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1.
Within a research project on quality of plants for forestation of agricultural lands, we studied the aetiology of a late seedling rot affecting holm oak (Quercus ilex ssp. ballota) in two forest nurseries in southern Spain. Major disease symptoms were foliage wilting and necrosis of feeder roots. Phytophthora cinnamomi, Phytophthora cryptogea and Phytophthora drechsleri were isolated from necrotic roots of holm oaks. Selected isolates of the three Phytophthora species were pathogenic to Quercus ilex ssp. ballota and Quercus suber seedlings in artificial inoculations. Soil flooding conditions were essential for infection and root rot development. There was no host specificity among the species, the isolates of P. cinnamomi being the most virulent in all inoculated plants. In these inoculations, Q. ilex ssp. ballota plants were more susceptible than those of Q. suber. This work is the first report of P. cinnamomi, P. drechsleri and P. cryptogea affecting Q. ilex ssp. ballota in forest nurseries. 相似文献
2.
The influence of temperature, wetness duration, and planting density on infection of olive fruit by Colletotrichum acutatum and C. simmondsii was examined in laboratory and field experiments. Detached olive fruit of 'Arbequina', 'Hojiblanca', and 'Picual' were inoculated with conidia of several isolates of the pathogen and kept at constant temperatures of 5 to 35°C in humid chambers. Similarly, potted plants and stem cuttings with fruit were inoculated and subjected to wetness periods of 0 to 48 h. Infection occurred at 10 to 25°C, and disease severity was greater and the mean latent period was shorter at 17 to 20°C. Overall, C. acutatum was more virulent than C. simmondsii at temperatures <25°C. When temperature was not a limiting factor, disease severity increased with the wetness period from 0 to 48 h. Disease severity was modeled as a function of temperature and wetness duration; two critical fruit incidence thresholds were defined as 5 and 20%, with wetness durations of 1.0 and 12.2 h at the optimum temperature. In the field, anthracnose epidemics progressed faster in a super-high-density planting (1,904 olive trees/ha) than in the high-density plantings (204 to 816 olive trees/ha) and caused severe epidemics in the super-high-density planting even with the moderately resistant Arbequina. Data in this study will be useful for the development of a forecasting system for olive anthracnose epidemics. 相似文献
3.
Variation of pathotypes and races and their correlations with clonal lineages in Verticillium dahliae
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R. M. Jiménez‐Díaz C. Olivares‐García J. L. Trapero‐Casas M. M. Jiménez‐Gasco J. A. Navas‐Cortés B. B. Landa M. G. Milgroom 《Plant pathology》2017,66(4):651-666
Understanding pathogenic variation in plant pathogen populations is key for the development and use of host resistance for managing verticillium wilt diseases. A highly virulent defoliating (D) pathotype in Verticillium dahliae has previously been shown to occur only in one clonal lineage (lineage 1A). By contrast, no clear association has yet been shown for race 1 with clonal lineages. Race 1 carries the effector gene Ave1 and is avirulent on hosts that carry resistance gene Ve1 or its homologues. The hypothesis tested was that race 1 arose once in a single clonal lineage, which might be expected if V. dahliae acquired Ave1 by horizontal gene transfer from plants, as hypothesized previously. In a diverse sample of 195 V. dahliae isolates from nine clonal lineages, all race 1 isolates were present only in lineage 2A. Conversely, all lineage 2A isolates displayed the race 1 phenotype. Moreover, 900‐bp nucleotide sequences from Ave1 were identical among 27 lineage 2A isolates and identical to sequences from other V. dahliae race 1 isolates in GenBank. The finding of race 1 in a single clonal lineage, with identical Ave1 sequences, is consistent with the hypothesis that race 1 arose once in V. dahliae. Molecular markers and virulence assays also confirmed the well‐established finding that the D pathotype is found only in lineage 1A. Pathogenicity assays indicated that cotton and olive isolates of the D pathotype (lineage 1A) were highly virulent on cotton and olive, but had low virulence on tomato. 相似文献
4.
