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1.
Christophe Tastet Florence Val Michel Lesage Lionel Renault Laurent Marché Michel Bossis Didier Mugniéry 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(8):821-832
Two major proteins, Mcf-A67 and Mcf-B66, were identified by mini two-dimensional polyacrylamide gel electrophoresis in order to distinguish the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from eight other species. These quarantine proteinic markers have been microsequenced after enzymatic digestion. The internal amino acid sequences exhibit similarities to members of a family of low molecular weight intracellular lipid-binding proteins. Moreover, to explore a simple, rapid, and inexpensive way to identify the two quarantine nematodes, dot blot hybridizations were performed using an antiserum (A67) produced from the longest amino-acid sequence of the protein Mcf-A67. Although several proteins stained on the M. chitwoodi and M. fallax western blot membranes, the two nematodes were easily distinguished from other root-knot nematodes, on dot blot assays with soluble proteins extracted from a single female. Because of its specificity and sensitivity, the use of the A67 antiserum to improve the diagnosis of the two European quarantine root-knot nematodes is discussed. 相似文献
2.
Atlas R Campbell P Cozzarelli NR Curfman G Enquist L Fink G Flanagin A Fletcher J George E Hammes G Heyman D Inglesby T Kaplan S Kennedy D Krug J Levinson R Marcus E Metzger H Morse SS O'Brien A Onderdonk A Poste G Renault B Rich R Rosengard A Salzburg S Scanlan M Shenk T Tabor H Varmus H Wimmer E Yamamoto K;Journal Editors Authors Group 《Science (New York, N.Y.)》2003,299(5610):1149
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G.G.A. Godaliyadda D. Renault H.M. Hemakumara I.W. Makin 《Irrigation and Drainage Systems》1999,13(1):33-55
Improvements to normal operational procedures are investigated for the main types of irrigation system identified in Sri Lanka. Scheduled and unscheduled changes of flow regime the systems are likely to experience and the opportunities for improved operations related to the physical characteristics of each type are analyzed.Performance of several alternative manual operational procedures are evaluated and compared using a hydraulic model (Simulation of Irrigation Canal, SIC) for three different irrigation subsystem types (Single Bank canal; Double Bank canal; Canals with intermediate Storage). Results of these simulations show that current practice, based on a fixed frequency of operation of 12 hrs and a target water depth set to the spill level of the cross-regulator, is not far from the optimal for systems without on-line storage. A slight improvement can be expected if the frequency of operation is increased to 6 hrs. For systems with on-line storage it is recommended to split operational procedures into two parts, one related to the canal and one to the storage. Among different options investigated, discharge control at the downstream regulator of the storage appears to be the most reliable as it performs well whatever operation procedures are selected for the canal.Irrigation systems in Sri Lanka are subject to frequent positive flow changes (Supply fluctuations, Runoff, Return-flow changes). As far as water management is concerned, improvements to be expected from normal operation procedures appear to be somewhat limited because management of flow changes is not an objective. For instance little effort is made to harvest runoff under the current practices. One conclusion of the study is that new strategies for the harvest, storage and release of positive flow changes should be tested and, where appropriate, implemented to improve the overall efficiency of operation in irrigation systems in Sri Lanka. 相似文献
5.
Selection of nematodes by resistant plants has implications for local adaptation and cross‐virulence
S. Fournet M. C. Kerlan L. Renault J. P. Dantec C. Rouaux J. Montarry 《Plant pathology》2013,62(1):184-193
The variability of resistance durability in different potato genotypes harbouring the same resistance QTL but differing by their genetic background was explored. The indirect consequences of the resistance adaptation in terms of local (i.e. genotype‐specific) adaptation and cross‐virulence was also investigated. Following the virulence of the potato cyst nematode Globodera pallida in a long‐term experimental evolution protocol, the results showed that nematode populations were able to adapt to the resistance of four potato genotypes carrying the QTL GpaV from Solanum vernei, and that the plant genetic background has an impact upon the durability of resistance. The pattern of local adaptation observed here indicates that divergent selection has occurred during the experimental evolution performed from the same initial nematode population, and revealed a trade‐off between the adaptation to a resistant potato genotype and the adaptation to another resistant genotype differing in its genetic background. In terms of cross‐virulence between potato genotypes derived from different resistance sources (S. sparsipilum and S. spegazzinii), this study shows that the adaptation to resistance QTL GpaVvrn does not necessarily allow the adaptation to collinear GpaV loci. The results presented here could be useful for predicting evolution of nematode populations in natural agro‐ecosystems and identifying durable strategies for resistance deployment. 相似文献
6.
Ostreid herpesvirus 1 detection and relationship with Crassostrea gigas spat mortality in France between 1998 and 2006 总被引:1,自引:0,他引:1
Garcia C Thébault A Dégremont L Arzul I Miossec L Robert M Chollet B François C Joly JP Ferrand S Kerdudou N Renault T 《Veterinary research》2011,42(1):73
ABSTRACT: Since its molecular characterisation, Ostreid herpesvirus 1 (OsHV-1) has been regularly detected in Crassostrea gigas in France. Although its pathogenicity was demonstrated on larval stages, its involvement during mortality outbreaks at the juvenile stage was highly suspected but not evidenced. To investigate mortality outbreaks, the French National Network for Surveillance and Monitoring of Mollusc Health (REPAMO) carried out two surveys in juvenile C. gigas. The first survey lasted from 1998 to 2006 and was an epidemiological inquiry occurring when oyster farmers reported mortality outbreaks. The second survey, a longitudinal one, was set up in 1998 to complete the network observations on OsHV-1. Data analysis showed a specific pattern of mortality outbreaks associated with OsHV-1 detection. Ostreid herpesvirus 1 detection mainly appeared during the summer, suggesting the influence of the seawater temperature on its occurrence. It mostly presented a patchy distribution in the field in contrast to the nursery. Significant relationship between OsHV-1 detection and spat mortality was found, preferentially in sheltered and closed environments. The longitudinal survey confirmed most of the network observations. Although subsequent works particularly epidemiological surveys would be useful to confirm the causal link between the detection of OsHV-1 and the mortality outbreaks in juvenile C. gigas, the role of OsHV-1 in oyster mortality is progressing. 相似文献
7.
