Root growth and N‐uptake activity of oilseed rape (Brassica napus L.) cultivars differing in nitrogen efficiency

Nitrate‐N uptake from soil depends on root growth and uptake activity. However, under field conditions N‐uptake activity is difficult to estimate from soil‐N depletion due to different loss pathways. We modified the current mesh‐bag method to estimate nitrate‐N‐uptake activity and root growth of two oilseed‐rape cultivars differing in N‐uptake efficiency. N‐efficient cultivar (cv.) ‘Apex' and N‐inefficient cv. ‘Capitol' were grown in a field experiment on a silty clayey gleyic fluvisol near Göttingen, northern Germany, and fertilized with 0 (N₀) and 227 (N₂₂₇) kg N ha^–1. In February 2002, PVC tubes with a diameter of 50 mm were installed between plant rows at 0–0.3 and 0–0.6 m soil depth with an angle of 45°. At the beginning of shooting, beginning of flowering, and at seed filling, the PVC tubes were substituted by PVC tubes (compartments) of the same diameter, but with an open window at the upper side either at a soil depth of 0–0.3 or 0.3–0.6 m allowing roots to grow into the tubes. Anion‐exchange resin at the bottom of the compartment allowed estimation of nitrate leaching. The compartments were then filled with root‐free soil which was amended with or without 90 mg N (kg soil)^–1. The newly developed roots and nitrate‐N depletion were estimated in the compartments after the installing period (21 d at shooting stage and 16 d both at flowering and grain‐filling stages). Nitrate‐N depletion was estimated from the difference between NO ‐N contents of compartments containing roots and control compartments (windows closed with a membrane) containing no roots. The amount of nitrate leached from the compartments was quantified from the resin and has been taken into consideration in the calculation of the N depletion. The amount of N depleted from the compartments significantly correlated with root‐length density. Suboptimal N application to the crop reduced total biomass and seed‐yield formation substantially (24% and 38% for ‘Apex’ and ‘Capitol’, respectively). At the shooting stage, there were no differences in root production and N depletion from the compartments by the two cultivars between N₀ and N₂₂₇. But at flowering and seed‐filling stages, higher root production and accordingly higher N depletion was observed at N₀ compared to N₂₂₇. Towards later growth stages, the newly developed roots were characterized by a reduction of root diameter and a shift towards the deeper soil layer (0.3–0.6m). At low but not at high N supply, the N‐efficient cv. ‘Apex’ exhibited higher root growth and accordingly depleted nitrate‐N more effectively than the N‐inefficient cv. ‘Capitol’, especially during the reproductive growth phase. The calculated nitrate‐N‐uptake rate per unit root length was maximal at flowering (for the low N supply) but showed no difference between the two cultivars. This indicated that the higher N‐uptake efficiency of cv. ‘Apex’ was due to higher root growth rather than higher uptake per unit of root length.     

Root‐growth characteristics contributing to genotypic variation in nitrogen efficiency of oilseed rape

A 3‐year field experiment was carried out to determine the significance of root‐growth characteristics contributing to N‐uptake efficiency of two oilseed rape (Brassica napus L.) cultivars differing in N efficiency. Two N treatments were applied, and the core and minirhizotron techniques were used to study root‐length density and number of living roots, respectively. Fertilizer‐N supply increased shoot dry matter, grain yield, total N uptake, and total soil N_min contents particularly in the top soil. Although significant differences occurred in all parameters between years, the interactions between years and cultivars were mostly not significant. Compared to cv. Capitol, the N‐efficient cv. Apex was characterized by a higher grain yield at N0 and a higher N uptake during reproductive growth. This genotype also had a higher root‐length density and more living fine roots particularly in the topsoil layer. Root growth of this genotype was especially high from beginning of shooting to beginning of flowering, while shoot growth and N uptake during vegetative growth were comparatively low. Our results suggest that N‐efficient cultivars can be characterized by a high investment in root growth during the vegetative stage with a comparatively slow shoot growth and N‐uptake rate until beginning of flowering, which, however, continues during reproductive growth. High root production only during reproductive growth seems to be less effective to achieve high N efficiency, because this may lead to a shortage of assimilates for seed filling. High root‐length density at vegetative stages may thus be advantageous for N uptake and reproductive growth and could be a useful morphological character for the selection and breeding of N‐efficient cultivars.     

