A Comparison of the Cutaneous Trunci Myocutaneous Flap and Latissimus Dorsi Myocutaneous Flap in the Dog

Latissimus dorsi and cutaneous trunci myocutaneous flaps of equal dimension and location were randomly elevated on opposite sides of the thorax in 10 dogs (group 1) and resutured to their respective bed. The procedure was repeated in four additional dogs (group 2); however, the short perforating branches of the thoracodorsal artery and vein were divided at the base of each cutaneous trunci myocutaneous flap, whereas the cutaneous pedicle and underlying cutaneous trunci muscle were divided in the latissimus dorsi myocutaneous flaps to determine subsequent skin survivability and the major source of circulation of each myocutaneous flap. There was little difference in the percentage of skin survival between the latissimus dorsi and cutaneous trunci myocutaneous flaps in group 1 dogs. Circulation to the "skin island" of group 2 latissimus dorsi myocutaneous flaps originated from intramuscular anastomotic connections between the major branch of the thoracodorsal artery entering the latissimus dorsi muscle and the proximal lateral intercostal arteries perforating the muscle. Ligation of the short perforating branches of the thoracodorsal artery resulted in partial skin necrosis in all group 2 cutaneous trunci myocutaneous flaps. Results from this study indicate that it is unnecessary to elevate the latissimus dorsi muscle for major skin flap elevation and survival. The thicker latissimus dorsi myocutaneous flap is more difficult to develop surgically and appears to have no clinical major advantage over the more mobile cutaneous trunci myocutaneous flap or the adjacent thoracodorsal axial pattern flap for closure of large skin defects within the radius of flap rotation.     

Forecasting Crop Damage by Nematodes: Nematode Population Dynamics1

Factors affecting the ease or difficulty of forecasting and modelling cyst, root-ectoparasitic and soil surface nematodes are mentioned. To relate pre-planting to post-harvest populations of cyst nematodes, a version of the logistic curve is derived from first principles and modified to take account of the injury done to root systems as numbers increase, and of eggs carried over to the following year. Egg hatch, when potato or other crops are grown, appears to be independent of population density. So also does root invasion over the population densities encountered in field plots. In the absence of enemies, population density is determined by competition for feeding sites induced by females in the root system, and by the size of the root system. Population oscillations about the equilibrium point are affected mainly by the proportion of the population that fails to hatch and the damage to the root system. The process of model building and the parameters required are outlined. The model includes hypotheses relating to intraspecific (genetic) competition and interspecific competition. The limitations of models and the need to make them stochastic are discussed. L'article fait état des possibilites et difficultés rencontrees dam la prévision et la modelisation des populations de nematodes a kystes, de nematodes ectoparasites des racines, et de nematodes de surface. Afin detablir la relation entre les populations de nematodes a kystes avant le semis et apres la recolte, il est recouru a la courbe logistique, derivee des principes fondamentaux. Celle-ci se trouve modifiee afin de tenir compte des degits croissants subis par les racines en fonction de I'augmentation des populations et de la survie d'une partie des ceufs d'une annee a I'autre. L'eclosion des ceufs semble être independante de la densite des populations dans les champs de pommes de terre et de toute autre culture. Ceci s'applique egalement a I'invasion des racines, dans le cas des densites de populations observees dans les parcelles experimentales. En I'absence dennemis, la densite est determinee par la competition pour L'acces aux sites de nutrition que les femelles preparent dans les racines, et par L'importance du systeme radiculaire. Les ecarts de population observes a partir dun equilibre sont principalement dus a la proportion de la population non eclose et aux deggts subis par les racines. I1 est fait etat d'une modelisation, basee sur certains parametres essentiels. Le modele repose sur L'hypothese d'une concurrence intraspecifique (genetique) et interspecifique. Les limites des modeles sont considerees, ainsi que la necessite de passer a une approche stochastique.     

栽培稻种间近等基因系杂种育性研究

 为了研究亚洲栽培稻与非洲栽培稻种间杂种不育的遗传基础，以亚洲栽培稻品种WAB56-104为测验种与来自种间回交组合WAB56-104/CG14//WAB56-104///WAB56-104的14个种间近等基因系测交，对亲本及杂种的花粉及小穗育性研究表明，这14个近等基因系带有来自非洲栽培稻品种CG14的3个互不等位的配子消除位点和2个互不等位的花粉不育基因位点, 其中至少有2个配子消除位点未曾报道过。     

Trivalent metal (Cr, Y, Rh, La, Pr, Gd) sorption in two acid soils and its consequences for bioremediation

The recent addition of trivalent metals to soil and their subsequent movement within the biosphere are of concern. For this reason, the sorption of chromium (Cr), yttrium (Y). rhodium (Rh), lanthanum (La), praseodymium (Pr) and gadolinium (Gd) in two contrasting acid soils has been determined. Except for Rh, the sorption of the other trivalent metals conformed well to the Langmuir equation with derived sorption parameters similar for all the trivalent species tested. Calculation of the buffer powers indicated that under both small (0·01 mmol kg^?1) and large (1 mmol kg^?1) trivalent metal soil loadings > 99·5% of the metals will be associated with the exchange phase with small quantities present in the bulk soil solution (<0·5%). It seems that the slight availability of metals within the bulk soil solution will slow the rate of trivalent metal bioremediation of contaminated sites.     

