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1.
Versatile physiological functions of the Nudix hydrolase family in berry development and stress response in grapevine 下载免费PDF全文
WANG Pei-pei WANG Zhao-ke GUAN Le Muhammad Salman HAIDER Maazullah NASIM YUAN Yong-bing LIU Geng-sen LENG Xiang-peng 《农业科学学报》2022,21(1):91-112
Nudix hydrolases are widely distributed across all classes of organisms and provide the potential capacity to hydrolyze a wide range of organic pyrophosphates. Although Nudix hydrolases are involved in plant detoxification processes in response to abiotic and biotic stresses, the biological functions of Nudix hydrolases remain largely unclear in grapevine.In the present study, a total of 25 putative grapevine Nudix hydrolases(VvNUDXs) were identified by bioinformatics analysis and classified int... 相似文献
2.
Ozen A Kalin R Yerlikaya H Seker I Cetinkaya B 《Journal of veterinary medical education》2004,31(3):281-288
A significant portion of the problems faced by veterinarians is related to communication. These problems are intensely experienced between veterinary practitioners and animal owners; yet no lectures or courses aiming to improve professional skills are available in the curriculum of veterinary schools in Turkey. In this study, all students currently enrolled at, and 1992 and 1998 graduates of, the Veterinary School of Firat University (VSFU), Turkey, were surveyed to evaluate the attitudes of veterinary students and graduates toward technical and professional skills. Data were collected from 581 students and 61 recent graduates via personal interviews with students and questionnaires mailed to graduates; information was obtained about participants' technical and professional skills. The overall response rate was 85%. The results show that learning about technical and professional skills is highly valued; competence and comfort in skill sets are associated with comfort in establishing communication with instructors. Positive correlations were also noted between feeling comfortable with and feeling competent in both skill sets. In conclusion, it appears to be essential to introduce courses addressing improvement of professional skills to the curriculum of veterinary schools in Turkey, given that a successful veterinarian profile requires feeling competent in and comfortable with both technical and professional skills. 相似文献
3.
Elshymaa A. Abdelnaby Ibrahim A. Emam Aya M. Fadl 《Reproduction in domestic animals》2021,56(5):764-774
This study aimed to determine the usefulness of colour and pulsed Doppler modes for the accurate diagnosis of donkeys suffering from subfertility to determine whether testicular vascularity assessment could be an indicator for sperm functionality. The study sample was composed of 10 male donkeys with normospermia (control group) and 10 donkeys with hypospermia. Animals underwent scrotal circumference measurement, testicular Doppler examination, seminal evaluation, blood sampling and hormonal assay. Semen volume and concentration were significantly (p ≤ .05) lower in the subfertile group (30.25 ± 1.22 ml and 89.44 ± 2.55 × 106/ml) as compared with the control group (82.76 ± 1.65 ml and 452.78 ± 1.25 × 106/ml), and total sperm/ejaculation was significantly (p ≤ .05) higher in the normal donkeys (28.30 ± 2.32 × 109/total ejaculated) as compared with the subfertile group. Intratesticular coloured area showed a marked decline in the hypospermic males. There was no significant difference between the two groups in testosterone level, although the normal group showed an increase in nitric oxide metabolites. Both Doppler indices of the three branches of the testicular artery were elevated significantly (p ≤ .05) in abnormal donkeys, whereas Doppler peak systolic and end-diastolic velocities were increased in the normal group. Male donkeys with subfertility demonstrated lower arterial vascularity parameters in the form of intratesticular coloured area and blood flow rate; therefore, the most optimal parameters for differentiating subfertile hypospermic from normospermic donkeys were found to be the two Doppler indices, velocities parameters, testicular blood flow rate and nitric oxide levels. 相似文献
4.