Colin Nakata Clara Qualizza Mike MacKinnon Sylvie Renault 《Water, air, and soil pollution》2011,214(1-4):59-72
Methylibium petroleiphilum PM1, which is capable of degrading of methyl tert-butyl ether (MTBE), was immobilized in calcium alginate gel beads. Various applications were explored to increase the mechanical strength of these gel beads. The introduction of 0.3 mol/L calcium chloride into the crosslinking solution, 0.002 mol/L calcium chloride into the growth medium, and 0.2% polyethyleneimine (PEI) as chemical crosslinking agent increased the stability of the Ca-alginate gel beads under the operation conditions of the bioreactor. The degradation rates of MTBE by the immobilized cells in the bioreactor system operated in batch and continuous mode , respectively, were compared. A MTBE biodegradation rate of 5.79 mg/L·h was reached for over 400 h (50 batches), and the immobilized cells in the bioreactor removed >96% MTBE during 50 days of operation. Molecular analysis of the PM1 cells revealed that microbial growth occurred predominantly as microcolonies in the outer area of the beads during the first 20 days of operation. The results of this study show that a continuous-mode, fixed-bed bioreactor reactor coupled with PM1-immobilized cells is a promising technology for remediating MTBE-contaminated groundwater. 相似文献
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Gautier Camille Montarry Josselin Piriou Christophe Renault Lionel Porte Catherine Yvin Jean-Claude Nguema-Ona Eric Fournet Sylvain 《European journal of plant pathology / European Foundation for Plant Pathology》2021,161(3):553-563
European Journal of Plant Pathology - Cyst nematodes are sedentary endoparasites of plants which cause important economic losses worldwide. New nematode control measures are needed since the... 相似文献
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Katerina Nikolouli Hervé Colinet David Renault Thomas Enriquez Laurence Mouton Patricia Gibert Fabiana Sassu Carlos Cáceres Christian Stauffer Rui Pereira Kostas Bourtzis 《Journal of pest science》2018,91(2):489-503
Drosophila suzukii, a vinegar fly originated from Southeast Asia, has recently invaded western countries, and it has been recognized as an important threat of a wide variety of several commercial soft fruits. This review summarizes the current information about the biology and dispersal of D. suzukii and discusses the current status and prospects of control methods for the management of this pest. We highlight current knowledge and ongoing research on innovative environmental-friendly control methods with emphasis on the sterile insect technique (SIT) and the incompatible insect technique (IIT). SIT has been successfully used for the containment, suppression or even eradication of populations of insect pests. IIT has been proposed as a stand-alone tool or in conjunction with SIT for insect pest control. The principles of SIT and IIT are reviewed, and the potential value of each approach in the management of D. suzukii is analyzed. We thoroughly address the challenges of SIT and IIT, and we propose the use of SIT as a component of an area-wide integrated pest management approach to suppress D. suzukii populations. As a contingency plan, we suggest a promising alternative avenue through the combination of these two techniques, SIT/IIT, which has been developed and is currently being tested in open-field trials against Aedes mosquito populations. All the potential limiting factors that may render these methods ineffective, as well as the requirements that need to be fulfilled before their application, are discussed. 相似文献
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D. Renault 《Irrigation and Drainage Systems》1988,2(3):229-240
Surface irrigation rehabilitation in the South-East of France has shown success ever since the beginning of its development in the early 1980s. The gradual introduction of surface distributors, mechanized or automated has resulted in water savings and above all in decreased labor input. The distributors used are: gated pipes, layflat tubing, buried low pressure pipe and cablegation. They are used over an ever increasing area which attained about 1000 ha in 1987.Improvement is made in furrow head distribution as well as in field levelling, furrow flow evaluation, flow control at the head of the furrow and in the collective system.The effect of a modernized system and improved knowledge has been to make automated surface irrigation (particularly furrow irrigation) a modern application method in competition with techniques such as sprinklers and localized irrigation.Abbreviations ASAE
American Society of Agricultural Engineers
- CEMAGREF
Centre d'Etude du Machinisme Agricole du Génie Rural des Eaux et des Forêts, Division Irrigation, BP 5095, 34033 Montpellier et BP 31 Le Tholonet 13612 AIX
- ENGREF
Ecole Nationale du Génie Rural des Eaux et des Forêts, Département Maîtrise de l'Eau et Développement, BP 5093, 34033 Montpellier
- IRAT
Institute de Recherche en Agronomie Tropicale, Zolad Mini Parc Bt7, 34100 Montpellier
- P.V.C.
Polyvinyl chloride
- RNEDHA
Réseau National Expérimentation Démonstration secteur Hydraulique Agricole, BP 5095, 34033 Montpellier
- SCP
Société du Canal de Provence et d'Aménagement de la Région Provençale, BP 100, 13603 AIX en Provence 相似文献