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1.
Seasonal Changes in the Milt Quality of Waigieu Seaperch,Psammoperca waigiensis: Implications for Artificial Propagation
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Milt quality of Waigieu seaperch was sampled at the beginning, the middle, and the end of the spawning season to assess if the sampling season has an effect on milt quality parameters. The milt volume and total sperm production were higher at the middle of the spawning season while the sperm concentration in milt was significantly higher at the beginning and the end of the spawning season. Sperm morphology and the major parameters of seminal plasma (pH, total protein, Mg2+, or Ca2+ concentrations) did not differ throughout the spawning season. The concentrations of Na+ and the osmolality increased throughout the spawning season. The percentage of motile cells did not differ during the spawning season while the duration of sperm motility (swimming duration) and velocity (swimming speed) were significantly different during the spawning season. The fertilization rate (75.7 ± 6.8%) and hatching rate (56.5 ± 3.1%) at the middle of the spawning season were higher than at the beginning and end periods. Results indicated that milt quality parameters and fertility success in the middle of the spawning season were higher compared to earlier and later sampling dates. Thus, we recommend collecting milt during this time for maximal fertilization and hatching success. 相似文献
2.
描述了平背树螽属2新种,定名为双刺平背树螽(Isopsera bicuspidota Yang etKang)和广西平背树螽(I.guangziensis Yang et Kang)。 相似文献
3.
以 1 7β-雌二醇 -黄素腺嘌呤二核苷酸结合物为标记物 ,以葡萄糖氧化酶为全酶 ,建立了 1 7β-雌二醇脱辅基酶再激活免疫测定系统 (1 7β- E2 - ARIS) ,完成了两条标准曲线的制作 ,通过 logit代换法对两条标准曲线进行了直线回归。两条标准曲线的拟合度 (r)分别为0 .996 7* * 和 0 .9991 * * ,灵敏度分别为 1 1 .5 4pg· m L- 1 (2 . 31 pg·孔 - 1 )和 1 4.88pg·m L- 1 (2 . 98pg·孔 - 1 ) ,平均变异系数(CV% )分别为 4.6 4%和 9.34% ,测定范围为 31 .2 5~ 1 0 0 0 pg·m L- 1 。 相似文献
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以17β-雌二醇-6-人血清白蛋白对新西兰公兔进行主动免疫,用RIA检测抗体滴度和17β-雌二醇及睾酮浓度,并检测睾丸重量和间质细胞面积。结果,试验组各兔均不同程度地产生了17β-雌二醇抗体,其血浆17β-雌二醇浓度极显著地低于对照组〔(20.3±21.6)ng/L,(167.7±49.6)ng/L,P<0.001〕,睾酮浓度极显著地高于对照组〔(7.3±7.1)μg/L,(0.52±0.29)μg/L,P<0.01〕。每侧睾丸重量和间质细胞面积均极显著地大于对照组〔(3.94±0.93)g和(252.25±85.78)μm2,(2.98±0.72)g和(161.79±36.45)μm2,P<0.01和P<0.001〕。提示17β-雌二醇主动免疫可望成为提高雄性动物生育力的有效途径之一 相似文献
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Acquired resistance triggered by elicitins in tobacco and other plants 总被引:17,自引:0,他引:17
Philippe Bonnet Eva Bourdon Michel Ponchet Jean -Pierre Blein Pierre Ricci 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(2):181-192
Elicitins are a family of proteins excreted byPhytophthora spp. They exhibit high sequence homology but large net charge differences. They induce necrosis in tobacco plants which then become resistant to the tobacco pathogenPhytophthora parasitica var.nicotianae. In stem-treated plants, resistance was not restricted to the site of elicitin application, but could be demonstrated by petiole inoculation at all levels on the stem. Resistance was already maximum after two days and lasted for at least two weeks. It was effective not only towardsP. p. var.nicotianae infection, but also against the unrelated pathogenSclerotinia sclerotiorum. In contrast to dichloroisonicotinic acid, an artificial inducer of systemic acquired resistance, which was increasingly effective with doses ranging from 0.25 to 5mole per plant, the basic elicitin cryptogein exhibited a threshold effect, inducing near total resistance and extensive leaf necrosis above 0.1 nmole per plant. Between 1 and 5 nmole, acidic elicitins (capsicein and parasiticein) protected tobacco plants with hardly any necrotic symptom. Elicitins exhibited similar effects in various tobacco cultivars andNicotiana species, although with quantitative differences, but induced neither necrosis nor protection in other SolanaceÆ (tomato, petunia and pepper). Among 24 additional species tested belonging to 18 botanical families, only some BrassicaceÆ, noticeably rape, exhibited symptoms in response to elicitins, in a cultivar-specific manner. Elicitins appear to be natural specific triggers for systemic acquired resistance and provide a tool for unraveling the mechanisms leading to its establishment.Abbreviations AR
acquired resistance
- HR
hypersensitive response
- INA
2,6-dichloroisonicotinic acid
- Ppn
Phytophthora parasitica var.nicotianae
- SAR
systemic acquired resistance 相似文献
9.
Alba Marina Cotes Philippe Lepoivre Jean Semal 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(5):497-506
An experimental protocol consisting in the colonisation of pregerminated bean seeds dressed withTrichoderma sp. was used in order to study the mechanisms correlated with the protective effect againstPythium splendens. Seed dressed with TH-11 (T. koningii) for 24 h presented a higher protective effect and a higher level of seed colonisation as compared to those dressed with TH-13 (T. longibranchiatum). The levels of seed coat colonisation by TH-11 and TH-13 was shown to be correlated with the carboxymethylcellulase activity, as measured in the seed coats retreived from germinating dressed bean seeds. The seed coat colonisation was also associated with an increased activities of endo-1,3--glucanase and endochitinase measured in seed extracts, and an inhibitory effect of seed extracts onPythium sporangia germination. Pretreatment of TH-13-dressed seeds with a commercial cellulase improved all parameters mentioned above, thus suggesting a role of cellulase activity in the colonisation process and the linked protective effect. The possible role of hydrolytic enzymes in the protective effects is discussed. 相似文献
10.
1. The performance of six commercial stocks of laying hens fed on a diet composed of home‐produced foodstuffs, not usually used for human consumption, was compared with that of birds fed on a conventional diet.
2. The experimental diet was calculated to be low in energy and methionine and high in fibre by conventional standards. Birds ate up to 12% more of this diet, but the calculated intakes of energy and methionine were less than those of the corresponding controls.
3. White stocks fed on the experimental diet produced seven eggs less per bird than controls while the production of brown stocks was essentially unaffected.
4. The gross efficiency of energy utilisation for egg production was better with the experimental diet. 相似文献