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排序方式: 共有471条查询结果,搜索用时 15 毫秒
1.
Kiriko Nakamura Kiyoshi Matsubara Hitoshi Watanabe Hisashi Kokubun Yoshihiro Ueda Naomi Oyama-Okubo Masayoshi Nakayama Toshio Ando 《Scientia Horticulturae》2006
In order to identify genetic resources for breeding fragrant petunias for use as bedding plants, volatile compounds released by day from the flowers of 40 commercial Petunia hybrida cultivars were analyzed using a solid-phase micro-extraction technique coupled with GC–MS. The three cultivars with solid deep-blue flowers that accumulate malvidin in corollas with high tissue pH were found to emit abundant iso-eugenol as the principal floral fragrance. Several other cultivars that emitted considerable amounts of methylbenzoate and/or benzylbenzoate from the flower were also identified. Association between the floral fragrance and the other floral traits such as floral anthocyanin composition and corolla-tissue pH was discussed. 相似文献
2.
Expression of HSP70 in response to heat-shock and its cDNA cloning from Mediterranean blue mussel 总被引:1,自引:0,他引:1
Haruhiko?ToyoharaEmail author Masatomi?Hosoi Isao?Hayashi Satoshi?Kubota Hisashi?Hashimoto Yoshihiro?Yokoyama 《Fisheries Science》2005,71(2):327-332
Expression of HSP70 in response to heat-shock was investigated at the protein and mRNA levels in Mediterranean blue mussel.
Western and Northern blot analyses revealed that HSP70 was expressed following heat-shock in the mantle at both protein and
mRNA levels, suggesting that gene expression of HSP70 is implicated in the cellular response to heat-shock stress in mussel.
It was then attempted to clone HSP70 cDNA in order to determine the primary structure of mussel HSP70. As a result, two full-length
cDNA encoding HSP70 were isolated from a cDNA library prepared from the heat-shocked mantle. The isolated cDNA consist of
single open reading frames of 2067 bp and 1911 bp which encode proteins of 689 amino acids and 637 amino acids, respectively.
Both HSP70 cDNA encode an ATPase do main, and a substrate-binding do main in addition to a Glu-Glu-Val-Asp (EEVD) peptide
motif that is specific for cytosolic HSP70. These findings suggest that the cDNA clones obtained in the present study encode
cytosolic HSP70. 相似文献
3.
Chihiro Kayo Seiji Hashimoto Atsunori Numata Masanori Hamada 《Journal of Wood Science》2011,57(3):234-240
We compared the greenhouse gas (GHG) emissions from a log pile (LP) to those from a sand compaction pile (SCP) and from cement
deep mixing (CDM) as measures against soil liquefaction, assuming that forest and waste management scenarios influence the
GHG (CO2, CH4, and N2O) balance of wood. We found little difference between the LP and SCP methods with respect to GHG emissions from fossil fuel
and limestone consumption. However, GHG emissions from the CDM method were seven times higher than emissions from the LP method.
In the GHG balance of wood, when the percentage of CH4 emissions from carbon in underground wood was lower than 3.3%, permanent storage in the log achieved greater reductions in
GHG emissions than using the waste log as fuel in place of coal or heavy oil. In order to obtain reductions in GHG emissions
by replacing SCPs or CDM with LPs, sustainable forest management with reforestation and prevention of CH4 emissions from the underground log are essential. Using reforestation, permanent storage of the log, no CH4 emission from the log, and using logging residues instead of coal, the LP can achieve reductions in GHG emissions of 121
tonnes of CO2 per 100 m2 of improvement area by replacing CDM. 相似文献
4.
5.
Kenzo Yoseda Kazuhisa Yamamoto Kimio Asami Masayuki Chimura Koji Hashimoto Shinichi Kosaka 《Aquaculture (Amsterdam, Netherlands)》2008,279(1-4):55-62
This study investigated the effect of different light intensities on feeding, growth and survival of early stage leopard coral grouper Plectropomus leopardus larvae. Four different light intensities (0, 500, 1000 and 3000 lx) were used and larvae were kept under constant light conditions from 0 day after hatching (DAH) to 5 DAH. The larvae were fed a small S-type of Thai strain rotifers at a density of 20 individuals/mL from 2 DAH. The number of rotifers in larval digestive organ and total length of larvae were examined at 3 h intervals between 04:00 and 22:00 h on 3 DAH, and thereafter at 6 h intervals until the end of the experiment (5 DAH). Four experimental trials of the larval rearing were repeated using by 60 kL mass-scale rearing tanks. The results indicate that coral grouper larvae are visual feeders and their food intake increases with increasing light intensity. Food intake of larvae reared at 3000 lx was significantly higher than those reared at 0–1000 lx on 3 DAH despite being the first-feeding day (P < 0.01). On 4 DAH, total length of larvae reared at 3000 lx was significantly larger than those reared at the lower light intensities (0, 500 and 1000 lx), and thereafter light intensity significantly influenced larval feeding and growth until the end of the experiment. Survival on 5 DAH did not show a significant difference between light intensities, but survival rate at 3000 lx and 1000 lx had a tendency to be higher than those reared at the lower light intensities (0 and 500 lx). In contrast, larvae reared at 0 lx exhibited stagnant and/or negative growth. These results indicate that light intensity is significantly the factor affecting larval feeding, growth, and survival in coral grouper larvae under the rearing conditions. 相似文献
6.
Yuji Sumii Kentaro Kamiya Takehiko Nakamura Kenta Tanaka Takumi Kaji Junya Mukomura Naoyuki Kotoku Masayoshi Arai 《Marine drugs》2022,20(2)
The current tuberculosis treatment regimen is long and complex, and its failure leads to relapse and emergence of drug resistance. One of the major reasons underlying the extended chemotherapeutic regimen is the ability of Mycobacterium tuberculosis to attain a dormant state. Therefore, the identification of new lead compounds with chemical structures different from those of conventional anti-tuberculosis drugs is essential. The compound 3-(phenethylamino)demethyl(oxy)aaptamine (PDOA, 1), isolated from marine sponge of Aaptos sp., is known as an anti-dormant mycobacterial substance, and has been reported to be effective against the drug resistant strains of M. tuberculosis. However, its target protein still remains unclear. This study aims to clarify the structure–activity relationship of 1 using 15 synthetic analogues, in order to prepare a probe molecule for detecting the target protein of 1. We succeeded in creating the compound 15 with a photoaffinity group that retained antimicrobial activity, which proved to be a suitable probe molecule for identifying the target protein of 1. 相似文献
7.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
8.
9.
10.
Katamoto H Yamada Y Nishizaki S Hashimoto T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):1001-1002
The influence of the season on serum vitamin A, E and beta-carotene concentrations was studied in Japanese Black breeding cattle. Blood samples were collected from 13 cows once a month for a year to determine the serum vitamin concentrations. Mean serum concentrations (+/- SE) of retinol, alpha-tocopherol and beta-carotene were 21.3 +/- 0.5 microg/dl (range 9.9 to 44.9), 215.9 +/- 8.0 microg/dl (range 59.7 to 551.6) and 67.3 +/- 5.1 microg/dl (range 10.6 to 425.2), respectively. All these concentrations were lower than the recommended levels. Serum retinol and alpha-tocopherol concentrations in summer were lower than those in winter. These results suggest that the vitamin A, E and beta-carotene status of Japanese Black breeding cows is inadequate, and feeding high quality forages or dietary vitamin supplementation during summer should be recommended. 相似文献