绵羊瘤胃液中固氮菌的分离及其特性的研究

由两头绵羊的瘤胃液中分离到三株具有固氮能力的细菌,可于无氮培养基中生长繁殖,其中一株为需氧固氮,固氮能力较强,另两株为厌氧固氮,固氮能力较弱,鉴于它们在瘤胃液中存在数量较多,认为是绵羊瘤胃中一个正常的菌群,从而为瘤胃菌群增加了一个新的类群,并进行了生化鉴定及生长特性的研究。     

In situ hybridization analysis of ovarian prostaglandin endoperoxide synthase mRNA throughout the periovulatory period of the ewe.

Cellular alterations in level of expression of mRNA encoding for prostaglandin endoperoxide synthase were quantified within ovarian tissues of sheep obtained before, during and after induction of the preovulatory surge of LH and ovulation with LHRH. This was accomplished by isotopic in situ hybridization using a selective cRNA probe to ovine prostaglandin endoperoxide synthase mRNA. A significant elevation in mRNA was detected within the theca interna of the preovulatory follicle at 8, 16 and 24 hr following administration of LHRH. Very close to the time of ovulation (ie., at 24 hr post-LHRH) a marked rise in mRNA was observed in association with epithelial cells covering the apical surface of the follicle. Ovarian cyclooxygenase metabolites of arachidonic acid produced during the ovulatory process in the ewe originate within the thecal layer and germinal epithelium of the follicle destined to ovulate.     

草鱼多联佐剂疫苗的研制——Ⅰ. 疫苗的制备及免疫原性的研究

用草鱼出血病、肠炎病、赤皮病和烂鳃病的病原与PHA按一定比例混合制成联合疫苗,注射当年草鱼,安全性好,对上述各病的免疫保护率(%)依次为93.3±1.5,77.43±12.25,68.2±4.66和B6±2.55;免疫鱼血清抗体效价分别为1047.7±561.2,354.6±120.5,57.6±14.3和2379.2±37B.9;外周血ANAE阳性淋巴细胞的百分率(‰)为63.2±3.4.63.4±l.8,60.2±1.79和63±2.74,与来免疫鱼有极其显著的差异(P<0.01)。     

Protection efficiency assessment and quality of coastal shelterbelt for Dongshan Island at the coastal section scale

The coastal shelter forest in China is under threat of destruction and degradation because of the impact of human activities. Protection efficiency assessment of the coastal shelterbelt is an important component of shelter-forest remediation planning and sustainable management. In this study, a protection efficiency index (PEI) model was established using the projection pursuit method to assess the protective quality of the coastal shelter forest at the coastal section scale of Dongshan Island, China. Three criteria were used, including forest stand structure, forest belt structure, and windbreak effect; each criterion further comprised multiple factors. Based on survey data of 31 plots in the coastal shelter forest of Dongshan Island, we calculated PEI values using a projection of a pursuit model. The result showed 64.5 % of the PEIs fell at or below the middle level, which can indicate the status of the coastal shelterbelt is unsatisfactory. To further explore whether the different bays and land use types create significant differences in PEIs and evaluation indices, we used an ANOVA to test the influence of various bays and forms of land use on coastal shelterbelts. The results showed that PEI and most of the indices differed significantly by bay; mean tree height, mean DBH, mean crown width, stand density, vegetation coverage, and wind velocity reduction differed significantly by land use. Therefore, relevant measures for different locations, bays and surrounding land use can be proposed to improve the existing conditions of the coastal shelterbelt. The results of this study provide a theoretical and technical framework for future changes and sustainable management of coastal shelterbelt on Dongshan Island.     

The organic acid profiles in the feces of pigs at Brachyspira hyodysenteriae- or B. pilosicoli-positive farms

We observed a significant difference in the organic acid profile of diarrheal feces between pigs infected with and free from pathogenic spirochetes. Diarrhea and loose feces were collected from growing pigs, held at 15 different commercial farms. A total of 106 samples were measured for organic acid concentration by HPLC and were checked for the presence of B. hyodysenteriae and B. pilosicoli by PCR. B. hyodysenteriae was detected in 3 samples collected from one farm. B. pilosicoli was detected in 5 samples collected from another farm. Lower concentrations of iso-butyrate and iso-valerate were likely associated with development of pathogenic spirochete infection.     

Marek's disease resistant/susceptible MHC haplotypes in Xiayan chickens identified on the basis of BLB2 PCR-RFLP and BLB2/BF2 sequence analyses

1. The aim was to analyse the variability of the BLB2/BF2 genes of Xiayan chickens to identify homozygous birds with resistance or susceptibility to Marek's disease (MD).

2. The experiment used two lines: birds from a common line were divided into Group A (unvaccinated) and Group B (vaccinated with herpesvirus of turkeys (HVT)); and birds from an MD-resistant line were divided into Group C (unvaccinated) and Group D (vaccinated with HVT). They were challenged intra-abdominally with Marek's disease virus (MDV) and genomic DNA extracted from peripheral blood lymphocytes so that polymorphism of the BLB2/BF2 genes could be analysed using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and nucleotide sequence analysis.

3. A 374-bp fragment of the BLB2 gene was amplified from the samples and, after digesting with restriction enzymes Alu I, Cai I, Cfr I, Hin1 I, Hinf I and Rsa I for RFLP analysis, the 6 electrophoretic patterns were analysed. Seven homozygous genotypes were found and used tentatively to identify alleles of the BLB2 gene.

4. A 765-bp fragment of the BF2 gene was amplified from the 7 samples for cloning and sequencing.

5. Six homozygous birds were confirmed from the sequenced BLB2/BF2 gene. Four birds were resistant to MD. Three birds had identical nucleotide sequences and were highly homologous with MHC haplotype B⁶, which is MD resistant. One bird had high homology with the highly MD-resistant B²¹ haplotype, and two birds were susceptible and highly homologous to the B¹⁹ haplotype, which is highly MD susceptible.