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1.
T. KATAN 《EPPO Bulletin》1985,15(3):371-377
Field isolates of Botrytis cinerea with moderate levels of resistance to dicarboximide fungicides (ED50 1.0–4.9 μg ml?1) and to dicloran were obtained from glasshouses where vinclozolin and iprodione failed to control grey mould. From sensitive and moderatcly-resistant cultures, laboratory isolates were selected on dicarboximide-amended medium, which were highly resistant to these fungicides (ED50 125->3000 μg ml?1). Conidia of all the resistant isolates germinated well on media amended with 100 μg ml?1 of the dicarboximides vinclozolin, iprodione, procymidone and myclozolin and with 5 μg ml?1 of metomeclan. However, the spores of the moderately resistant isolates did not germinate on 100 μg ml?1 metomeclan while the spores of the highly resistant isolates germinated well. Using media with 100 μg ml?1 of metomeclan to distinguish between the two phenotypes, no highly resistant strain was detected among 312 resistant samples from five cucumber glasshouses with a high frequency of moderately resistant strains. From air-borne inoculum of five glasshouses with 100% resistant populations, 1604 colonies were recovered on vinclozolin-amended (100 μg ml?1) medium and none on metomeclan-amended (100 μg ml?1) medium. It is concluded that strains of B. cinerea highly resistant to dicarboximides are absent from field populations. 相似文献
2.
Summary. A comparison was made between diphenamid and napropamide with regard to phytotoxicity to pepper and weeds under glasshouse and field conditions. Diphenamid was considerably less phytotoxic than napropamide in inhibiting root elongation and shoot growth of pepper seedlings. Plant growth was reduced when the roots were exposed to either one of the herbicides, but growth of the shoot through treated soil was not adversely affected. Since diphenamid was found to be more leachable into soil, it might become more available to the roots and damage the crop plants. Graminaceous weeds were very sensitive to both herbicides, whereas several dicotyledonous weeds were more susceptible to napropamide. Selectivity of both herbicides at a late pre-emergence application to direct-seeded pepper was found satisfactory in two field experiments on different soil types.
Action sélective de la diphénamide et de la napropamide sur le piment et les mauvaises herbes 相似文献
Action sélective de la diphénamide et de la napropamide sur le piment et les mauvaises herbes 相似文献
3.
Auxotrophic mutants were used to determine vegetative relatedness among isolates of Fusarium oxysporum f.sp. dianthi (F.o.d.) , the vascular wilt pathogen of carnation. At the first stage, different nitrate-non-utilizing (nit) mutants were produced from 11 isolates of F.o.d. collected in Israel. Complementation (heterokaryon) tests showed that all the isolates belonged to a single vegetative compatibility group (VCG), and two mutants were chosen as its testers. Additional isolates of Fusarium from carnation, collected during 1986-88, were analysed for pathogenicity and vegetative compatibility with the testers. A total of 170 Fusarium isolates, obtained from 42 cultivars at 40 sites, were tested. All the nit mutants of all the 132 pathogenic isolates formed heterokaryons with the testers, indicating that they belonged to the same VCG. None of the 38 non-pathogenic isolates was vegetatively compatible with the testers. The nit mutants retained pathogenicity to carnation. The F.o.d. testers were not compatible with testers of five other formae speciales of F. oxysporum. Thus, F.o.d. appears to constitute a distinct genetic population within the F. oxysporum complex. 相似文献
4.
Studies were made of plant growth retardation and effects on resistance of melon to fusarium wilt by soil application of paclobutrazol, other ergosterol biosynthesis inhibitors, the fungicide benomyl and the herbicide dinitramine. Paclobutrazol and ancymidol delayed the onset of wilting and were the most effective in reducing wilt incidence. A relationship between effectiveness in retarding seedling elongation and increasing resistance to fusarium wilt was observed. No chemical had a significant effect on pathogen population level in the plant, as determined by stem colonization and direct assessment tests in seedlings grown in soil treated with the chemicals. Excluding benomyl, and to a lesser extent dinitramine, leaf and stem extracts had no inhibitory effect on conidial germination. Gibberellins GA4+7 , when applied to seedlings inoculated after germination in paclobutrazol-treated soil, nullified growth retardation and increased disease incidence. We suggest that disease incidence reduction by paclobutrazol is due to an effect on plant metabolic processes and not to direct fungitoxicity of the compound. 相似文献
5.
Several film-forming polymers reduced the amount of grey mould on various crops in a dew chamber and in a plastic house under natural conditions. The polymers Wilt Pruf, Biofilm and Colfix reduced germination of conidia and germ tube length of Botrytis cinerea . The most effective inhibition of linear growth of the pathogen on potato dextrose agar was obtained by Safe Pack and Biofilm. All polymers significantly reduced grey mould on detached leaves of Capsicum, Phaseolus , tomato, cucumber, rose and pelargonium. Grey mould on rose flowers was not controlled, apparently due to latent infection. The substances Biofilm and Vapor Gard were applied either alone or with chlorothalonil fungicide on cucumber plants in a commercial greenhouse. The polymers had no harmful effect to the host. Disease on senescing female fruits of cucumber was reduced by 46–67% with no additive effect to the mixture with fungicides. Stem infection also was reduced. 相似文献
6.
