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Estrogens of human and animal origin that reach the aquatic environment may enter human or animal organism and act as endocrine disruptors.To investigate the persistency of estrogens in laboratory experiments, estrone respectively 17beta-estradiol were added to stream water sampled from river Spree in Berlin. The concentration of estrone and 17beta-estradiol was quantified using enzyme-immuno-assay. The estrone concentration decreased to less than 5 % of the starting concentration at storage temperature of 5 degrees C within 56 days and at storage temperature of 20 degrees C within 14 days. If the estrone were added to autoclaved stream water, no biodegradation was observed. Biodegradation was enhanced when activated sludge was added. Escherichia coli, Pseudomonas fluorenscens and Aeromonas hydrophila in monoculture did not degrade estrone in autoclaved stream water.The concentrations of 17beta-estradiol and estrone decreased similarly.The logistic function proved to be suitable to describe the course of time for the decrease of concentration.  相似文献   
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7-Hydroxy-5-oxo-5H-thieno[3,2-b]pyran-6-carboxanilides and -6-thio-carboxanilides represent a novel series of anthelmintic compounds, with broad-spectrum activity against important parasitic nematodes in sheep and dogs. In particular, an improved efficacy against Trichostrongylus colubriformis in sheep over the related 3-carbamoyl-4-hydroxycoumarins has been noted. New synthetic routes to the key intermediate, 7-hydroxythieno[3,2-b]pyran-5-one, have been developed.  相似文献   
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Intercropping or rotating of P‐efficient crop species which mobilize sparingly soluble P by their root exudates can have beneficial effects on growth and P uptake of P‐inefficient species. We aimed at studying the effect of intercropping or incorporating of crop residues of P‐efficient crops on the components of maize P‐uptake, i.e. the root‐system size and P influx (P‐uptake rate per unit root length). This was studied in 3 pot experiments in a low‐P sandy soil. In the first experiment, maize was intercropped with white lupine, sugar beet or oilseed rape, and with groundnut in the second experiment. In the third experiment, maize was grown after incorporating the crop residues of white lupine, sugar beet or oilseed rape. Maize growth and yield was strongly inhibited when intercropped with white lupine, sugar beet or oilseed rape, probably because of competition for nutrients. But with groundnut as the accompanying species, maize yield was increased by a factor of 3, mainly because of an enhanced P influx. Crop residues of oilseed rape and sugar beet increased the yield of maize by factors 2 and 1.6, respectively, because of a 3 and 2 times higher P uptake as compared to maize grown after maize without incorporation of crop residue. The reason for the higher maize P‐uptake after oilseed rape was an 11 times higher P influx as compared to maize without crop residues, and after sugar beet residues because of an enhanced root growth and a 4 times higher P influx. Lupine residues did not improve maize growth, mainly because of a low P influx, which was even less than that of maize grown without crop residues. The soil solution P concentration and calcium acetate lactate‐extractable P (CAL P) measured in this study did not reflect the P availability as indicated by the plants (P uptake, P influx). This indicates that other mechanisms such as P mobilization in the rhizosphere by root exudates or cell‐wall components were responsible for the increased P availability. These mechanisms need further investigation.  相似文献   
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Journal of Plant Diseases and Protection - In the original publication, the article title was incorrectly published as ‘Impact of leave infestation by herbivorous insects on the elemental...  相似文献   
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A 13-year-old mixed breed dog was presented with difficulties to urinate, blood in the urine and a nodule at the root of the penis. The penis was completely resected, and an osteosarcoma of the penile bone was diagnosed. Radiographically, there was no evidence to suggest tumours in other bones or distant metastases. The dog was euthanized as a consequence of tumour recurrence after 2 months. This is believed to be the first reported case of a primary osteosarcoma of the penile bone.  相似文献   
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To study adherence of Actinobacillus pleuropneumoniae to porcine lower respiratory epithelium, a cell culture model was developed using primary cultures of porcine lung epithelial cells (LEC). Adherence assays were performed and results were compared with data obtained with swine kidney cells (SK6). A. pleuropneumoniae efficiently adhered to LEC with up to 62 bacteria per cell after 2h of incubation. Reference strain of serotype 3 (R3) adhered better to LEC than reference strains of serotypes 1 (R1), 7 (R7) and 8 (R8). Overall the adherence to LEC was more rapid and up to 30-fold more efficient than adherence to SK6 cells. In search for the mechanism involved in the adherence event, we tested the effect of LPS which has previously been demonstrated to cause adherence of the pathogen to upper respiratory epithelium. Adherence assays with LPS transposon mutants demonstrated unaltered (mutant with modification in core/lipid A moiety) or even three-fold more adherence (mutants lacking O antigen) compared to the parent micro-organisms. Purified LPS of strains R1, R3, R7 and R8 did not inhibit adherence of R8 to LEC either, suggesting that LPS and particularly the O-antigen are not essential for adherence of A. pleuropneumoniae to LEC. The efficient, LPS-independent adherence of A. pleuropneumoniae to LEC cells indicates that A. pleuropneumoniae may carry different, cell type-specific adhesins and that primary cultures of lower respiratory epithelium are valuable infection models in studying A. pleuropneumoniae pathogenesis.  相似文献   
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The relationship between the physicochemical properties (molar volume, partition coefficient, and dissociation constant) of slow-acting systemic postemergence xenobiotics and their uptake and translocation to the sites of action was investigated using the nonlinear, dynamic simulation model ERMESSE. When the pKa was held constant at 4.0, the model enables the prediction of the uptake of a systemic xenobiotic as a function of its partition coefficient and molar volume. The model also considered the effects of the physicochemical properties of a systemic xenobiotic on its long-distance translocation within the vascular tissues. For instance, when the log Kow and pKa were held constant at 1.5 and 6.0, respectively, the model predicted a higher translocation rate (55%) for molecules with a small (e.g., MV = 100 cm3 mol−1) as opposed to a large (e.g., MV = 300 cm3 mol−1, 33%) molar volume. In addition, the theoretical predictions from the ERMESSE model showed that any xenobiotic with a molar volume not exceeding 300 cm3 mol−1 could provide an uptake ?50% and a translocation rate ?25% when its log Kow is between −0.5 and 2.5 and its pKa is between 0.0 and 8.0.  相似文献   
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