A dynamic mass-balance model for marine protected areas

A modified Ecosim model was used to investigate the impact of establishing marine protected areas (MPAs) in ecosystems defined by existing Ecopath models. The impact of MPAs of various sizes was simulated, and changes in biomass and catch over a range of years observed. The response of biomass and catch to MPA size depended on the time period examined. For some ecosystem groups, the initial response was negative, but for all groups there were increases after 10 years. The greater the biomass exchange rate across the MPA boundary, the larger the MPA required to increase biomass levels. Within the range of exchange rates simulated, the maximum increases in catch and overall biomass levels were reached when 20% of the system was protected.     

A COMPARATIVE STUDY OF PAPER AND OIL AS CARRIERS OF CHROMIUM SESQUIOXIDE ADMINISTERED TO GRAZING STEERS TO DETERMINE THEIR FAECAL OUTPUT

Two steers excreting similar quantities of organic matter daily were grazed at pasture during the summer of 1961. The four experiments each involved a preliminary period of five days dosing with Cr₂O₃ to reach a fairly constant excretion. The steers received a daily dose of Cr₂O₃ either in oil suspension or in shredded paper. The carriers and steers were interchanged for each trial. For two of the trials each steer in turn was harnessed every other day and all its dung collected in a bag. Total faecal excretion of organic matter was measured from the bagged steer and estimated from the Cr₂O₃ concentration in a composite sample made up from all dung pats voided during the ten-day collection period. In the other two trials the steers were not harnessed and each dung pat was separately sampled and analysed for Cr₂O₃ in the organic matter. The average recovery of Cr₂O₃ in the first two trials was 99·1 (oil) or 94·4% (paper). Calculating the faecal organic matter excretion from the concentration of Cr₂O₃ in the faeces and the quantity given daily overestimated it by 3·5 to 8·0%. There was no significant difference between shredded paper and oil as carriers of Cr₂O₃ for estimating the quantity of organic matter excreted. There were 37% and 22% less variation between dung pats in Cr₂O₃ concentration when this was administered in shredded paper than when given in oil suspension.     

THE USE OF CATTLE WITH OESOPHAGEAL FISTULAE IN GRASSLAND EXPERIMENTS

Oesophageal-fistulated calves and cattle were used in various experiments during 1963 to 1966; results for 1966 are reported.
The method of inserting the fistula, the routine care of the animals and the methods of sampling through the fistula, are described.
Individual stall-feeding experiments in 1966 gave recovery values of 99.7% for herbage extruded through the fistulae, in relation to herbage eaten. The in vitro organic-matter digestibility of the grass offered and of the extrusa samples were identical. The digestibility of the grass fed in these experiments was high, but was comparable with that of herbage used in the grazing experiments reported. Grazing studies on grass/white clover pastures are reported. The digestibility was determined on all samples and they were separated into various plant fractions. Results obtained were compared with herbage samples cut at the same time. In most cases the digestibility and % N of the extrusa sample was higher than that of the corresponding herbage sample. The quantity of dead material present increased, and more was eaten, as the season advanced; it was of low digestibility. Results of intake studies with grazing animals are presented and the use of oesophagea-istulated cattle as an aid in such studies is discussed.     

Excavating sponges that are destructive to farmed pearl oysters in Western and Northern Australia

Species of the family Clionaidae (Porifera: Demospongiae) that excavate shell of the silver‐lip pearl oyster, Pinctada maxima, in north Western and Northern Australia are described. Two species belong to the genus Cliona and one species to the closely related genus Pione. Cliona orientalis has only recently been reported from Australia in living and dead coral on the Great Barrier Reef, and this is the first report of this species from north Western and Northern Australia. Cliona dissimilis is reported from Australia for the first time. Pione velans was first described from Shark Bay, Western Australia and this study extends its distribution from Albany, south Western Australia to Port Bremer in the Northern Territory. The most common species found was C. dissimilis. Pione velans and C. orientalis were also present, although the latter species was rare. Cliona dissimilis and P. velans were found to be sexually reproductive in some shells with specimens of C. dissimilis with oocytes in May and P. velans in September 1999. Egg development was synchronous, indicating that the sponges were oviparous and would broadcast gametes in a spawning event.     

