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1.
Martins ML Vieira FN Jerônimo GT Mouriño JL Dotta G Speck GM Bezerra AJ Pedrotti FS Buglione-Neto CC Pereira G 《Fish physiology and biochemistry》2009,35(1):219-222
This study evaluated the total and differential leukocyte counting and the phagocytic activity in Nile tilapia Oreochromis niloticus experimentally injected with Enterococcus sp. in the swim bladder. Fish were distributed in four treatments in triplicates of non-injected fish, fish injected with
1 ml of sterile saline solution 0.65%, and fish injected with 1 × 103 and 1 × 106 colony-forming units (CFU) of Enterococcus diluted in 1 ml sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected for
white blood cell (WBC) counts, differential counting of WBC, and phagocytic activity of blood leukocytes. The increased numbers
of WBC and lymphocytes were followed by decreased number of monocyte after infection. The percentages of phagocytic activities
in the blood were 55.3 and 55.9%, respectively, in tilapia injected with 1 × 103 and 1 × 106 CFU/ml. 相似文献
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Synthesis,theoretical studies,and effect on the photosynthetic electron transport of trifluoromethyl arylamides 下载免费PDF全文
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Effects of temperature on growth,survival and physiological parameters in juveniles of Lophiosilurus alexandri,a carnivorous neotropical catfish 下载免费PDF全文
Daniel Pereira da Costa Fabíola de Oliveira Paes Leme Rodrigo Takata Deliane Cristina Costa Walisson de Souza e Silva Reinaldo Melillo Filho Gabriela Miccoli Alves Ronald Kennedy Luz 《Aquaculture Research》2016,47(6):1706-1715
This study aimed to analyse the effects of different water temperatures on the growth, survival and blood physiological parameters of Lophiosilurus alexandri. Juveniles measuring 12.6 ± 0.5 cm and weighing 30.6 ± 3.6 g were subjected to four water temperature regimes: 23, 26, 29 and 32°C with four replicates. The animals were fed twice daily with formulated diet. The experiment lasted for 35 days. Survival was 100% at all water temperatures, and the best temperature for growth was estimated to be 27.8°C using a quadratic regression model. The estimated temperatures for the best feed conversion and highest feed consumption rates were 26.2 and 28.8°C respectively. The fat visceral‐somatic index was highest at 32°C. The highest haemoglobin and haematocrit values were 29.4 and 32.1°C respectively. For plasma protein and cholesterol, the lowest values estimated were 23.9 and 24.1°C respectively. Glucose and liver nitrogen content increased with rising temperature. Higher values of total ammonia in the water were observed at higher temperatures. No effects of temperature were seen on the amounts of muscle dry matter, nitrogen, energy content and triglycerides or on the hepatosomatic index. Water temperature has an important role in the developmental and blood physiological parameters of juvenile L. alexandri, and the quadratic regression model showed that the appropriate temperature for growth is between 27 and 28°C. 相似文献
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Veras HN Rodrigues FF Colares AV Menezes IR Coutinho HD Botelho MA Costa JG 《Fitoterapia》2012,83(3):508-512
Lippia sidoides Cham. (Verbenaceae) is used in the folk medicine as topical antiseptic in skin and mucous membranes and its therapeutic effect is attributed to the thymol presence. The objective of this work was to verify the chemical composition and antibiotic modifying activity of the essential oil extracted from the leaves of L. sidoides and its major component thymol. The essential oil was obtained by hydrodistillation and analyzed by GC/MS. The synergistic activity was evaluated using gaseous contact method. The essential oil was obtained (yield of 1.06%) and the GC/MS analysis identified the main constituents: thymol (84.9%) and p-cymene (5.33%). The antibiotic modifying activity was verified using the minimal inhibitory dose method and gaseous contact. It verified the interference of essential oil and thymol against all tested aminoglycosides. There were no statistical differences between the activity of the essential oil and thymol against Pseudomonas aeruginosa, indicating this to be the responsible composition for such activity. However, the oil was shown more effective when compared to the thymol against Staphylococcus aureus. The essential oil of L. sidoides and its major component thymol influence the activity of aminoglycosides and may be used as adjuvant in antibiotic therapy against respiratory tract bacterial pathogens. 相似文献
