Histologic characteristics and local cellular immunity of the gland of the third eyelid after topical ophthalmic administration of 2% cyclosporine for treatment of dogs with keratoconjunctivitis sicca

OBJECTIVE: To evaluate the efficacy of topical administration of a 2% solution of cyclosporine (CsA) for treatment of dogs with keratoconjunctivitis sicca (KCS) and to correlate results with histopathologic characteristics and local cellular immunity of the gland of the third eyelid. ANIMALS: 24 dogs with bilateral KCS. PROCEDURE: Lacrimal secretion was measured, using Schirmer tear test (STT) strips. Leukocyte and T-lymphocyte subsets were determined in blood samples. Histopathologic changes as well as CD4+, CD8+, and alpha-naphthyl-acetate esterase-positive (ANAE+) lymphocytes were evaluated. RESULTS: Clinical signs resolved at the end of 1 month in conjunction with significantly increased STT values, compared with baseline values. Fifteen and 30 days after discontinuation of CsA treatment, a decrease was observed in STT values in both eyes; however, only values for the right eye were significantly different. There was a significant decrease in the number of lymphocytes and ANAE+ lymphocytes 15 and 30 days after discontinuation of CsA treatment, compared with baseline values. Differences were not observed in number of CD4+ lymphocytes among treatment groups. However, there was a significant decrease in number of CD8+ lymphocytes with reversal of the CD4+:CD8+ in both eyes after CsA treatment for 30 days, compared with the control group. Increased secretory activity and decreased lymphocyte infiltration were characteristic histopathologic findings. CONCLUSIONS AND CLINICAL RELEVANCE: Topical administration of a 2% solution of CsA was effective for the treatment of dogs with KCS. Strict follow-up monitoring is required after the cessation of treatment because of the possibility of recurrence of KCS.     

Zur Sch?dlingsvertilgung einiger Singvogelarten in einem Schwarzkiefern/Eichen-Mischbestand im Beynamer Wald bei Ankara

Analysis of nestling food in four species of Parus and one species of Ficedula using the 'Halsringmethode' showed a high quota of adults and caterpillars of the important forest pests: Tortrix viridana, Euproctis chrysorrhoea, Diprion pini, and Lymantria dispar. These four major pest species amounted in the nestling food of Parus major to 50%, P. coeruleus to 52.6%, P. ater to 40.9%, and Ficedula hypoleuca to 30.2%.     

Influence of Different Soilless Substrates to Morpho-physiological Characteristics and Yield Relations in Strawberries

Some of the advantages of soilless production systems over conventional systems are higher yield, better pest control management and more efficient labor use. Among berries, strawberry responds quite well to soilless production systems. Major yield-affecting factors for strawberries in soilless systems may be listed as; growing medium, source and types of plantlets, density of plants and efficiency of fertigation system. The present research was conducted with strawberry plants (Fragaria x ananassa ‘Camarosa’) grown in four different media; (1) Cocopeat (C), (2) Peat (P) and (3 and 4) 1:1 mixtures with Pumice (Pu), to evaluate the effects of soilless media on marketable yield and root-shoot morpho-physiological parameters. Correlation measurements were made to assess if there is any interaction between yield and any tested morpho-physiological feature. The experiment was conducted in greenhouse, using frigo plantlets (11.6 plantlet/m²) planted in July and through 1 June of following year. Results showed that growth medium had an effect on yield, numbers of developed leaves, shoot-root dry mass, number and length of roots. Yield was correlated with these morphological features. Growth medium also had an influence on plant nutrient accumulations i.?e., shoot accumulation of nitrogen (N) and potassium (K) and root accumulation of phosphorus (P) and all the micro-nutrients that with exception of boron (B). There were some interactions between yield and plant nutrient content of tissues determined by correlation analyses. The most striking were negative correlations with yield and shoot N–K amounts and positive correlation with yield and root P amount. Some positive correlations were also found between yield and copper (Cu), manganese (Mn) and zinc (Zn) contents of both shoot and root tissues. Boron (B) contents was correlated only with the root tissues. The medium which had the best overall performance for soilless strawberry production was C?+?Pu and should be recommended to be used with frigo plantlets of ‘Camarosa’.     

