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Katakura K Mimori T Furuya M Uezato H Nonaka S Okamoto M Gomez L EA Hashiguchi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):649-653
PCR amplification and nucleotide sequencing of the mini-exon gene revealed that four strains isolated from a sloth (Choloepus hoffmanni), a squirrel (Sciurus granatensis) and two sandflies (Lutzomyia hartmanni) in Ecuador were indistinguishable from Endotrypanum monterogeii. Another strain isolated from Lu. hartmanni showed the high sequence similarity to E. schaudinni. Since three of these strains have been previously identified as Leishmania (Viannia) equatorensis, the results demonstrate that L. (V.) equatorensis is genetically closely related to the genus Endotrypanum. The present study also indicates that Endotrypanum species are distributed in arboreal animals and sandflies in Ecuador, and that mini-exon gene amplification is useful for epidemiological studies of Leishmania and Endotrypanum in the New World. 相似文献
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Kazuo Yagi Kazuhiko Akimoto Norihiko Mimori Toshiro Miyake Masaki Kudo Kazutaka Arai Shigeru Ishii 《Pest management science》2000,56(1):65-73
A series of novel 3‐(2,4,6‐trisubstituted phenyl)uracil derivatives has been synthesised and assayed for insecticidal/acaricidal activity. The assay indicated certain requirements for optimal insecticidal activity, which can be summarised as follows: (a) the substituents on the phenyl ring should possess hydrophobicity and electron‐withdrawing properties, and the sum of their volumes determines the level of activity; (b) the substituent at the 6‐position on the uracil ring should also possess electron‐withdrawing properties and hydrophobicity, together with the correct volume; (c) the 1‐position on the uracil ring should be unsubstituted for activity against Nephotettix cincticeps and Epilachna vigintioctopunctata, but substituents with length C3 to C4 may be optimal for activity against Tetranychus urticae; (d) certain substituents at the 5‐position of the uracil ring give activity against E vigintioctopunctata and T urticae, but not against N cincticeps; (e) a thiocarbonyl group at the 2‐position of the uracil ring is less effective than a carbonyl group. Of the compounds assayed, 3‐(2,6‐dichloro‐4‐trifluoromethylphenyl)‐6‐trifluoromethyluracil showed high activity against all the species assayed. © 2000 Society of Chemical Industry 相似文献
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Nario Kusano Keita Hirashima Minoru Kuwahara Kenji Narahara Tadashi Imamura Tomohiro Mimori Ken Nakahira Kuniaki Torii 《Journal of General Plant Pathology》2007,73(1):66-71
A simple and rapid immunochromatographic assay (ICA) to detect Satsuma dwarf virus (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Of six homogenization buffers tested,
0.1 M citrate buffer (pH 7.0) gave the best results for the ICA. In the ICA, addition of 0.1% thioglycolic acid in the homogenization
buffers that have been widely used in enzyme-linked immunosorbent assays (ELISA) was deleterious to the reaction because of
undesirable coagulation of the colloidal gold. ICA using the anti-SDV monoclonal antibodies was 8 times and 16 times more
sensitive than double antibody sandwich-ELISA and ICA using the anti-SDV polyclonal antibody, respectively. The analysis is
complete in only 15 min. Furthermore, ICA using the anti-SDV monoclonal antibodies could also detect SDV-related viruses. 相似文献
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Synthesis and insecticidal activity of novel 1,3,4-oxadiazolin-5-one and pyrazolin-5-one derivatives
Kazuo Yagi Akira Numata Norihiko Mimori Toshiro Miyake Kazutaka Arai Shigeru Ishii 《Pest management science》1999,55(2):161-165
A series of novel 2-(2,4,6-trisubstituted phenyl)-1,3,4-oxadiazolin-5-one derivatives and 3-(2,4,6-trichlorophenyl)pyrazolin-5-one derivatives were synthesized and evaluated for insecticidal activity. It was found that a moderately bulky alkyl group, such as a tert-butyl group, on the heterocyclic ring, and a trifluoromethyl group on the benzene ring were optimal substituents on the molecule. The oxygen atom in the oxadiazoline ring was essential for insecticidal activity. Of the compounds assayed, 4-tert-butyl-2-(2,6-dichloro-4-trifluoromethylphenyl)-1,3,4-oxadiazolin-5-one gave the highest activity against Nephtotettix cincticeps, with an LC50 value of 0.51 mg litre−1. © 1999 Society of Chemical Industry 相似文献
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By the experimental study about the effect of a circulate loading on the deformation and the Young's model of Sanjome andesite at the distinct strain rate in a low stress state, the viscoelasticity of Sanjome andesite in a low stress state is researched by means of the effect of the circulate loading on the deformation of rocks. It is found that the natural material such as rocks has obvious viscoelasticity, and the mechanical property of rocks has a direct bearing on the size, direction and loading rate, but also on the way of loading and the organization attribute of rocks. 相似文献
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