Genetic analysis of selected almond genotypes and cultivars grown in Turkey using peroxidase-gene-based markers

Peroxidase plays important roles in many stress-related interactions and catalyzes important reactions in various physiological processes. Since peroxidase played critical roles in the evolution of almond(Prunus dulcis Miller(D.A Webb) syn P. amygdalus Batsch), peroxidase-gene-based analyses may increase the understanding of evolution of this species. Peroxidase gene polymorphism(POGP) markers were used to determine genetic diversity and relationships among 69 Turkish genotypes/cultivars and 27 foreign almond cultivars by using unweighted pair group method with arithmetic mean(UPGMA) analysis. This study is the first evaluation of the use of POGP markers for diversity analysis in almond.Totally, 83 fragments were obtained from eight peroxidase primer pairs, and polymorphism was identified as 94 %.Similarity level among the genotypes ranged between 0.63 and 0.93, and all materials were distinguished. In general,Turkish and foreign genotypes were mixed in clusters since they share a common genetic background and gene migration among the sites. Clusters were not based on geographic regions except for some minor groupings. This study indicated that peroxidase gene markers can be reliably used to determine genetic relationships in almonds.     

Variation in juvenile traits of natural black poplar (Populus nigra L.) clones in Turkey

Natural black poplar (Populus nigra L.) clones sampled from river courses were tested in the arid southeast region of Turkey, using a randomized complete block field design with four replications. Clones were laid out in row plots of eight ramets each. Height and apical dominance were assessed at age one year; diameter, survival, bole straightness and branchiness were measured at age two years. Clones differed significantly in survival, growth and quality traits. The results showed that promising clones exist in natural populations. Two commercial clones out of four did not grow as fast as the top new selection clones. For bole straightness, three commercial clones had significantly lower grading scores than the top best 14 clones. Principal components analysis indicated that growth, apical dominance and branching are the most important traits distinguishing black poplar clones. Diameter had a moderate correlation (0.34) with bole straightness. Relationships between geographic variables (elevation, latitude and longitude) with growth and stem quality traits were weak. Considerable genetic variation was observed among clones for all the traits. Genetic differences among the clones accounted for 27% (survival, bole straightness) to 39% (height) of the total variance. Broad-sense individual heritability ranged from 0.27 (survival) to 0.37 (apical dominance). Clonal mean heritabilities were higher than individual heritabilities and ranged from 0.60 (survival) to 0.82 (diameter), implying considerable gain could be realized via selective improvement methods.     

Determination of Heat Requirements and Effective Heat Summations of Some Pomegranate Cultivars Grown in Southern Anatolia

Temperature (high and low) is one of the most limiting environmental factors for growth and production of fruits in different parts of the world. For temperate-zone fruit species, a certain total heat requirements are needed in order to obtain ripe fruits. The aim of this study was to determine heat requirements of three commercial pomegranate cultivars (‘Suruc’, ‘Katirbasi’ and ‘Hicaznar’) grown in Southern Anatolia region. The cultivars showed a narrower range of heat requirements for flowering (growing degree hours, GDH), i.e. ‘Suruc’ (25,000), ‘Katirbasi’ (25,270), ‘Hicaznar’ (28,000) and a wide range of heat requirements for fruit ripening, i.e. ‘Suruc’ (73,670), ‘Katirbasi’ (74,105) and ‘Hicaznar’ (88,052) GDH. Under the Sanliurfa province conditions, two years average of effective heat summation requirement from bud swelling to 50 % flowering stage for ‘Suruc’, ‘Katirbasi’ and ‘Hicaznar’ cultivars are calculated as 643, 655 and 718 growing degree days (GDD) and from bud swelling to harvest are 2734, 2802 and 3289 GDD. It can be concluded that the effective heat summation of Sanliurfa province is enough for commercial growing for all pomegranate cultivars.     

Biodiversity of figs (Ficus carica l.) in Coruh valley of Turkey

The Coruh valley lies within the Caucasus ecological zone, which is considered by the World Wild Fund for Nature and by Conservation International as a one of the biodiversity hotspot in the world. The valley is also recognized by Turkish conservation organizations as an important plant area, an important bird area, a key biodiversity area and has been nominated as a high priority area for protection. This valley is rich in plants and contains 104 nationally threatened plant species of which 67 are endemic to Turkey. Fig (Ficus carica L.) is one of the most important wild edible fruit tree along with pomegranate and olive in the valley. Figs have been used for fresh consumption for centuries and also an important element of natural landscape of the valley. The valley abundantly has black, purple and yellow-green fruited fig trees. In this study we determined some important tree, leaf and fruit morphological characteristics of 50 selected wild grown fig accessions naturally found in the valley. The results showed that the investigated morphological traits of fig accessions displayed significant differences each other. Principal coordinate analysis showed that diversity among the accessions was high and the accessions had black and yellow-green colored fruits were the most diverse groups.     