Mutations in U4atac snRNA, a component of the minor spliceosome, in the developmental disorder MOPD I

Small nuclear RNAs (snRNAs) are essential factors in messenger RNA splicing. By means of homozygosity mapping and deep sequencing, we show that a gene encoding U4atac snRNA, a component of the minor U12-dependent spliceosome, is mutated in individuals with microcephalic osteodysplastic primordial dwarfism type I (MOPD I), a severe developmental disorder characterized by extreme intrauterine growth retardation and multiple organ abnormalities. Functional assays showed that mutations (30G>A, 51G>A, 55G>A, and 111G>A) associated with MOPD I cause defective U12-dependent splicing. Endogenous U12-dependent but not U2-dependent introns were found to be poorly spliced in MOPD I patient fibroblast cells. The introduction of wild-type U4atac snRNA into MOPD I cells enhanced U12-dependent splicing. These results illustrate the critical role of minor intron splicing in human development.     

STUDIES IN CALF MANAGEMENT

Calves born in late September to early October in 1956, 1957 and 1958 were reared at pasture. They were fed milk substitute, supplemented in some cases with concentrate feeding-stuffs.
The losses due to outdoor rearing were low, and calves made satisfactory gains in weight. By rearing at pasture, a considerable saving was effected in the weight of concentrates and hay consumed, while no marked response to the feeding of these supplements before weaning was found. Animals born in the autumn of 1957 and reared on this system reached 10 cwt. in less than 24 months.     

An empirical cohort model for management of Terra Firme forests in the Brazilian Amazon

A model to project forest growth in the Terra Firme forests of the eastern Amazon is described. It is based on 12–17 years measurements from experimental plots at Jarí and Tapajós. Forest stands are represented by cohorts of species group, diameter, and defect. There are 54 species groups, with a robust diameter increment function fitted to each, tables of mortality by crown and defect status, and recruit lists by disturbance level and locality. Stand level functions partition trees by crown status, and modify growth for stand density. Recruitment is a function of basal-area losses. Evaluation compares model performance with two experiments involving heavy felling in Tapajos State Forest. At one site, total bole volume growth of all species over 45 cm DBH was 2.56 m³ ha⁻¹ year⁻¹ over 17 years, whereas the model projected 3.13 m³ ha⁻¹ year⁻¹. At the other site, actual growth over 12 years was 0.39 m³ ha⁻¹ year⁻¹, with the model giving an identical result. Both felled and control plots are compared in the study and accurately simulated. Some weaknesses in the model are discussed.     

Adsorptive effects of di-tri-octahedral smectite on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies

OBJECTIVE: To determine the adsorptive capability of di-tri-octahedral smectite (DTOS) on Clostridium perfringens alpha, beta, and beta-2 exotoxins and equine colostral antibodies. SAMPLE POPULATION: 3 C perfringens exotoxins and 9 colostral samples. PROCEDURES: Alpha, beta, and beta-2 exotoxins were individually co-incubated with serial dilutions of DTOS or bismuth subsalicylate, and the amount of toxin remaining after incubation was determined via toxin-specific ELISAs. Colostral samples from healthy mares were individually co-incubated with serial dilutions of DTOS, and colostral IgG concentrations were determined via single radial immunodiffusion assay. RESULTS: Di-tri-octahedral smectite decreased the amount of each C perfringens exotoxin in co-incubated samples in a dose-dependent manner and was more effective than bismuth subsalicylate at reducing exotoxins in vitro. Decreases in the concentration of IgG were detected in samples of colostrum that were combined with DTOS at 1:4 through 1:16 dilutions, whereas no significant decrease was evident with DTOS at the 1:32 dilution. CONCLUSIONS AND CLINICAL RELEVANCE: Di-tri-octahedral smectite effectively adsorbed C perfringens exotoxins in vitro and had a dose-dependent effect on the availability of equine colostral antibodies. Results suggested that DTOS may be an appropriate adjunctive treatment in the management of neonatal clostridiosis in horses. In vivo studies are necessary to fully assess the clinical efficacy of DTOS treatment.     

STUDIES IN CALF MANAGEMENT

Spring-born calves were reared in 1956 and 1957 on (a) a multiple-suckling system, (b) bucket-feeding indoors and (c) bucket-feeding at pasture. Milk substitute was offered in similar quantities to (b) and (c); (a) and (b) received hay and concentrates; (c) received no concentrates but hay was offered in 1956. The average daily live-weight gains (lb.) in 1956 were 1·63, 1·30 and 1·45; in 1957 1·70, 1·50 and 1·55, respectively.
The importance of grass of good quality, free from parasitic infestation, is discussed.