排序方式: 共有2条查询结果,搜索用时 0 毫秒
1
1.
Fumiko TAGUCHI Rui TANAKA Sayuri KINOSHITA Yuki ICHINOSE Yoshiyuki IMURA Salamah ANDI Kazuhiro TOYODA Tomonori SHIRAISHI Tetsuji YAMADA 《Journal of General Plant Pathology》2001,67(2):116-123
To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P. s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and
potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using
recombinant harpin indicated harpinpsta was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P. s. pathovars in hrp-inducing medium, the truncated harpinpsta was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant
of the host-parasite specificity in P. s. pv. tabaci.
Received 10 August 2000/ Accepted in revised form 21 December 2000 相似文献
2.
K. Tumirah S. Salamah A. Rozita U. Salmiah M. A. M. Nasir 《Wood Science and Technology》2012,46(6):1021-1031
Ultraviolet–visible Spectrophotometer method for the determination of 2-thiocyanomethylthiobenzothiazole (TCMTB) in treated wood and wood preservative was developed. The determination was done after conversion of TCMTB to 2-mercaptobenzothiazole (2-MBT). This conversion was obtained by reaction with 0.1?M cystein alkaline condition (pH 10). The extraction of 2-MBT was carried out with ethyl acetate containing 0.2% of ?-mercaptoethanol after acidification with 2?M phosphoric acid (pH 2.5). The sample absorbance was measured at 324?nm wavelength with a calibration curve range from 10 to 90?ppm of TCMTB. The Regression value, R 2, achieved was 0.9998, while the precision, RSD, was <10% (n?=?6). The recovery was in the range of 90.7–103.2% (n?=?6). The result obtained with this colorimetric procedure is comparable to the HPLC–PDA method. 相似文献
1