Simrank: Rapid and sensitive general-purpose k-mer search tool

Background  

Terabyte-scale collections of string-encoded data are expected from consortia efforts such as the Human Microbiome Project . Intra- and inter-project data similarity searches are enabled by rapid k-mer matching strategies. Software applications for sequence database partitioning, guide tree estimation, molecular classification and alignment acceleration have benefited from embedded k-mer searches as sub-routines. However, a rapid, general-purpose, open-source, flexible, stand-alone k-mer tool has not been available.     

The Efficacy of Grafting on Alkali Stressed Watermelon Cultivars Under Hydroponic Conditions

The goal of the present study was to determine whether grafting of watermelon on gourd rootstocks could improve alkalinity tolerance and to investigate the physiological and morphological response mechanisms of the grafted plants under different pH levels. The experiment was carried out in a climate chamber to investigate growth, leaf chlorophyll content (SPAD), leaf area, stem length, shoot and root dry weight, root length, electrolyte leakage, leaf mineral composition, total chlorophyll and carotenoid contents. Two watermelon cultivars (Crimson tide, CT, and Crisby) were grafted onto three commercial Cucurbita maxima?×?C. moschata hybrid rootstocks under climate-chamber conditions (Strong tosa, ST, Ercole and Nun 9075). The grafted seedlings were transplanted onto 8?L continuously aerated pots containing nutrient solution with two different pH levels (8.5 and 6.5) and replicated three times. The results showed that in both grafted and non-grafted plants, there was a substantial reduction in shoot and root biomass production at high pH levels. At high pH level, the significantly highest leaf area, stem length, SPAD, concentration of P, Ca, S and Mn in leaf tissues were recorded in graft combination ‘Crisby/Ercole’, whereas the significantly highest concentration of Fe in leaf tissues, shoot dry weight were recorded in graft combination ‘Crisby/Nun 9075’. Moreover, at high pH, the significantly highest concentration of Mg and Cu in shoot under high pH levels was significantly found in graft combination of ‘CT/ST’. These results suggest that the use of interspecific Cucurbita maxima?×?C. moschata hybrid rootstocks can improve crop performance in watermelon plants under alkaline conditions.

     

Comparison of satellite images with different spatial resolutions to estimate stand structural diversity in urban forests

The structural diversity in urban forests is highly important to protect biodiversity. In particular, fruit trees and bush species, cavity-bearing trees and coarse, woody debris provide habitats for animals to feed, nest and hide.Improper silvicultural practices, intensive recreational use and illegal harvesting lead to a decline in the structural diversity in forests within larger metropolitan cities. It is important to monitor the structural diversity at definite time intervals using effective technologies with a view to instituting the necessary conservation measures. The use of satellite images seems to be appropriate to this end. Here we aimed to identify the associations between the textural features derived from the satellite images with different spatial resolutions and the structural diversity indices in urban forest stands(Shannon–Wiener index, complexity index, dominance index and density of wildlife trees).Rapid Eye images with a spatial resolution of 5 m 9 5 m,ASTER images with a spatial resolution of 15 m 9 15 m and Landsat-8 ETM satellite images with a spatial resolution of 30 m 9 30 m were used in this study. The firstorder(standard deviation of gray levels) and second order(GLCM entropy, GLCM contrast and GLCM correlation)textural features were calculated from the satellite images.When associations between textural features in the images and the structural diversity indices were assessed using the Pearson correlation coefficient, very high associations were found between the image textural features and the diversity indices. The highest association was found between the standard deviation of gray levels(SDGLRAP) derived from RVIRAPof Rapid Eye image and the Shannon–Wiener index(Hh) calculated on the basis of tree height(R2= 0.64). The findings revealed that Rapid Eye satellite images with a spatial resolution of 5 m 9 5 m are most suitable for estimating the structural diversity in urban forests.     

Expression of Toll-like receptors and downstream genes in lipopolysaccharide-induced porcine alveolar macrophages

