排序方式: 共有4条查询结果,搜索用时 0 毫秒
1
1.
Yuki Ichinose Rena Shimizu Yoko Ikeda Fumiko Taguchi Mizuri Marutani Takafumi Mukaihara Yoshishige Inagaki Kazuhiro Toyoda Tomonori Shiraishi 《Journal of General Plant Pathology》2003,69(4):244-249
The flagellins purified from Pseudomonas syringae pv. tabaci induce a hypersensitive reaction in nonhost tomato cells. To investigate the role of flagella and flagellin in the compatible interaction, we generated two types of flagella-defective mutant. The fliC mutant lost the fliC gene that encodes flagellin protein, whereas the fliD mutant lost the fliD gene that encodes HAP2-capping protein. The two mutants had markedly reduced ability to cause disease symptoms in tobacco leaves. Furthermore, propagation of the mutants in tobacco leaves was less than that in wild-type pv. tabaci. Compared to the inoculation with wild-type pv. tabaci, inoculation with the two mutants did not markedly induce the expression of typical defense response-related genes such as PAL and hsr203J. Complementation of each fliC and fliD gene to the corresponding deficient mutant restored motility and virulence. These results indicate that flagella of P. syringae pv. tabaci are indispensable organelles for complete virulence on host tobacco plants. 相似文献
2.
Lani E. Yakabe M. Malendia Maccree Padma Sudarshana Ali E. McClean Shane R. Parker William P. Wechter Gernot Presting Mizuri Marutani-Hert Daniel A. Kluepfel 《Journal of General Plant Pathology》2012,78(2):121-126
Novel PCR primers were developed to amplify a 243-bp fragment of an intergenic region between gene 5 and tms2 on the T-DNA of Agrobacterium tumefaciens biovar 1. These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence, and did
not generate extraneous bands, thus facilitating robust real-time PCR detection. 相似文献
3.
Mizuri Marutani Fumiko Taguchi Rena Shimizu Yoshishige Inagaki Kazuhiro Toyoda Tomonori Shiraishi Yuki Ichinose 《Journal of General Plant Pathology》2005,71(4):289-295
We have previously shown that flagellin of Pseudomonas syringae pv. tabaci is an elicitor that induces a hypersensitive reaction (HR) in nonhost tomato cells. Flagellin is the major HR elicitor produced by this pathogen, as shown by the inability of a flagellin-defective mutant, ΔfliC, to induce HR. Also, a ΔfliD mutant that secretes large amounts of monomer flagellins induces a strong HR in tomato. In this study, the possible involvement of an Hrp type III secretion system (TTSS) in flagellin-induced HR was investigated using flagella-defective mutants or Hrp TTSS-defective mutants. The hrcC gene encodes HrcC protein, which is required for Hrp pilus formation in the outer membrane. An hrcC mutation, introduced into the wild-type, ΔfliC, and ΔfliD mutants of P. syringae pv. tabaci did not affect swimming motility or flagellin secretion, whereas all ΔhrcC, ΔfliC, and ΔfliD mutants lost the ability to cause disease on host tobacco leaves. However, the ΔhrcC mutant and the ΔfliD/ΔhrcC double mutant were still able to induce HR cell death, expression of one of the defense-related genes hsr203J, and the generation of hydrogen peroxide in nonhost tomato cells. Thus, flagellin is required for both pathogenicity in host tobacco and HR in nonhost tomato. On the other hand, hrp TTSS is necessary for pathogenicity on host tobacco but is not indispensable to induce HR in nonhost tomato. These results clearly show that flagellin-induced HR is hrp-independent in tomato.The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AB049570 相似文献
4.
Hiroka Ito Satoshi Ito Tamotsu Matsui Tomomi Marutani 《Journal of Forest Research》2006,11(6):405-417
We investigated habitat segregation and patterns of species diversity of trees in relation to variations in fluvial and geomorphic
disturbances (erosion or sedimentation) along a longitudinal stream gradient from V-shaped valley to alluvial fan and between
valley and adjacent hill slopes in a warm-temperate mountainous riparian forest in Kyushu, southwestern Japan. We longitudinally
divided the riparian area into four geomorphic zones: V-shaped valley (VV-zone), upper fan (UF-zone), middle fan (MF-zone),
and lower fan (LF-zone). We surveyed the distribution of tree species (diameter at breast height ≥3 cm) in the four riparian
zones and in additional plots on hill slopes (SL-zone) representing the broader, nonriparian forest matrix. Detrended correspondence
analysis ordination demonstrated variations in species composition along the longitudinal stream gradient. Species guild analysis
based on the detection of the species preferred zone by a bootstrap method revealed a guild structure corresponding to each
geomorphic zone. The four riparian zones were differentiated from the SL-zone by having a low proportion of SL-guild species
and a high proportion of infrequent species that were characterized by deciduous leaf habit. The LF-zone was the most differentiated
and was characterized by low tree density and specialist species established on the flat and unstable soil surface created
by frequent deposition of sediment. The UF- and MF-zones were characterized by a high tree density and species richness (particularly
of infrequent species) established on the stable ground surface of a high alluvial terrace. Microsite heterogeneity produced
by channel formation may also maintain a high species diversity in the riparian zones. 相似文献
1