Formation of Resistant Starch by a Twin-Screw Extruder

The objective of this study was to evaluate the potential to increase the level of resistant starch (RS) in extruded products by optimizing extruder conditions. Three experiments were conducted as randomized complete block designs with two replicates. In the first experiment, corn starch, wheat starch, and potato starch were added at a level of 30% (w/w) to degerminated yellow corn meal to investigate the influence of starch type. In the second experiment, citric acid (CA) monohydrate was added to corn meal at levels of 0, 2.5, 5, and 7.5% (w/w). The third experiment was a full-factorial arrangement to evaluate the effect of high-amylose corn starch (HACS) level (0, 15, 30%, w/w) and CA level (0, 5, 7.5%, w/w) at two screw speeds (200 and 300 rpm). In the first experiment, the means for RS plus dietary fiber for the different starch formulations ranged from 1.27 to 2.28%. In experiment 2, adding CA increased RS plus dietary fiber content to a maximum of 5.23% at 7.5% CA. In the third experiment, the means for RS plus dietary fiber ranged from a low of 1.75% for 100% corn meal at 300 rpm to 14.38% for 7.5% CA and 30% HACS at 200 rpm. The results indicated a highly significant positive relationship between CA and RS formation and the same for amylose content. The RS formation had a negative relationship with screw speed, but the influence of screw speed was small when compared with that of CA and HACS.     

The effects of different temperatures and relative humidity on the development,mortality and nymphal predation of <Emphasis Type="BoldItalic">Anthocoris minki</Emphasis>

Mass rearing techniques are a necessity in developing a suitable and economic biological control method. Anthocoris minki Dohrn (Heteroptera: Anthocoridae), which is successfully reared for use in biological control, is a promising indigenous Anthocoris species for biological control of Agonoscena pistaciae Burckhardt and Lauterer (Homoptera: Psyllidae) in pistachio orchards. Development time of nymphal instars, mortality percentage and prey consumption of A. minki fed on Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) eggs were studied at three constant temperatures (20, 25 and 30 ± 1°C) and two relative humidity levels (40 and 65 ± 5%) under laboratory conditions. Temperature and r.h. significantly affected development time and nymphal prey consumption of A. minki. Duration of incubation and total nymphal development time decreased significantly with each increase in temperature. Total nymphal development time of A. minki at 40% and 65% r.h. was 18.6 and 18.6 days at 20°C; 13.7 and 14.6 days at 25°C; and 10.8 and 11.8 days at 30°C, respectively. The shortest nymphal development time and highest nymphal mortality percentage were recorded at 30°C and 40% r.h. The lowest nymphal mortality was 30.1% and 32.1% at 40% and 65% r.h., respectively, at 25°C; the highest nymphal mortality was 51.5% and 52.1% at 20° and 30°C, respectively, and 40% r.h. The highest average number of E. kuehniella eggs consumed was 123.1 at 20°C and 65% r.h., and the lowest was 86.4 at 30°C and 40% r.h. during nymphal development time of A. minki.     

Mating System and Pollen Contamination in a Pinus brutia Seed Orchard

Mating system parameters and pollen contamination were estimated in an 11-year-old Pinus brutia Ten. clonal seed orchard, located in Antalya, Turkey, with the aid of isozymes. Isozyme analysis was performed on both maternal (megagametophyte) and embryo tissues of seeds collected from the seed orchard and the surrounding natural stand. Fourteen loci encoding nine enzyme systems were assayed. Based on a multilocus estimator, the proportion of viable seeds originating from outcrossing (tˆ_m) in the seed orchard was found to be 0.947; the remainder (0.053) was due to selfing. We estimated that 85.7% of the orchard seed resulted from pollen contamination by trees in surrounding stands. This may be related to the young age and subsequent low pollen production of the orchard. Only 9% [i.e., 1.0−0.857 (contamination)−0.053 (selfing) = 0.090] of the orchard offspring was due to cross-pollination among clones within the orchard. Due to the high level of pollen contamination, it is estimated that genetic gain in this seed crop is less than 57% of that expected if all offspring resulted from random mating among orchard clones (i.e., no contamination).     

Critical period for weed control in sesame production

Planning effective weed control in cropping systems requires exact appraisal of the weed intensity and duration of their competition with the crops. This 2‐year study was carried out in order to determine the critical weed control period in sesame fields. Related and relative crop yields were monitored and analyzed using a four‐parametric log‐logistic model. We recorded data from weed‐free plots and compared these with data from different periods of weed interference. In both the study years, the longer period of weed interference decreased the relative yield of sesame, whereas the yield was increased with increasing duration of the weed‐free period. A 51–78.7% decline in sesame yield was noted if the weeds were allowed to compete with the crop from planting to harvest. In the first year, the duration of the critical period for weed control (CPWC) was 177–820 growing degree days (GDD), which corresponded to 14–64 days after crop emergence (DAE), and between 170 and 837 GDD (13–64 DAE) in the second year; this was based on a 5% acceptable yield loss. The results of this study clearly elaborated that maintaining weed‐free conditions is compulsory from as early as the second week after the emergence of sesame plants, and this should be maintained at least until the ninth week to avoid sesame yield losses by more than 5%. These findings show that growers can benefit from CPWC to improve weed control in sesame production, including the efficacy of a weed control program and its cost.     

Genetic variation in <Emphasis Type="Italic">Pinus taeda</Emphasis> wood properties predicted using non-destructive techniques

• Background   

Tree breeders have been reluctant to include wood traits in tree improvement programs owing to logistic difficulties and the cost associated with the assessing the traits.