seasons, respectively. Plots treated with 0 kg ha−1 and 22.5 kg ha−1 nitrogen showed the highest mean fumonisin levels and differed significantly from those with 45.0 and 90 kg ha−1 nitrogen. Fumonisin levels correlated negatively (P < 0.05) with the nitrogen fertilization rates. Although no-till is advantageous from a soil conservation standpoint, it may
enhance the potential for fumonisin contamination in corn. 相似文献
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Hosaka Y Sakamoto Y Kirisawa R Watanabe T Ueda H Takehana K Yamaguchi M 《The Japanese journal of veterinary research》2004,52(3):135-144
Although tumor necrosis factor (TNF) alpha is an important key factor in degeneration of equine superficial digital flexor tendon (SDFT), the dynamism of TNF receptors and associated factors on tendinocytes has not been elucidated. To reveal signaling events mediated by TNF-receptors (TNF-Rs) in tendinocytes, we focused on four signaling factors, TNF-R1, TNF-R2, TNF-R-associated factor 2 (TRAF2) and nuclear factor-kappa B (NF-kappaB), and investigated the distribution and production of these factors. Cultured tendinocytes were obtained from SDFTs of thoroughbred horses. The tendinocytes were treated with 10 ng/ml equine TNFalpha medium for 6 hours and then the four factors on tendinocytes were visualized by using an immunohistochemical method, and the amounts of the four factors were determined by Western blot analysis. Although TNF-R1 and TNF-R2 co-localized on the same tendinocyte, in untreated control cells (normal condition), immunoreactivity against TNF -R1 was very weak but TNF-R2 showed a strong reaction. However, TNF-R1 showed the same high level of reaction as TNF-R2 in TNFalpha-treated cells (inflamed condition). Intense TRAF2 and NF-kappaB were detected at inflamed condition, however both factors were also detected at normal condition. The distinct distributions of the four factors under different conditions (normal and inflamed condition) in vitro not only reflect the dynamism of the cytokines but may also provide important clues for a means to prevent from occurrence of tendonitis and progress of tendon degeneration. 相似文献
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Hideaki Hayashi Chigusa Arai Yurie Ikeuchi Maho Yamanaka Takuji Hirayama 《Animal Science Journal》2021,92(1):e13518
The effect of growth and parturition on hair cortisol concentrations of cattle was investigated. Plasma, saliva, and hair (black and white from the shoulders and hip) samples were collected from calves at 6 and 24 weeks old and from dairy cattle at the dry (1 and 2 months prepartum) and lactation (10, 50, 150, and 250 days postpartum) periods. Plasma and saliva cortisol concentrations were lower in 24-week-old calves than those of 6-week-old calves, and hair cortisol concentrations decreased regardless of color and position. In 6-week-old calves, hair cortisol concentrations differed between sampling positions, but this difference was not observed in 24-week-old calves. Plasma and saliva cortisol concentrations increased before parturition until 10 days postpartum then decreased until 50 days postpartum. The same trend was observed in the cortisol concentrations of white hair. Contrarily, cortisol concentrations in black hair remained unchanged and was lower than that in white hair. Hair cortisol concentration can vary greatly depending on the location on the body, hair color, cattle age, or parturition. When this method is used, all of the above factors must be considered. 相似文献
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Kiri Hayakawa Takayuki Sakamoto Atsushi Ishii Keita Yamaji Yoshinobu Uemoto Nanae Sasago Eiji Kobayashi Naohiko Kobayashi Tamako Matsuhashi Shin Maruyama Hirokazu Matsumoto Kenji Oyama Hideyuki Mannen Shinji Sasazaki 《Animal Science Journal》2015,86(8):737-746
The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non‐synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome‐wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome‐wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high‐grade beef as a selection marker in beef cattle. 相似文献
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Takashi Tsukamoto Hiroshi Uchida Hiromi Nakanishi Shingo Nishiyama Hideo Tsukada Shinpei Matsuhashi 《Soil Science and Plant Nutrition》2013,59(7):1085-1088
5-Aminolevulinic acid (ALA) acts to increase chlorophyll biosynthesis, photosynthesis, cold stress tolerance, and salt tolerance at low concentrations. We studied the effects of ALA on H2 15O translocation from the roots to the shoots of rice plants (Oryza sativa L. cv. Nipponbare) in real time by a positron-emitting tracer imaging system (PETIS). When the plant was treated with 10 μm ALA, the velocity of the H2 15O translocation from 2 to 12 min after absorption increased to 126, 137, 140% that of the control at 1.5, 2.5, and 3.5 h after ALA treatment, respectively. However, ALA did not affect the H2 15O translocation within 0.5 h of treatment. When the plant was treated with 0.1 mM ABA at 4 h after 10 μm ALA treatment, the velocity of the H2 15O translocation decreased at 0.5 h after ABA treatment. Those observations suggested ALA might be absorbed and transported to the guard cells within 1.5 h and functioned to expand the stomatal aperture. 相似文献
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Yurie KAWASAKI Yuka AOKI Fumie MAGATA Akio MIYAMOTO Chiho KAWASHIMA Takuo HOJO Kiyoshi OKUDA Koumei SHIRASUNA Takashi SHIMIZU 《The Journal of reproduction and development》2014,60(3):173-178
The present study aimed to assess the effect of polymorphisms in the tumor necrosis factor α (TNF-α) promoter (A/A, A/G and
G/G) and exons (T/T, T/C and C/C) on immune function and reproductive performance in dairy cows. The occurrence of the first
postpartum ovulation within 3 weeks in the cows with the TNF-α promoter A/G and G/G genotypes was higher than in the A/A
group. Among the different TNF-α exon genotypes, the occurrence of early first postpartum ovulation was higher in the T/C and
C/C genotype groups than in the T/T group. Single nucleotide polymorphisms (SNPs) in the TNF-α gene did not affect the rate
of artificial insemination (AI) or duration from parturition to next conception (days open). The apoptosis rate of
polymorphonuclear leukocytes (PMNs) did not differ among the TNF-α promoter genotypes, but the PMN transmigration rate was
significantly higher for the A/A and A/G genotypes than for the G/G genotype. Interleukin 8 (IL-8) mRNA expression in PMNs
and peripheral blood mononuclear cells (PBMCs) before culture was significantly higher for the A/A genotype compared with the
G/G genotype. There were no significant differences between the genotypes in the mRNA expression of TNF-α, IL-6, IL-1β, and
toll-like receptor 4 (TLR4) in PMNs and PBMCs before and 4 h after culture. IL-8 and IL-1β production by PBMCs cultured for 4
h was significantly higher for the animals with the A/A genotype than for those with the G/G genotype. On the other hand, no
significant difference was observed in IL-8 and IL-1β production by PMNs among different TNF-α genotypes. Taken together,
these results suggest that SNP in the TNF-α gene affects immune function and reproductive performance in dairy cows. 相似文献