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Fumio NAMIKI Kanji SHIMIZU Kyoko SATOH Tetsuo HIRABAYASHI Kazufumi NISHI Tsuruo KAYAMURA Takashi TSUGE 《Journal of General Plant Pathology》2000,66(1):12-17
In 1994, Fusarium wilt of melon cultivars which are resistant to races 0 and 2 of Fusarium oxysporum f. sp. melonis was observed in southern area of the Lake Biwa region, Shiga prefecture. In commercial fields, mature plants of cv. Amus
which were grafted onto cv. Enken Daigi 2, and of cv. FR Amus showed yellowing, wilting and finally death before harvesting
of fruits. Diseased plants had vascular and root discolorations, and their stem sections yielded typical colonies of F. oxysporum. When the Shiga strains were tested for their pathogenicity to 12 species of cucurbits, they caused wilts only on melon.
Using race differential cultivars of melon, the Shiga strains were classified as race 1 of F. oxysporum f. sp. melonis, which has not been reported in Japan. To further characterize their pathogenicity, the strains were used to inoculate 46
additional cultivars of melon, oriental melon and oriental pickling melon. All the race 1 strains were pathogenic to the cultivars
tested, and their host range was apparently different from those of strains belonging to other races (races 0, 2 and 1,2y).
DNA fingerprinting with a repetitive DNA sequence, FOLR3, differentiated race 1 strains from strains of races 0 and 2, but
not from race 1,2y strains.
Received 2 July 1999/ Accepted in revised form 30 September 1999 相似文献
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Liyan Wang Liming Bai Daisuke Tokunaga Yusuke Watanabe Toshiaki Hasegawa Jun-ichi Sakai Wanxia Tang Yuhua Bai Katsutoshi Hirose Takao Yamori Akihiro Tomida Takashi Tsuruo Masayoshi Ando 《Journal of Wood Science》2008,54(5):390-401
Twelve basic taxoids and 22 neutral taxoids were isolated from basic and polar neutral fractions of the extracts of needles
and twigs of Taxus cuspidata. Among them, taxine NA-13, 3,11-cyclotaxinine NN-1, taxinine NN-6, 11(15→1)abeo-taxinine NN-1, taxine NA-8, and taxine NA-4 were isolated first from natural sources by us. The cytotoxic activity of isolated
compounds was evaluated against three human cell lines: normal human fibroblast cells (WI-38), malignant tumor cells induced
from WI-38 (VA-13), and human liver tumor cells (HepG2). 7-Epitaxol, 7-epicephalomannine, taxinine NN-6, taxine NA-2, taxuspine
H, and taxagifine were active toward VA-13 cells and 7-epitaxol, 7-epicephalomannine, taxinine NN-1, 9,10-deacetyltaxinine,
and taxagifine were active toward HepG2 cells. The multidrug-resistant (MDR) cancer reversal activity of isolated compounds
was evaluated on the basis of the amount of vincristine (VCR) accumulated in MDR human ovarian cancer 2780 AD. Taxine NA-8,
taxine NA-2, 5-cinnamoyl-10-acetyltaxicin II, and taxinine NN-1 indicated stronger MDR cancer reversal activity than verapamil.
The result of primary screening based on 39 human cancer cell lines suggests that taxinine NN-1 belongs to a new mechanistic
class and is a new anticancer agents. 7-Epicephalomannine was found to be an effective anticancer agent with tubulin as its
molecular target, which is the same as paclitaxel.
Part of this work was presented at the 74th Annual Meeting of the Chemical Society of Japan, Tanabe, March 1998, and the 41st
Symposium on the Chemistry of Terpenes, Essential Oils, and Aromatics, Gifu, December 1998. The results of this work were
also presented as patent documents at PCT Int: Appl WO 2001007040 A1, February 2001; Appl WO 2000-Jp 5036, 27 July 2000. Priority:
JP 1999-214273, 28 July 1999; JP 1999-224652, 6 August 1999; JP 2000-76404, 14 March 2000 相似文献
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