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1.
Chu FL Pirastru L Popovic R Sleno L 《Journal of agricultural and food chemistry》2011,59(7):3004-3013
Carotenoids have potent antioxidant activity as well as therapeutic value, and their formation has been seen to be induced in algae by stress, including high-salt culture conditions. A differential profiling of carotenoids was conducted using a targeted metabolomics approach with accurate mass data generated by liquid chromatography-electrospray-time-of-flight (LC-ESI-TOF) mass spectrometry followed by postacquisition filtering based on isotope patterns and mass defects to detect carotenoids up-regulated in Scenedesmus sp. exposed to high-salt conditions. Algal cultures treated with high concentrations of sodium acetate or sodium chloride were found to cause an increase in various carotenoids. On the basis of differential analysis, astaxanthin and canthaxanthin increased upon salt treatment. Astaxanthin, in its free form and as fatty acid esters, was seen to increase in Scenedesmus sp. using accurate mass MS. A few other carotenoid compounds increased upon salt treatment, including echinenone and adonirubin, involved in the pathway of astaxanthin biosynthesis from β-carotene, as well as isomers of astaxanthin and canthaxanthin. A time course study of acetate treatment was done to observe the time-dependent up-regulation of carotenogenesis. 相似文献
2.
Journal of Plant Diseases and Protection - Serological and molecular methods were compared for the detection of Bean yellow mosaic virus (BYMV) in gladiolus plants. Plants showing mosaic symptoms... 相似文献
3.
Jan Mares Miroslava Palikova Radovan Kopp Stanislav Navratil & Jiri Pikula 《Aquaculture Research》2009,40(2):148-156
The present study evaluated the effect of naturally developing cyanobacteria on the composition of muscles of two commercially important freshwater fish species. Fish were exposed to cyanobacterial biomass including Microcystis aeruginosa and Microcystis ichthyoblabe for 4 weeks. Then, they were transferred to dechlorinated potable water without any cyanobacteria for another 4-week period, thus modelling their preparation for consumers. Samples of muscles were collected every week during exposure and subsequent stay in dechlorinated potable water. The cyanobacterial water bloom of 3.9–6 × 105 cells mL−1 (133–383 μg g−1 of total MC DW) induced statistically significant effects only in the content of fatty acids ( P <0.05; P <0.01) in the common carp ( Cyprinus carpio ), while all studied parameters including the content of dry matter and fat ( P <0.01), proteins ( P <0.05), fatty acid composition ( P <0.05; P <0.01) and some amino acids ( P <0.05) were affected in the silver carp ( Hypophthalmichthys molitrix ). This study has shown that cyanobacteria in the environment of commercially produced fish may decrease the dietetic value of fish muscles. 相似文献
4.
Summary A new cms source, ANN-5, was found in wild Helianthus annuus. This source showed high stability under different conditions in 1991 and 1992. All progenies from crosses of this source with several stable B-lines and restorer lines, which are homozygous for the gene which restores Leclercq's source of male sterility, were completely male sterile. Flower contained pistils and atrophied stamens. The cytological analysis showed that pollen mother cell degeneration took place in a premeiotic stage. 相似文献
5.
Radovan Kopp Miroslava Palíková Stanislav Navrátil Jan Mareš 《Fish physiology and biochemistry》2014,40(6):1651-1658
Cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. In the present study, we investigated the impact of cyanobacterial biomass on biochemical indices of common carp (Cyprinus carpio L., average weight of 246 ± 73 g) under laboratory conditions. The fish were fed a diet containing cyanobacterial biomass with microcystins in high concentration (0.4 mg/kg of fish weight and day) for 28 days. Statistical evaluation of the influence of the cyanobacterial biomass in food on the biochemical indices of the juvenile carp showed only minor differences. The activity of aspartate aminotransferase value and the urea concentration were significantly reduced compared to control group. The biochemical parameters of fish blood plasma significantly rose during the experiment in the control group as well as in the experimental group. This state was probably influenced by the environmental conditions and the fish diet. A significant rising value was established in calcium creatinine, total protein, phosphorus, lactate, urea and natrium. The present study demonstrates that the oral exposure of toxic cyanobacterial biomass has a minor influence on the biochemical indices of common carp and that the effect of other factors, e.g., nutrition is more visible. 相似文献
6.
Findoráková Lenka Šestinová Oľga Matik Marek Hančuľák Jozef Bureš Radovan 《Journal of Soils and Sediments》2022,22(9):2448-2458
Journal of Soils and Sediments - To evaluate the ecological risk, physico-chemical properties, and the impact of anthropogenic sources on permanent eighteen samples of grass vegetation soils (PGVS)... 相似文献
7.
