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MIKI AIDA KAZUYUKI ITOH HIROAKI IKEDA NAOKUNI HARADA YASUO ISHII KENJI USUI 《Weed Biology and Management》2004,4(3):127-135
Paddy herbicides have the potential to cause adverse effects on non-target plants. Susceptibilities of some aquatic ferns ( Azolla japonica Franch. et Savat., Isoetes japonica A. Braun, Marsilea quadrifolia L. and Salvinia natans All.) and duckweeds ( Lemna minor L. and Spirodela polyrhiza Schleid.) to paddy herbicide bensulfuron methyl (BSM) were evaluated with a 20 day exposure experiment using 200 cm2 pots. The BSM concentrations in the surface water of monitoring pots with no plants dissipated exponentially with half lives of 3.5 and 3.9 days at application rates of 15 and 150 g ha−1 , respectively. The BSM concentrations in the surface water 1 day after application in the culture pots were comparable among plant species, and were lower than those in the monitoring pots. Bensulfuron methyl reduced the plant growth in all species. I . japonica showed the lowest intrinsic relative growth rate (RGR) and the lowest susceptibility with an effective dose resulting in 50% growth inhibition (ED50 ) of 21 g ha−1 . Except for I . japonica , the RGR of the duckweeds was similar to the ferns, and ED50 for the duckweeds was higher than the ferns. ED50 for Sa . natans , A . japonica and M . quadrifolia were 1.1, 1.8 and 1.2 g ha−1 , respectively, which were smaller than 1/20 of the recommended field dose (51–75 g ha−1 ) and ranged from 1/2 to 1/6 of ED50 for L . minor and Sp . polyrhiza (6.5 and 3.2 g ha−1 , respectively). These results suggest that BSM application in paddy fields and its runoff in some localities is expected to have adverse effects on the growth of Sa . natans , A . japonica and M . quadrifolia . 相似文献
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Kei ISHII Mito KANATSU-SHINOHARA Takashi SHINOHARA 《The Journal of reproduction and development》2014,60(1):37-46
Spermatogonial stem cells (SSCs) migrate to the niche upon introduction into the
seminiferous tubules of the testis of infertile animals. However, only 5–10% of the
transplanted cells colonize recipient testes. In this study, we analyzed the impact of
cell cycle on spermatogonial transplantation. We used fluorescent ubiquitination-based
cell cycle indicator transgenic mice to examine the influence of cell cycle on SSC
activity of mouse germline stem (GS) cells, a population of cultured spermatogonia
enriched for SSCs. GS cells in the G1 phase are more efficient than those in the S/G2-M
phase in colonizing the seminiferous tubules of adult mice. Cells in the G1 phase not only
showed higher expression levels of GFRA1, a component of the GDNF self-renewal factor
receptor, but also adhered more efficiently to laminin-coated plates. Furthermore, this
cell cycle-dependency was not observed when cells were transplanted into immature pup
recipients, which do not have the blood-testis barrier (BTB) between Sertoli cells,
suggesting that cells in the G1 phase may passage through the BTB more readily than cells
in the S/G2-M phase. Thus cell cycle status is an important factor in regulating SSC
migration to the niche. 相似文献
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Takashige ISHII Kenji KAWASHIMA Haruo ORIBE Hiromi UEDA Toshiya HASUNUMA Kiyoshi AKIYAMA Hirofumi NAKAYAMA Mitsunori KURIHARA Fuminori TERADA Shiro KUSHIBIKI 《Animal Science Journal》2011,82(6):741-746
To decrease the age at first calving in Holsteins, the effects of average daily body weight gain (ADG) and crude protein (CP) level until first insemination on growth performance and milk production were examined. The MM group had a target ADG of 0.75 kg and received a diet with a CP level of 14%. The HM and HH groups had a target ADG of 1 kg; both these groups received a diet with CP levels 14% and 16%, respectively. The ADG in the HM and HH groups was 1.1 kg, whereas in the MM group it was 0.97 kg (P < 0.01). The HM and HH groups showed no differences in withers height at body weight 350 kg. The ages at first calving in MM, HM and HH groups were 23.1, 21.0 and 21.8 months, respectively. The HM and HH groups had lower milk yield at day 305 than the MM group (P < 0.01). These results suggest that growth performance until first insemination should be maintained at an ADG of 0.97 kg or less with a CP level of approximately 14%, to shorten time until first insemination and prevent the decrease of milk yield. 相似文献
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Fumie MAGATA Mitsuo ISHII Eri OIKAWA Hidefumi FURUOKA Kazutaka YAMADA Naoki SASAKI Syo SHIMIZU Hisashi INOKUMA 《Journal of Equine Science》2010,21(2):17-20
A 42-day-old heavy draft horse fell into sudden astasia. Significant swelling and heat
sensation of the left femoral region were observed. Because of a friction sound in the
left hip, we supposed that the hip joint was dislocated or the hip bone was fractured.
Computed Tomography (CT) examination showed that the left hip joint was dislocated and the
left femoral head was disjunct. We carried out a pathological autopsy, and made a
diagnosis of the foal as fracture of the hip bone and femoral head with suppurative
umbilical arteritis. Pathologic changes in the umbilical artery and hind leg were
completely unilateral, suggesting that left umbilical arteritis spread to the blood
circulation, causing arthritis and dislocation of the hip bone. 相似文献
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The possible horizontal transfer of transgenes is of great concern when the transgenic plants are released into the field. To test the possible transfer of nptⅡ of transgenic trees into soil bacteria, we have used a stool DNA preparation kit to isolate the DNA from the soils in the rhizospheres of two non- and eight transgenic Eucalyptus camaldulensis trees. All the samples have provided the corresponding PCR products in the amplification with bacterial 16S RNA specific sequences, which indicates that the quality of the isolated DNA is adequate for amplification. The nptⅡ specific band has been amplified in three soil samples from the transgenic trees and even treated with filtration before the DNA isolation. This indicates that nptlI DNA exists in the soil, although it is still unclear whether the DNA was in the soil particles, in the soil bacteria or in the Agrobaeterium contamination which was used for the E.camaldulensis transformation. Two approaches on isolation of bacterial DNA have been suggested for testing the possibility of this event in the future. 相似文献