Comparison of responses to floret and seedling inoculation in wheat- Ustilago tritici and barley- U. nuda combinations

The floret and seedling methods for inoculating wheat and barley with Ustilago tritici and U. nuda, respectively, were optimized and then compared. Floret inoculation gave higher infection levels than did seedling inoculation, especially for wheat, but problems of plant mortality and standardization of tissue age were greater with the former method. Floret inoculation identified wheat and barley varieties exhibiting embryo resistance (class 1 or 2 resistance) or seedling resistance (class 3), but only class 3 wheat and barley varieties (and one class 2 wheat variety) were resistant to seedling inoculation. Using near-isogenic wheat lines expressing different levels of partial resistance to floret inoculation, positive correlations were obtained between percentage infection levels resulting from the two inoculation protocols. In addition, race-specific host-pathogen interactions such as hypersensitivity and partial smutting were expressed following both floret and seedling inoculation, indicating that these interactions operate in the developing plant. The potential value of the seedling inoculation method in fundamental and applied research into the cereal-loose smut interaction is discussed.     

Problems associated with potyviruses in potato certification-field inspection and serological testing1

In UK, the tobacco veinal necrosis strain of potato virus Y (PVY^N), potato virus A (PVA) and potato virus V (PVV) each occur in the field only in limited ranges of potato cultivars in which they mostly cause mild symptoms or even symptomless infection; little is known about incidence of strain C of PVY (PVY^C). The ordinary strain of PVY (PVY°), however, is widespread causing symptoms ranging in severity from very severe through to very mild, depending on cultivar sensitivity/tolerance. During field inspections, very mild potyvirus symptoms may be missed, so inspectors are trained to be particularly vigilant when examining problem cultivars which react in this way. PVA is almost invariably treated, along with PVX, as a mild kind of virus infection, but infections with PVY°, PVY^N and PVV are treated as severe with stricter tolerances being applied for them (especially for PVY^N) regardless of symptom severity. Wide variation within the same cultivar in the behaviour of variants within the PVY° strain group also sometimes causes difficulties in interpretation at inspection. To detect PVY, PVA and PVV in routine serological testing on potato certification samples, it is necessary to employ specific antisera to each of them. PVY^N-specific monoclonal antibodies can be used in ELISA to distinguish PVY^N from PVY°.     

Causes of preweaning mortality on a large intensive piggery

Causes of preweaning mortality were examined on a large intensive piggery. Diagnosis was made using comprehensive clinical histories combined with post-mortem data. Two thousand four hundred and twenty-six piglets were born in 238 litters. Pre-parturient and parturient losses were 2.9% and 5.4% of the total numbers of piglets born. Birth to weaning mortality was 11.3%. Among piglets born alive, overlaying was the most frequent cause of death (2.1%), followed by deaths due to diarrhoea (1.7%), anaemia (1.2%), savaging (1.1%) and losses of small weak piglets (0.9%). Most deaths, including stillbirths, were associated with below average birth weight, and two-thirds of all deaths of liveborn pigs occurred within 4 days of birth. Increased litter size resulted in decreased birth weights, an increased percentage of stillborn piglets and mummified foetuses, but duration of parturition was not affected. Stillborn piglets were born late in the litter and after a longer interval between pigs born (interpig interval). Over 70% of deaths due to overlay were in previously healthy piglets, but some were associated with illness of the sow (18%) or both sow and piglet (3%). Savaging was confined mainly to first parity sows and was responsible for 20% of all deaths in these litters. Sixty-two per cent of all piglets with a birth weight of less than 800g were stillborn or died before weaning compared with 18.7% mortality for all piglets. Anaemia in piglets was considered to be due to umbilical haemorrhage. Anaemic piglets had a 36% mortality to weaning compared with 10% for non-anaemic piglets.(ABSTRACT TRUNCATED AT 250 WORDS)     

The effect of seed pretreatment and sowing date on the nursery emergence of Sitka spruce (Picea sitchensis [Bong.] Carr.) seedlings

Sitka spruce seeds which had been: (1) untreated (U), (2) prechilled(P), or (3) prechilled and then redried and stored for 16 months(R), were sown into progressively warmer seed beds on four datesat monthly intervals from March to June. At each sowing date,samples of seed were germinated in the laboratory at optimum(20°C) and sub-optimum (10°C) temperatures. Prechillingand prechilling + redrying both increased the rate and totalamount of germination compared with untreated seed at each testtemperature. However in the nursery, the beneficial effectsof pretreating Sitka spruce seed were restricted to sowing inMarch and April when soil temperatures were sub-optimal forthe germination of untreated seeds. Pretreatment resulted inrapid seedling emergence at soil temperatures between 10°Cand 15°C, whereas untreated seed germinated slowly and seedlingemergence continued until high soil temperatures in June killedungerminated seeds. The pattern of seedling emergence from theredried seed was similar to that from the prechilled seed, exceptfor a lag in the start of seedling emergence probably due tothe extra time needed for redried seed to imbibe water in theseed beds. Thus redrying caused insignificant loss of seed performanceon the nursery. Seed sown in May and June experienced average temperatures closeto the optimum for germination of 20°C, however daily maximaregularly exceeded 35°C resulting in the cessation of seedlingemergence in all three seed treatments, and the loss of newlyemerged seedlings. Periods of warm weather from June onwardswere the main factors limiting the final number and size ofseedlings.