The aim of the present study was to determine the age-related kinetic changes of Toll-like receptors (TLRs) and downstream genes expression, and secretion of cytokine in lipopolysaccharide (LPS) stimulated porcine alveolar macrophages (AM). For this purpose, AMs were isolated from 5-day-old newborn piglets and 120-day-old young pigs. mRNA expression and cytokine measurement was determined by quantitative real-time PCR and ELISA, respectively. First, AMs were incubated for 24 h in the absence or presence of increasing concentrations of LPS. Results showed the up-regulation of TLRs 2, 4, 5 and 9 mRNA from all concentrations of LPS used, as compared to non-stimulated cells, and TLR4 was the highest expression in both ages (P<0.05). Furthermore, quantitative analysis demonstrated increased expression of mRNAs encoding TLRs 2, 4, 5 and 9, LBP, CD14, MD2, MyD88, IRAK4 and TRAF6 in both ages in a time-dependant manner (P<0.05). Overall, LPS inducible mRNA for TLR4, LBP, CD14 and MyD88 had higher expression in newborn piglets compared with those of young pigs (P<0.05). The level of cytokine protein IL6 and TNFα in supernatant fluid significantly varied with time of incubation and age of animals. Their concentration increased immediately at 1 h after LPS stimulation and remained significantly higher up to 48 h in both ages. Production of pro-inflammatory cytokine protein IL6 and TNFα in supernatant was significantly higher in young pigs than those of piglets. This study suggests that differential age-related changes in the expression of TLRs and downstream genes, and pro-inflammatory cytokine could contribute to a different age-related innate immune response during pulmonary infection. Further investigation is warranted to determine the precise effects of LPS on porcine AMs by means of a functional study across a wider age range.     

Determination of Pesticide Residues in Apricot (<Emphasis Type="Italic">Prunus armeniaca</Emphasis> L.) Grown at Good Agricultural Practices (GAPs) by LC-MS/MS and GC-MS

Good Agricultural Practices (GAPs) includes agricultural techniques which environmentally-conscious, is not harmful to human and animal health, target protection of natural resources, provide traceability and food security. With these kinds of production techniques, it is aimed at agricultural production which is socially viable, economically profitable and sustainable. In this study conducted under good agricultural practices, pesticide residues in apricot fruit were investigated. The experiment was set up to have 3 replications and 15 trees in each replicate, according to randomized trial design. From the trees included in the experiment, necessary samples were taken at the harvest time and analyzes were carried out. In this study, pesticide residue levels were determined in fruit extracts with high-precision analytical instruments such as LC-MS/MS and GC-MS. A total of 385 pesticide active substances were analyzed in LC-MS/MS and 101 pesticide active substances in GC-MS in fruit extracts. In this research carried out in 2014 and 2015, samples of both years were not found to be detectable to the tolerance values of Turkish Food Codex (TFC).     

Yield and Quality Response of Young ‘Gala,Galaxy’ Trees under Different Irrigation Regimes

This study was conducted in 2007–2008 to determine the effects of different irrigation regimes on yield, quality and water-yield relationships of young dwarf ‘Gala, Galaxy’ apple trees in Isparta-Turkey. Irrigation water was applied based on a ratio of class A pan evaporation (rates of 0.0 (k_cp0), 0.25 (k_cp1), 0.50 (k_cp2), 0.75 c (k_cp3), 1.00 (k_cp4) and 1.25 (k_cp5)) with 5-day intervals. The effects of irrigation regimes on yield and fruit weight were statistically significant. Seasonal evapotranspiration was measured as 246.5–608.2 mm and 289.3–631.9 mm in 2007 and 2008, respectively. Water use efficiency lead to a yield ranging from 0.0023 to 0.0055 t ha^?1mm^?1 and from 0.0033 to 0.0111 t ha^?1mm^?1 and irrigation water use efficiency varies from 0.0009 to 0.0073 t ha^?1mm^?1 and from 0.0045 to 0.0186 t ha^?1mm^?1 in 2007 and 2008, respectively. The annual average yield response factor, ky, was 1.22. As a result of the research, it was concluded that the irrigation schedule for the 0.75 k_cp3 treatment, applying 0.75 of evaporation from class A pan, could be used in the irrigation of young dwarf apple trees without evident reduction in yield and that high water use efficiencies could be obtained.     

Enhancing Salt Stress Tolerance of Different Pepper (Capsicum annuum L.) Inbred Line Genotypes by Rootstock with Vigorous Root System

Gesunde Pflanzen - In this study, pepper (Capsicum annuum&nbsp;L.) inbred lines were grafted onto different rootstock genotypes and tested under saline conditions. A&nbsp;hydroponic...     

Root-growth Characteristics Contributing to Nitrogen Efficiency of Reciprocally Grafted Potatoes (Solanum tuberosum L.) Under Hyroponic Conditions

Gesunde Pflanzen - To assess whether grafting could improve the nitrogen&nbsp;(N) efficiency of potato cultivars and to determine which physiological and morphological characteristics are...