P. Morello C. M. Díez M. Codes L. Rallo D. Barranco A. Trapero J. Moral 《Plant pathology》2016,65(3):412-421
Verticillium wilt, caused by the fungus Verticillium dahliae, is currently the most important disease affecting olive in the Mediterranean basin. There are no effective treatments for controlling this disease. The use of infected nursery stocks has largely contributed to the spread of the pathogen, and therefore the development of treatments to preventively sanitize the propagation stock is critical in the nursery industry. This study describes novel techniques to achieve this aim. The effects of several temperature–exposure time combinations were evaluated: (i) the survival of pathogen on culture medium (PDA); (ii) the pathogen viability on infected shoots and plants; (iii) the vegetative growth of plants of several cultivars; and (iv) the rooting ability of cuttings. The colonies of the pathogen growing in PDA were killed after 8 h and 60 min of exposure at 40 and 47°C, respectively. Temperatures ≥42°C for at least 2 h were lethal for the pathogen infecting the shoots. Likewise, moist hot air treatments at 42–44°C for 6–12 h eradicated the pathogen, without compromising the viability of the plants. Five olive cultivars were also evaluated and classified according to their thermotolerance as follows: sensitive (Chiquitita), moderately sensitive (Koroneiki, Frantoio and Picual) and heat tolerant (Arbequina). However, the optimized sanitation methods were applicable to all of the cultivars. Finally, heat treatments were applied to unrooted cuttings, which severely affected their rooting ability. Thus, this study developed a hot air treatment to produce V. dahliae‐free olive nursery plants. 相似文献
5.
Characterization of resistance against the olive‐defoliating Verticillium dahliae pathotype in selected clones of wild olive
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D. Jiménez‐Fernández J. L. Trapero‐Casas B. B. Landa J. A. Navas‐Cortés G. Bubici M. Cirulli R. M. Jiménez‐Díaz 《Plant pathology》2016,65(8):1279-1291
Verticillium wilt of olive is best managed by resistant cultivars, but those currently available show incomplete resistance to the defoliating (D) Verticillium dahliae pathotype. Moreover, these cultivars do not satisfy consumers' demand for high yields and oil quality. Highly resistant rootstocks would be of paramount importance for production of agronomically adapted and commercially desirable olive cultivars in D V. dahliae‐infested soils. In this work, resistance to D V. dahliae in wild olive clones Ac‐13, Ac‐18, OutVert and StopVert was assessed by quantifying the fungal DNA along the stem using a highly sensitive real‐time quantitative polymerase chain reaction (qPCR) protocol and a stem colonization index (SCI) based on isolation of V. dahliae following artificial inoculations under conditions highly conducive for verticillium wilt. Ac‐13, Ac‐18, OutVert and StopVert showed a symptomless reaction to D V. dahliae. The mean amount of D V. dahliaeDNA quantified in stems of the four clones ranged from 3.64 to 28.89 pg/100 ng olive DNA, which was 249 to 1537 times lower than that in susceptible Picual olive. The reduction in the quantitative stem colonization of wild olive clones by D V. dahliae was also indicated by a sharp decrease in the SCI. Overall, there was a pattern of decreasing SCI in acropetal progression along the plant axis, as well as correlation between positive reisolation and quantification of pathogen DNA. The results of this research show that wild olive clones Ac‐13, Ac‐18, OutVert and StopVert have a valuable potential as rootstocks for the management of verticillium wilt in olive. 相似文献
6.
