The advantages of the use of vitamin E and selenium for the prevention and therapy of porcine stress syndrome

In a feeding trial lasting for months Halothane-positive fattening pigs were put on a diet containing different concentrations of vitamin E and selenium. After a Myostress application vitamin E and selenium were additionally injected in order to examine stress resistance (CK-test) and regenerative capacity (histological examination of Musculus longissimus dorsi) of skeletal muscle. The study revealed that neither high dietary levels of vitamin E/selenium nor a vitamin E/selenium treatment applied after stress are capable of preventing stress related myopathy nor can they influence its healing up favourably.     

Epidermal growth factor-like activity in mares' milk.

Epidermal growth factor (EGF)-like activity was measured in mares' colostrum and milk by radioreceptor assay. Milk samples were collected from 22 mares 1 or more times during early lactation. Samples of colostrum were taken after parturition and before the foal first suckled (presuckle), within 6 hours after the foal first suckled (postsuckle), and on days 1, 2, 4, and 8 of lactation. In the 5 mares from which milk samples were obtained at each sampling time, presuckle colostral mean EGF-like activity (17.8 ng/ml) was greatest (P less than 0.05). The mean values for EGF-like activity at all other sampling times were not significantly different from each other (postsuckle colostrum, 9.7 ng/ml; day 1, 9.6 ng/ml; day 2, 8.5 ng/ml; day 4, 8.0 ng/ml; day 8, 7.8 ng/ml).     

The effect of hypervitaminosis D3 on the bones of the hen. 1. Light microscopy studies

46 one day old chickens, divided into five groups, were fed an alternately composed vitamin D3 ration during 36 days. In the group depleted of vitamin D3 the typical rickets were found. When treated with a standard ration (2000 I.U. vit. D3/kg food) after 15 days depletion of vitamin D3 the chickens showed control-group parameters within 3 weeks. Using 60,000 I.U. vitamin D3 after a period of 15 days depletion evoked rather an abrupt beginning of healing tendency accompanied by signs of intoxication which were enhanced in those chickens treated with 120,000 I.U. vitamin D3: increase in number of osteoblasts, calcification of osteoblastic mitochondrial membranes and endothelial cell membranes, as well as degeneration of osteoblasts together with a strong reaction of eosinophils. There did not occur necrosis of osteocytes or of bone structure. Erythrocytes, being embedded in ground substance without endothelial surrounding, was a common finding in every group.     

Genotypic Variability in Biomass Protein and Nucleic Acids in Four Linum Species to Sodicity Stress

Differences in gas exchange parameters i.e. carbon exchange rate (CER), transpiration (Tr), water vapor conductance (g) were investigated, under a controlled environment, in a semi-dwarf (SD) Triticum aestivum line, its tall (TL) near isoline and the Payne (PA) variety characterized by small leaves. The plants were maintained in: (a) optimal substrate moisture condition (CTR); (b) salinized by watering with a 0.12 m NaCl solution (SLT); (c) water stressed by withholding waterings during a period of six days (STR); (d) stressed and salinized during the same period and with the same saline solution (S + S). CER and Tr were negatively affected by the stresses; SLT and S + S treatments had a higher WUE compared to their respective controls CTR and STR.
SD line had a better performance in terms of CER and WUE, particularly when water and salt stresses interacted. Leaf anatomy and assimilation/internal CO₂ concentration curves contributed to explaining the SD performance.     

Staining pattern of the brush border and detection of cytoplasmic granules in the uriniferous tubules of female DBA/2Cr mouse kidney: comparison among various fixations and stains.

The proximal straight tubules of the female mouse kidney exhibit heavy periodic acid Schiff (PAS) staining in their brush borders and numerous cytoplasmic granules. In the present study, the female DBA/2Cr mouse kidney was examined, using various fixatives (formalin, PFA, PLP, Zamboni's, Bouin, or Carnoy solution) and various staining methods (HE, PAS, alcian blue, periodic acid methenamine-silver (PAM), toluidine blue, azan, or Congo red). Under azan and PAM, the staining pattern of the brush border was similar to that of PAS, and few effects of the fixative were observed. Cytoplasmic granules were clearly detected with PAM as well as PAS. However, these granules were not detected with Carnoy solution. Furthermore, distribution of granules differed between PAS and PAM.     

竹材弦面纵向切削的研究

研究了竹材弦面纵向切削的3种切削方式(单劈刀切削、单刨刀切削及并列式双刨刀切削)及单刨刀切削时的切削阻力,结果表明:对竹材采用劈或刨的切削方式,均可进行弦面纵向切削并能获得质量较好的竹蔑。竹材的节间、节部、切削厚度、含水率及切削速度等因素对切削阻力均有不同程度的影响。节部切削阻力明显高于节间切削阻力;切削阻力随切削厚度的增大而增大,两者成线性关系;含水率增大时,节间切削阻力减小,而节部切削阻力变化不大;切削速度增大时,节间切削阻力基本上是下降的,而节部切削阻力是增大的。     

Antibody response to crustacean ectoparasites in rainbow trout, Oncorhynchus mykiss (Walbaum), immunized with Argulus foliaceus L. antigen extract

Abstract. A humoral antibody response was demonstrated by ELISA in rainbow trout immunized intraperitoneally with extracts from the branchiuran ectoparasite Argulus foliaceus. A similar immunization protocol produced a higher titre response in a rabbit. Both trout and rabbit identified several antigenic components on immunoblots. ELISA and immunoblotting indicated that rainbow trout and rabbit anti-A. foliaceus sera identified components from the parasitic copepods Lepeophtheirus salmonis and Caligus elongatus.     

无核葡萄胚发育及早期离体培养的研究:Ⅲ.培养方式对离体胚发育的影响

对5个无核葡萄品种的试验表明,不同培养方式对离体胚发育的影响是很明显的,其中4个品种胚发育率液体培养高于固体培养,‘京早晶’在液体培养中胚发育率高达73.7%,是目前国内外胚发育率最高的报道;液体培养比固体培养胚的发育程度高,发育整齐,无畸形胚,易成苗.     

Use of monoclonal antibodies against human HLA II antigens for the detection of bovine B lymphocytes and macrophages]

The crossreactivity of mouse monoclonal antibodies (MoAbs) (Tab. I) prepared against human HLA-DR and HLA-DP antigens was studied in various bovine cells: lymphocytes from lymph nodes and peripheral blood, adherent (B) and nonadherent (T) lymphocytes, monocytes, granulocytes and platelets. In the immunofluorescence test, MoAbs Bra13, Bra14, Bra20, Bra22, Bra30, Bra70, HL-38 reacted with bovine B lymphocytes and monocytes, but not with other tested cells (Tab. III, IV). These antibodies, except Bra22, were positive with B lymphocytes in the complement dependent cytotoxic test (Tab. II). The similarity of the bovine antigens and HLA-DR antigens determined by used MoAbs was also proved by immunoblotting. Monoclonal antibodies Bra38 and BraFB6 did not react with the bovine cells and separated antigens. The epitope (HLA-DR) recognized by the antibody Bra38 is probably absent in cattle. The presence of HLA-DP analogue determined by the antibody BraFB6 has not been confirmed. The crossreactive MoAbs could be used for the detection of B lymphocytes and macrophages in veterinary immunology.