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Simone Taddei Chiara Robbi Camilla Cesena Ilenia Rossi Emiliana Schiano Norma Arrigoni Gaddo Vicenzoni Sandro Cavirani 《Journal of veterinary diagnostic investigation》2004,16(6):503-508
Three commercially available assays, designed to specifically detect the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in fecal samples by IS900-PCR, were compared with a conventional culture method. Fecal samples from 100 dairy cows were tested. Fifty-four (67.5%) of 80 culture-positive samples were positive for an assay that detects MAP DNA by dot spot hybridization of polymerase chain reaction products (kit A), 48 (60%) were positive by an assay using ethidium bromide staining for agar gel visualization of amplification products (kit B), and 49 (61.3%) were positive by an assay in which amplified products are detected by a colorimetric detection system (kit C). Relative sensitivity of all tests increased in proportion to the presence of MAP in fecal samples. Specificity was 100% based on results from 20 culture-negative samples from an MAP-free herd. 相似文献
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Ilenia Siciliano Giovanna Gilardi Giuseppe Ortu Ulrich Gisi Maria Lodovica Gullino Angelo Garibaldi 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(2):401-413
Alternaria species are common pathogens of fruit and vegetables able to produce secondary metabolites potentially affecting human health. Twenty-nine isolates obtained from cabbage, cauliflower, wild and cultivated rocket were characterized and identified based on sporulation pattern and virulence; the phylogenetic analysis was based on the β-tubulin gene. Isolates were identified as A. alternata, A. tenuissima, A. arborescens, A. brassicicola and A. japonica. Pathogenicity was evaluated on plants under greenhouse conditions. Two isolates showed low level of virulence on cultivated rocket while the other isolates showed medium or high level of virulence. Isolates were also characterized for their mycotoxin production on a modified Czapek-Dox medium. Production of the five Alternaria toxins, tenuazonic acid, alternariol, alternariol monomethyl ether, altenuene and tentoxin were evaluated. Under these conditions, about 80% of the isolates showed the ability to produce at least one mycotoxin. 相似文献
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Pintore Ilenia Gilardi Giovanna Garibaldi Angelo Gullino Maria Lodovica 《植物病害和植物保护杂志》2018,125(3):297-309
Journal of Plant Diseases and Protection - The efficacy of different fungicides, belonging to different chemical groups, was in vitro and in vivo tested in glasshouse trials against Allophoma... 相似文献
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