Negative cross resistance between benzimidazole and N-phenylcarbamate fungicides and control of Botrytis cinerea on grapes 总被引:4,自引:1,他引:4
Isolates of Botrytis cinerea resistant to benzimidazoles (BenR ), dicarboximides (DicR ), or both (BenR DicR ) were found on table grapes in vineyards in Israel. In vineyards treated for one or two seasons with benomyl and dicarboximides, 41% of the isolates were benomyl-resistant, 18% were dicarboximide-resistant and 21 % were resistant to both fungicides. The frequency of resistant strains was very low in non-treated vineyards. Negatively correlated cross resistance (NCCR) was manifested by 46 BenR isolates which were sensitive to isopropyl N -(3,4-diethoxyphenyl) carbamate (NPC) and 73 benomyl-sensitive (Bens ) isolates which were insensitive to NPC; NCCR was not influenced by sensitivity to dicarboximides. A mixture of Bens DicR plus BenR Dics isolates, inoculated on rose petals, was inhibited by mixtures of benzimidazoles plus NPC but not by benomyl, NPC or iprodione alone. Grey mould, incited on maturing grape berries by a vineyard population composed of Bens and BenR strains, was not controlled by benomyl, while the mixture of NPC plus carbendazim was effective. 相似文献
7.
Resistance to diethofencarb (NPC) in benomyl-resistant field isolates of Botrytis cinerea 总被引:4,自引:3,他引:4
Based on the 'negative cross-resistance' phenomenon between N -phenylcarbamates and benzimidazole fungicides, the fungicidal mixture of carbendazim + diethofencarb (MBC + NPC) was introduced, to control Botrytis cinerea phenotypes which are either sensitive to benzimidazoles and resistant to NPC (wild-type: BenS NPCR ). or resistant to benzimidazoles and sensitive to NPC(BenR NPCS ). At one out of four sites where the MBC + NPC mixture was used in commercial cucumber greenhouses, grey mould control failed and a new phenotype of B. cinerea was found. The new phenotype was resistant to benzimidazoles, as was 100% of the population screened in the four sites, but retained resistance to NPC. Accordingly, the new phenotype was designated BenR NPCR . It was pathogenic on cucumber seedlings. 相似文献
8.
Failures to control grey mould by dicarboximides on winter-grown cucumbers in glasshouses in Israel were attributed to the development of resistance to these fungicides in populations of Botrytis cinerea. A survey of 18 glasshouses in the winter of 1983-84 revealed that resistance is widespread where dicarboximides have been used extensively. During the summer, resistant B. cinerea populations often shift back to sensitivity; however, following few dicarboximide sprays in the subsequent winter, resistant strains reappear, increase rapidly and become dominant in the pathogen population. In two out of three glasshouse experiments, combination of chlorothalonil and dicarboximides in spray programmes slowed down the increase of resistant strains, whereas in a third experiment such a programme had no effect. The usefulness of chlorothalonil in fungicide-alternation programme to suppress dicarboximide resistance is dicussed in relation to the fitness properties of established versus newly-appearing resistant mutants. 相似文献
9.
Multiple fungicide resistance to benzimidazoles, dicarboximides and diethofencarb in field isolates of Botrytis cinerea in Israel 总被引:5,自引:1,他引:4
During the period January-March 1989, 15 greenhouses at 12 sites in Israel were surveyed for the presence of fungicide-resistant strains of Botrytis cinerea , using a fungicide-amended Botrytis -selective medium. Resistance to benzimidazoles (BenR ) and to dicarboximides (DicR ) was frequent in most sites. Resistance to carbendazim + diethofencarb (BenR NPCR ) was found in all eight sites in which a mixture of these fungicides had been used against grey mould, but not in other sites. A new phenotype of multiple fungicide resistance was found among these isolates. The new phenotype, designated BenR DicR NPCR , combines the three previously described characteristics of resistance to benzimidazole, dicarboximide and N -phenylcarbamate fungicides. It was found only in cucumber greenhouses that had been sprayed with the fungicide mixture carbendazim + diethofencarb against grey mould. Isolates of this phenotype were pathogenic in artificial inoculation of cucumber cotyledons treated with carbendazim, iprodione or carbendazim + diethofencarb. 相似文献
10.
Fusarium wilt and crown rot of sweet basil, caused by Fusarium oxysporum f.sp. basilici (F.o.ba.), is widespread in Israel. Affected plants show a variety of symptoms, including vascular wilt as well as crown rot, and masses of macroconidia on stem surfaces. We used vegetative compatibility to determine whether F.o.ba. isolates associated with various symptoms and sources are genetically related. All 119 isolates previously described as F.o.ba., and 42 additional F. oxysporum isolates which had not been tested for pathogenicity, belonged to a single vegetative compatibility group (VCG). The various symptoms are therefore induced by a single pathogenic form which appears to be a specific clone of F. oxysporum. The isolates of F.o.ba. from Israel were vegetatively compatible with eight isolates of F.o.ba. from Italy and the USA, but not with nonpathogenic isolates of F. oxysporum from basil, or with F.o. lycopersici or F.o. radicis-lycopersici from tomato. We conclude that the population of F.o.ba. in Israel belongs to the common VCG of this pathogen described in the USA, and which includes American and Italian isolates. 相似文献