Comprehensive transcriptional profiling and functional study of lymphangiogenic growth factor in placentome of early-stage pregnant water buffalo

The aim of the present study was to evaluate the expression and localization of lymphangiogenic factors (VEGF-C and VEGF-D), their receptor (VEGFR3) and lymphatic endothelial marker (LYVE1) in buffalo placenta during early pregnancy [EP], and to investigate the functional role of lymphangiogenic growth factors in placental lymphangiogenesis. The mRNA and protein expression of VEGF-C, VEGF-D, their receptor VEGFR3 and LYVE1 showed significant expression in EP1 (29–42 days) and EP2 stages (51–82 days) both in caruncle (maternal part) and cotyledon (foetal part) of the buffalo placenta. Immunoreactivity of VEGF-C, VEGF-D and LYVE1 was observed around the endometrial gland, in lymphatics and trophoblast cells, whereas VEGFR3 mainly localized in lymphatics of the caruncle and cotyledons. Cultured trophoblast cells were treated with VEGF-C/VEGF-D (50, 100 and 150 ng/ml) and combined doses of VEGF-C and VEGF-D (150 ng/ml) each for different time durations (24, 48 and 72 h). The mRNA expression of LYVE1 and PCNA was significantly (p < .001) upregulated with VEGF-C and VEGF-D and combined treatment (@150 ng/ml), as well as significantly downregulating Caspase-3 at 48 and 72 h. Thus, the present study provides evidence that lymphangiogenic factors are expressed in buffalo placental compartments and they may play a significant role in the regulation of placental function in water buffaloes.     

Distribution pattern and conservation of threatened medicinal and aromatic plants of Central Himalaya,India

A study was conducted to examine the distribution pattern of four rhizomatous medicinal and aromatic plant species (MAPs) viz., Angelica glauca, Pleurospermum angelicoides, Rheum emodi and Arne- bia benthamii in different forest stands in Central Himalaya. Results show that A. glauca and P. angelicoides had a higher (50%) frequency at Chipkoan, Garpak and Phagati forest, R. emodi had a higher (60%) frequency at Rishikund, Suki and Himtoli, and A. benthamii had a higher (70%) frequency at Suki and Khambdhar The densities of A. glauca (0.6 plants·m -2 ) and P. angelicoides (0.5 plants·m -2 ) were higher at Chipkoan and Garpak sites than at other micro-sites, while densities of R. emodi (0.8 plants·m -2 ) and A. benthamii (1.0 plants·m -2 ) were higher at Suki and Khambdhar sites. A. glauca had highest total basal covers (TBC) (1.2 cm 2 ·m -2 ) at Chipkoan, P. angelicoides had highest TBC (0.92 cm 2 ·m -2) at Lati kharak site, A. benthamii had the highest TBC (6.48 cm 2 ·m -2 ) atKhambdhar, and R. emodi had highest TBC (4.53 cm 2 ·m -2 ) at Rishikund. For the four studied species, A. glauca showed a contagious distribution, P. angelicoides and R. emodi showed the random and A. benthamii showed the regular type of distribution.     

Floral Diversity and Distribution in the High Altitude Cold Desert of Ladakh,India

This study deals with the floral diversity and distribution patterns in plant species in relation to environmental gradients in the high altitude cold desert of Ladakh, India. An extensive literature survey and field explorations were made across the different parts of Ladakh in order to create a compilation of vascular plant species. For the quantification of vegetation, a total of eight habitat types were identified and sampled using the point intercept method. In all, 647 species of vascular plants belonging to 58 families and 243 genera were documented. Asteraceae (89 species), Poaceae (66 species), Brassicaceae (54 species), and Fabaceae (43 species) were the dominant families. Astragalus was the most dominant genus as it obtained 24 species, followed by Polygonum (18 species), Artemisia (15 species), and Potentilla (14 species). Among the habitats, riverine beds contained the highest frequency of plant species, followed by narrow gorges and tablelands. The crags and ridges had the lowest frequency of plants. Relatively high water and moisture availability may be the cause of the high frequency of plant species in riverine beds and narrow gorges. The vegetation composition was influenced by elevation; however, the intensity of change reduced at >4500-m elevation. The conservation and management of high altitude cold desert vegetation are discussed based on field observations and findings.     

Anthropogenic disturbances and status of forest and wildlife in the dry deciduous forests of Chhattisgarh state in India

The advent of modern forces and the changes in socioeconomic patterns of forest dwellers have increased the pressures on the forests. In order to mitigate such pressures and also to protect the forests and wildlife the model of protected areas networks has shifted and enhanced such pressures in the unprotected natural forests due to several reasons. Being a low profile category of protected status and continuous human settlements, the present study highlights the case of dry deciduous forests of Sarguja district of Chhattisgarh state of India. The major objectives of this study were to quantify the status of forests and wildlife and also to determine the extent of anthropogenic disturbances faced by the dry deciduous forests of central India. Transect and silent drive count methods were used for sampling wildlife and quadrat method was used for sampling vegetation. Besides, the local uses of various forest produces were also studied in view of understanding the people dependency on forests. The forest vegetation, in the study area, was pre-dominated by Shorea robusta, which had Madhuca indica, Diospyrus melanoxylon and Buchnania lanzan as the major companion species. The forest had either the high girth class mature tree species or the saplings. The low vegetation cover and density were due to the high anthropogenic pressures mainly in the form of heavy livestock grazing and collection of ethnobotanically important species. The study though reveals that the area is not rich in wildlife and the forest is fragmented, the area still supports some important species, which include many rare and endangered plants and animals. The findings of this study have been discussed in view of the management and conservation of the forest and wildlife in the dry deciduous forests.     