Chiemela Enyinnaya Chinma Yogeshini Ramakrishnan Muna Ilowefah Mat Hanis‐Syazwani Kharidah Muhammad 《Cereal Chemistry》2015,92(1):1-7
Cereal brans are functional ingredients with high nutritive value and enormous health properties. Cereal brans have not been fully utilized in food systems despite their health benefits. This review presents an overview on the physical, chemical, microbiological, functional, and sensory properties of cereal brans for possible comparisons and selection to enhance the utilization of this underutilized milling fraction. 相似文献
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Diallo IS Hewitson G Wright LL Kelly MA Rodwell BJ Corney BG 《Veterinary microbiology》2007,123(1-3):93-103
A multiplex real-time PCR was designed to detect and differentiate equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4). The PCR targets the glycoprotein B gene of EHV-1 and EHV-4. Primers and probes were specific to each equine herpesvirus type and can be used in monoplex or multiplex PCRs, allowing the differentiation of these two closely related members of the Alphaherpesvirinae. The two probes were minor-groove binding probes (MGB) labelled with 6-carboxy-fluorescein (FAM) and VIC for detection of EHV-1 and EHV-4, respectively. Ten EHV-1 isolates, six EHV-1 positive clinical samples, one EHV-1 reference strain (EHV-1.438/77), three EHV-4 positive clinical samples, two EHV-4 isolates and one EHV-4 reference strain (EHV-4 405/76) were included in this study. EHV-1 isolates, clinical samples and the reference strain reacted in the EHV-1 real-time PCR but not in the EHV-4 real-time PCR and similarly EHV-4 clinical samples, isolates and the reference strain were positive in the EHV-4 real-time PCR but not in the EHV-1 real-time PCR. Other herpesviruses, such as EHV-2, EHV-3 and EHV-5 were all negative when tested using the multiplex real-time PCR. When bacterial pathogens and opportunistic pathogens were tested in the multiplex real-time PCR they did not react with either system. The multiplex PCR was shown to be sensitive and specific and is a useful tool for detection and differentiation of EHV-1 and EHV-4 in a single reaction. A comprehensive equine herpesvirus disease investigation procedure used in our laboratory is also outlined. This procedure describes the combination of alphaherpesvirus multiplex real-time PCR along with existing gel-based PCRs described by other authors. 相似文献
7.
Ghanem YM Ibrahim el-SM Yamada S Matsumura T Osterrieder N Yamaguchi T Fukushi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):573-576
The pathogenicities of RacL11 and Kentucky D strains of equine herpesvirus 1 in the hamster infection model are different from those of Ab4p and the Japanese isolates. Virus genome restriction fragment length polymorphism analysis and sequence comparison of an intergenic region, glycoproteins and tegument genes showed higher conservation but with some strain-specific differences. These results indicate that point nucleotide differences in RacL11 and Kentucky D might be responsible for their pathogenicity in rodent models. 相似文献
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9.
Malik Muniba Abid Munir Haider Muhammad Saleem Zhai Ying Khan Muhammad Azmat Ullah Pappu Hanu R. 《植物病害和植物保护杂志》2023,130(1):23-33
Journal of Plant Diseases and Protection - Mastreviruses are an emerging group of viruses transmitted by leafhoppers and infect both monocot and dicot plants. Chickpea chlorotic dwarf virus... 相似文献
10.
Muhammad F. Bari 《Irrigation and Drainage Systems》1993,7(2):131-150
Flow conditions, which could not be explained, occurred in the stilling basin and outfall channel of the Feni Regulator sited at the western end of the Feni River closure dam. This regulator controls outflows from the upstream reservoir which supplies irrigation water to Muhuri Project in Bangladesh. Analysis of flood discharge data revealed that the design discharge for the structure was not exceeded; yet abnormal scour occurred in the outfall channel and the brick block rip-rap placed thereon was damaged. A model study was conducted to understand the causes of such unusual local erosion downstream of the stilling basin and to provide answers to two main questions: -Is potential scour serious in terms of the stability of the structure? -What protection measures could be taken to stabilize the scour at a safe level? Using a 1:30 scale model, the probable maximum scour was simulated, and the performance of alternative rip-rap designs including that of the existing one were examined. The results of this study supplemented by field scour data collected during subsequent flood seasons indicated that even if the flow rate through the regulator approaches the design flood discharge, the downstream scour is not likely to extend up to an elevation of ? 10.7 m, a scour level observed in the previous year at a lower discharge. It also showed that the existing rip-rap blocks were marginally undersized and consequently the rip-rap was prone to failure if flow conditions departed from uniform. A suitable method of scour protection downstream of the stilling basin at the regulator exit also evolved from the study. 相似文献