Radovan Marinković 《Euphytica》1992,60(3):201-205
Summary Interactions of seven bio-morphological characteristics of sunflower (head diameter, number of flowers per head, number of seeds per head, 1,000-seed weight, volume weight, kernel content in seed, oil content) and their correlations with seed yield per plant were examined. The path-coefficient analysis provided information about direct and indirect effects of the examined characteristics on seed yield per plant. The correlation coefficients were positive and highly significant. The highest direct positive effect on seed yield per plant was exhibited by the weight of 1,000 seeds. Head diameter, volume weight and kernel content had a direct negative effect on seed yield per plant. 相似文献
8.
Radovan Kopp Jan Mare Miroslava Palíková Stanislav Navrátil Zdenk Kubíek rea Ziková Jana Hlávková & Ludk Bláha 《Aquaculture Research》2009,40(15):1683-1693
The aim of this study was to evaluate the dynamics of the blood plasma parameters and the content of microcystins in the tissues of common carp ( Cyprinus carpio L.) in relation to the toxic cyanobacterial water bloom. Fish (average body mass 2176±697 g) in the hypertrophic pond were exposed to natural water bloom (dominated by Planktothrix agardhii, Pseudanabaena limnetica and Limnothrix redekei ), which contained microcystins (concentration in biomass 20–181 μg g−1 dry wt, concentration in water 0.3–9.5 μg L−1 ). Biochemical parameters in fish blood plasma were analysed in 89 fish at 14-day intervals during the whole season (nine sampling periods). Our results demonstrated high variability and fluctuations in the investigated parameters. The content of microcystins and density of cyanobacterial cells correlated with some haematological indices as lipase, alanine–aminotransferase, albumin, magnesium and chlorides. The concentrations of microcystins in the muscle and liver of the fish (determined by high-performance liquid chromatography with mass spectrometer) were below the limit of detection during the monitored period [0.31 ng g−1 fresh weight (f.w.) for the liver and 0.13 ng g−1 f.w. for muscle]. Our study demonstrates that although known cyanobacterial toxin microcystins were not detected in the fish tissues, several biochemical parameters important for the fish physiology were modulated by natural cyanobacterial bloom. 相似文献
9.
Zuzana Drábková Johannes P. Schramel Radovan Kabeš 《Veterinary anaesthesia and analgesia》2018,45(1):73-77
Objective
To compare two methods of Bohr–Enghoff physiological dead space to tidal volume ratio (Vd/VtBohr–Enghoff) determination using a mixing chamber and an E-CAiOVX metabolic monitor.Study design
Prospective, clinical, method-comparison study.Animals
Twenty horses anaesthetized for elective orthopaedic procedures.Methods
Horses were anaesthetized with isoflurane in oxygen and the lungs were mechanically ventilated (Vt 15 ± 2 mL kg?1). Arterial blood was sampled to provide arterial partial pressure of carbon dioxide (PaCO2) for dead space calculation using a metabolic monitor. Mixed expired partial pressure of carbon dioxide (PēCO2) obtained from the custom-made mixing chamber was recorded at the time of arterial blood sampling. Dead space fraction was calculated using the Enghoff modification of the Bohr equation. Agreement between the methods was assessed by Bland–Altman test. A clinically acceptable error was defined to be ≤ 10%.Results
Forty-nine simultaneous Vd/VtBohr–Enghoff results were obtained. There was no clinically significant bias between the mixing chamber and E-CAiOVX. The limits of agreement were within a priori defined error (bias ± 95% limits of agreement: ?0.022 ± 0.078).Conclusions and clinical relevance
Acceptable agreement was found between the two methods. The E-CAiOVX metabolic monitor might be a suitable device for measuring Vd/VtBohr–Enghoff in anaesthetized horses. 相似文献10.
Metka Novak Andreja Urbanek Krajnc Ljerka Lah Neja Zupanec Nada Kraševec Mitja Križman Joerg Bohlmann Radovan Komel 《European Journal of Forest Research》2014,133(4):573-583
Among the most devastating pests of Norway spruce (Picea abies) are the European spruce bark beetle (Ips typographus) and the associated pathogenic blue-stain fungus Ceratocystis polonica. Following attack and colonization, the beetle and the fungus must cope with induced host chemical defenses, such as monoterpenes that are generally thought to be toxic to both symbionts. The goal of this study was to better understand the response of Norway spruce following C. polonica inoculation at low density that does not overwhelm the tree and to identify monoterpenes mobilized toward the fungus. We inoculated healthy mature trees and monitored monoterpene profiles 2, 3, and 5 months post-inoculation. We also exposed three different C. polonica strains to the most abundant or significantly up-regulated monoterpenes to determine differences in monoterpene toxicity and resistance among strains. Total monoterpene levels, including limonene, were increased at 2 and 3 months after inoculation and had dropped after 5 months. In in vitro assays, all monoterpenes were inhibitory to C. polonica. Limonene and β-pinene were the most potent inhibitors of fungal growth. The extent of inhibition varied between the three strains tested. These results showed a defense response of Norway spruce to C. polonica, in which limonene may play a critical role in inhibiting the spread of the fungus. We also showed that differences between strains of C. polonica must be taken into account when assessing the role of the fungus in this bark beetle–symbiont system. 相似文献