Toscana virus (TOSV) exposure is confirmed in blood donors from Central, North and South/Southeast Anatolia, Turkey

Toscana virus (TOSV), a sandfly fever virus serotype of medical and public health importance, is a major pathogen involved in aseptic meningtis occurring in Mediterranean countries and poses a threat to the residents as well as travellers. Limited data on TOSV activity are present from Turkey despite being located in the endemic zone. We aimed to identify TOSV exposure in 1115 healthy blood donors at the Hacettepe University Hospital Blood Bank in Ankara, Turkey, using commercial indirect fluorescence assays (IFAs) and virus neutralization test (VNT) for antibody detection and specificity confirmation. A total of 199 samples (17.8%) were positive for anti-TOSV that include IgG reactivity in 10.4%, IgM reactivity in 8.2% and IgM + IgG reactivity in 0.7% of the sera. Anti-TOSV specificity could be confirmed via VNT in 56% of the IgG- and 43.6% of the IgM-positive sera, making up a total of 58 samples (5.2%). Risk factors associated with TOSV IgG reactivity were male gender, residing in rural areas, frequent sighting of mosquitoes/sandflies and working outdoors. TOSV-specific antibody prevalence increased significantly with age. Evidence of exposure to other sandfly fever viruses was noted. These data reveal that mild or asymptomatic infections with TOSV are frequent in central and northern Anatolia. TOSV exposure has also been identified in residents of 9 provinces in southern/southeastern Anatolia for the first time.     

Zur Sch?dlingsvertilgung einiger Singvogelarten in einem Schwarzkiefern/Eichen-Mischbestand im Beynamer Wald bei Ankara

Analysis of nestling food in four species of Parus and one species of Ficedula using the 'Halsringmethode' showed a high quota of adults and caterpillars of the important forest pests: Tortrix viridana, Euproctis chrysorrhoea, Diprion pini, and Lymantria dispar. These four major pest species amounted in the nestling food of Parus major to 50%, P. coeruleus to 52.6%, P. ater to 40.9%, and Ficedula hypoleuca to 30.2%.     

Zur Schädlingsvertilgung einiger Singvogelarten in einem Schwarzkiefern/Eichen-Mischbestand im Beynamer Wald bei Ankara

Analysis of nestling food in four species of Parus and one species of Ficedula using the 'Halsringmethode' showed a high quota of adults and caterpillars of the important forest pests: Tortrix viridana, Euproctis chrysorrhoea, Diprion pini, and Lymantria dispar. These four major pest species amounted in the nestling food of Parus major to 50?%, P. coeruleus to 52.6?%, P. ater to 40.9?%, and Ficedula hypoleuca to 30.2?%.     

Effect of altitude on the composition of suberin monomers in the outer bark of Scots pine (Pinus sylvestris L.)

The changes in the amount and composition of suberin monomers of Scots pine outer bark, which is grown natively in Turkey, was investigated as a function of growing altitude. Samples were taken from every 100 m to 1300 m which was the highest point of the sampling area. While the total amount of suberin monomers is varied within the range 17.56-47.20 mg/g, there was no correlation between total amount and altitude. Total amount of alkanols of suberin monomers decreased when the altitude increased. Alcohol 24:0 was seen as the dominant constituent and its amount decreased from 100 m to 1300 m. There was not a clear change in the total amount of alkanoic, dioic and hydroxy acids. Acid 1,18-hydroxy-18:1 and acid 1,18-dioic-18:1 were determined as the main compounds in all samples.     

Prof. Dr. Abdulgafur Acatay in memoriam

Ohne Zusammenfassung