Effects of harvesting on productivity of bay leaf tree （Cinnamomum tamala Nees ＆ Eberm）： Case from Udayapur district of Nepal

Cultivation of bay leaves （Cinnamomum tamalaNees ＆ Eberm） to fulfil household income needs is a long established practice in Udayapur district of Nepal. The practices adopted by farmers for bay leaf harvesting have not, however, been validated by scientific investigation for their sustainability. To investigate the impacts of harvesting on the yield of branch, leaves and biomass of leaves, a two-year research project was conducted in farm fields at Kopche village of Routa VDC in Udayapur district, Nepal. Four different harvesting treatments, the orien-tation and the order of branches were taken as independent variables to test their effects on number of branches, leaves and biomass of leaves. Orientation, harvesting treatments and order of branches had a significant effect on the number of branches, but not on the number of leaves or biomass （fresh and dry weight） of leaves in the year of harvest. Between two consecutive harvests there was no significant difference in the num-ber of branches, leaves or biomass. Lower two-thirds portion of the trees produced the largest number of leaves and branches of the fourth order in both years. Therefore, lower two-thirds portion of the trees were suitable for harvesting. Our findings support farmer experience that no change in productivity of leaves is observed when harvesting each year. For long term sustainability, harvesting should be conducted without debarking of trees or damage to branches. Our findings could be extrapolated to and tested in other areas with different access and user rights where the rota-tion for harvest is fixed or regulated without research evidence.     

Effect of home processing on ascorbic acid and β-carotene content of spinach (Spinacia oleracia) and amaranth (Amaranthus tricolor) leaves

The present investigation was conducted to study the concentration of ascorbic acid and -carotene in spinach and amaranth leaves as affected by various domestic processing and cooking methods which included storage of leaves in polythene bags or without packing for 24 and 48 hours in refrigerator at 5 °C; at 30 °C in polythene bags; drying (sun and oven); blanching (5, 10, 15 min); open pan and pressure cooking. Ascorbic acid content of fresh leaves was 624.1 to 629.0 mg and -carotene content was 35.3 to 53.1 mg/100 g dry weight. The percent loss of ascorbic acid ranged from 1.1 to 6.3 and 55.3 to 65.9 while lower losses (0.0 to 1.3 and 1.5 to 2.1) of -carotene were observed in leaves stored in refrigerator and at 30 °C, respectively. A markedly greater reduction in ascorbic acid and -carotene was observed in dried, blanched and cooked leaves. The study recommended the storage of leaves in refrigerator, drying in oven, blanching for shorter time and cooking in pressure cooker for better retention of these two vitamins.     

Effect of home processing and storage on ascorbic acid and β-carotene content of bathua (Chenopodium album) and fenugreek (Trigonella foenum graecum) leaves

The present investigation was conducted to study the effect of selected processing and storage methods on the concentration of ascorbic acid and β-carotene in Bathua and fenugreek, leaves. Methods included storage of leaves with or without polythene bags for 24 and 48 h in a refrigerator at 5°C; at 30°C in polythene bags; drying (sun and oven); blanching (5, 10, 15 min); open pan and pressure cooking. Ascorbic acid content of fresh leaves was 220.97 to 377.65 mg and β-carotene content was 19.00 to 24.64 mg/100 g, DW. The percent loss of ascorbic acid ranged from 2.03 to 8.77 and 45.15 to 66.9 while lower losses (0.0 to 1.75 and 1.63 to 2.84) of β-carotene were observed in leaves, stored in, the refrigerator and at 30°C, respectively. A markedly greater reduction in ascorbic acid and β-carotene was observed in dried, blanched and cooked leaves. The study data suggest that storage of leaves in refrigeration, drying in oven, blanching for a short time and cooking in a pressure cooker results in better retention of these two vitamins.     

Effect of domestic processing and cooking on selected antinutrient contents of some green leafy vegetables

Three vegetables, 5 roots and tubers, 23 fruits and 3 stored products collected and consumed by tribals of Andhra Pradesh, India were analyzed for total carotenoids (TC) and Beta carotene (BC) contents using High Performance Liquid Chromatography (HPLC). The vitamin A activity expressed as retinol equivalents (RE) was calculated based on the in vivo conversion factor given by WHO. The results of the study indicated that the vegetables, roots and tubers analyzed showed negligible vitamin A activity with TC and BC content ranging from 0.01 to 0.61 and 0.14 to 0.23 mg%, respectively. Among the fruits, Canthium parviflorum contained very high TC (9.51 mg%) and BC (6.10 mg%) contents, and the edible orange color fresh rind portion analyzed had the highest (6.8 mg%) BC content. Other fruits contained negligible content of TC (0.0015 to 1.32 mg%) and BC (0.004 to 0.49 mg%). The edible pulp portion of the fruit (Diosphros melanoxylon contained no BC but the rind portion analyzed fresh and dried had BC) 0.79 and 2.17 mg%, respectively. Tribals were found to have distinct attitudes and beliefs towards food they use or avoid in certain physiological conditions.