M.E.Sánchez Hernández A. Ruiz Dávila A. Pérez de Algaba M.A. Blanco López A. Trapero Casas 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(4):347-357
New plantations of olive tree in southern Spain are being severely affected by wilt or dieback and death, which has been locally called Drying Syndrome. To determine the etiology of this problem, a study was carried out in samples of affected young trees collected during a seven year period (1989–1995), and in two field surveys in 1994–95 and 1996. Besides some insect damage and agronomic problems, the Drying Syndrome was associated with Verticillium wilt, winter frost and root rot fungi. Although Drying Syndrome can be distinguished from Verticillium wilt, the latter was included in this study, since, frequently, Verticillium wilt symptoms were unspecific and Verticillium dahliae could not be always isolated in the diagnostic work that preceded this study. Early winter frost caused a vascular necrosis and wilt of the young olive trees. This unusual and severe damage was related with the lack of frost hardiness due to warm temperatures during the previous autumn. Root rot fungi were very frequent in the samples of diseased olive trees of field or nursery origin, and they were the main cause of Drying Syndrome in the second field survey, when a heavy rainfall level occurred during winter. Pathogenicity tests showed that five fungal species (Cylindrocarpon destructans, Phytophthora megasperma, P. palmivora, Pythium irregulare and Sclerotium rolfsii) were pathogenic to olive trees and reproduced symptoms of Drying syndrome in rooted cuttings of cultivar Picual. Other fungal species associated with root rot of olive trees in the field or in the nurseries, including Fusarium acuminatum, F. eumartii, F. oxysporum, F. solani, Macrophomina phaseolina and Rhizoctonia solani, were weakly or not pathogenic. Pathogenicity of P. megasperma, P. palmivora and P. irregulare depended on soil water content, since isolates tested caused extensive root rot and sudden plant death only when the soil was continuously waterlogged. The high frequency of P. megasperma in waterlogged field soils and its pathogenicity dependence on soil water content suggest that this pathogen may play an important role in the well known sensitivity of young olive trees to root asphyxiation. 相似文献
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8.
Pedro Valverde Carlos Trapero Octavio Arquero Nicolás Serrano Diego Barranco Concepción Muñoz Díez Francisco J. López-Escudero 《Plant pathology》2021,70(1):144-153
Verticillium wilt of olive (VWO) is probably the most devastating fungal disease for olive trees worldwide, and currently the main cultivars are susceptible or moderately susceptible to this disease. The evaluation of resistant cultivars as rootstocks to control the disease has scarcely been explored, and mainly in short-term studies under controlled conditions, which usually do not correspond with field evaluations. The main objective of this study was to assess the responses to VWO of different scion × rootstock combinations of the olive cultivars Picual, Arbequina, Changlot Real, and Frantoio in a long-term field experiment with a soil highly infested with the defoliating pathotype of Verticillium dahliae. The results showed that grafting the susceptible cultivar Picual onto resistant rootstocks delayed the onset of the disease symptoms; however, after 4 years, it was observed that all combinations that contain Picual (a) were extensively colonized by V. dahliae; (b) developed severe symptoms of the disease; and (c) had plant mortality similar to Picual growing on its own roots. This result highlights the importance of long-term field experiments to evaluate VWO and shows that grafting susceptible olive cultivars onto resistant ones does not provide a durable control of VWO under high inoculum potential, as V. dahliae is able to progress through the resistant rootstock and then extensively colonize and kill the susceptible scion. However, the high inoculum potential observed in this study does not allow us to consider the evaluated resistant cultivars as completely ineffective under lower inoculum densities. 相似文献
9.
M. E. Snchez N. Luchi J. J. Jimnez P.
De Vita J. E. Snchez A. Trapero P. Capretti 《Forest Pathology》2007,37(5):348-356
Abies pinsapo, a fir endemic to a small area in southernmost Spain, is frequently attacked in natural stands by Heterobasidion root rot. To identify the Heterobasidion species causing the disease, pure cultures of the fungus were isolated from symptomatic trees in five localities in Sierra de las Nieves Natural Park and identified by pairing tests. In addition, genets of the fungus were identified in two pure stands of A. pinsapo. All the Heterobasidion specimens collected belonged to the species Heterobasidion abietinum. The largest genet found was 57 m long, and had colonized 10 trees. The large size of the main genets implied that H. abietinum had spread via root contacts from old infections generated before the establishment of the Sierra de las Nieves National Park in 1989. Exceptionally dry summers in the last 20 years may have weakened A. pinsapo and favoured spread of the disease. 相似文献
10.
Romero Joaqun Santa-Brbara Ana Esperanza Moral Juan Agust-Brisach Carlos Roca Luis F. Trapero Antonio 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(3):545-556
European Journal of Plant Pathology - Anthracnose, caused by Colletotrichum spp., is the main olive fruit disease. Colletotrichum can severely infect olive fruit with a negative impact on the oil... 相似文献