排序方式: 共有1条查询结果,搜索用时 15 毫秒
1
1.
Shantharaj Deepak Sekhar Shailasree Neerakkal Sujeeth Ramachandra K. Kini Axel Mithöfer Shekar H. Shetty 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(1):77-85
Proline/hydroxyproline-rich glycoprotein (P/HRGP) level in pearl millet genotypes resistant to downy mildew increase after
inoculation with the oomycete pathogen Sclerospora graminicola. Using purified P/HRGPs from pearl millet cell walls, polyclonal antibodies (Pab-P/HRGP) were raised in rabbit. Based on this
antiserum, an enzyme immunoassay was developed that displays a linearity detection range from 0.01 to 10 μg P/HRGP. Western
blot analysis, confirming the induction of three marker P/HRGPs in the infected resistant genotype, and immunocytochemical
studies on P/HRGP localization either in epidermal peelings or in suspension-cultured cells demonstrated the specificity of
the antiserum. Besides its characterization, Pab-P/HRGP was employed to screen various genotypes of pearl millet for fast,
sensitive and specific detection of induced P/HRGPs upon infections. The results presented are discussed with presumed importance
to downy mildew disease and the use of this new antiserum in pearl millet screening for disease resistance. 相似文献
1