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Queiroz TM Machado NT Furtado FF Oliveira-Filho AA Alustau MC Figueiredo CS Miranda GE Barbosa-Filho JM Braga VA Medeiros IA 《Marine drugs》2011,9(10):2075-2088
This study aimed to investigate the cardiovascular effects elicited by Dictyota pulchella, a brown alga, using in vivo and in vitro approaches. In normotensive conscious rats, CH(2)Cl(2)/MeOH Extract (CME, 5, 10, 20 and 40 mg/kg) from Dictyota pulchella produced dose-dependent hypotension (-4 ± 1; -8 ± 2; -53 ± 8 and -63 ± 3 mmHg) and bradycardia (-8 ± 6; -17 ± 11; -257 ± 36 and -285 ± 27 b.p.m.). In addition, CME and Hexane/EtOAc Phase (HEP) (0.01-300 μg/mL) from Dictyota pulchella induced a concentration-dependent relaxation in phenylephrine (Phe, 1 μM)-pre-contracted mesenteric artery rings. The vasorelaxant effect was not modified by the removal of the vascular endothelium or pre-incubation with KCl (20 mM), tetraethylammonium (TEA, 3 mM) or tromboxane A(2) agonist U-46619 (100 nM). Furthermore, CME and HEP reversed CaCl(2)-induced vascular contractions. These results suggest that both CME and HEP act on the voltage-operated calcium channel in order to produce vasorelaxation. In addition, CME induced vasodilatation after the vessels have been pre-contracted with L-type Ca(2+) channel agonist (Bay K 8644, 200 nM). Taken together, our data show that CME induces hypotension and bradycardia in vivo and that both CME and HEP induce endothelium-independent vasodilatation in vitro that seems to involve the inhibition of the Ca(2+) influx through blockade of voltage-operated calcium channels. 相似文献
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Pereira FM Pereira-Filho ER Bueno MI 《Journal of agricultural and food chemistry》2006,54(16):5723-5730
In this study an analytical methodology for food analyses combining X-ray spectroscopy (XRS) with partial least-squares (PLS) data treatment was developed. Fifteen tea samples were purchased at a local market, and XRS spectra were obtained without sample pretreatment. For comparison of the metal concentrations, the samples were also mineralized, and six elements were determined using flame atomic absorption spectrometry (Ca, Fe, Mg, and Mn), flame atomic emission spectrometry (K), and thermospray flame furnace atomic absorption spectrometry (Zn). The spectral information obtained from XRS and the metal concentrations found using the alternative techniques were employed to generate six PLS models. The Ca and Mn models required four latent variables (LV), Fe, five LV, K, two LV, and Mg and Zn, three LV. The limits of quantification for these models were 614, 134, 761, 140, 85, and 1 mg kg(-1) for Ca, Fe, K, Mg, Mn, and Zn, respectively. 相似文献
9.
de Melo Ocarino N Fukushima FB de Matos Gomes A Bueno DF de Oliveira TS Serakides R 《Journal of Feline Medicine and Surgery》2006,8(5):345-348
This report describes the first case of idiopathic hypertrophic osteopathy (HO) in a cat. No causes for the bone pathology were found following evaluation of the physical and laboratory examinations (complete blood count, albumin, creatinine, urea, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase and gamma-glutamyltransferase and urinalysis), and after histopathological evaluation of organs at necropsy. Based on the radiographic, clinical and anatomopathological findings, idiopathic HO was diagnosed. 相似文献
10.
Camila Da Costa De Quadros Karina Oliveira Lima Caio Hendrix Luz Bueno Fabíola Helena dos Santos Fogaça Meritaine Da Rocha Carlos Prentice 《Journal Of Aquatic Food Product Technology》2019,28(6):677-688
The objective of this study was to obtain protein hydrolysate from the mechanically separated meat of blackfin pacu to evaluate the influence by ultrafiltration in the antioxidant and antimicrobial activities of the peptide fractions obtained and to apply in ground beef to evaluate the lipid stability. The enzymatic hydrolysis was performed using the enzyme Protamex (pH 7.0, 60°C) for 240 min. The protein hydrolysate was fractionated by ultrafiltration. Then, the antioxidant capacity of the protein hydrolysate and the peptide fractions were evaluated in vitro by the methods of 2,2’-azinobis (3-ethylbenzothiazoline sulfonic acid) radical capture, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay, reducing power, and hydroxyl radical scavenging activity. The antimicrobial activity of the samples was evaluated by disc-diffusion against Staphylococcus aureus and Escherichia coli. After evaluation, the peptide fractions did not present higher bioactivities than that shown for the hydrolysate. The protein hydrolysate was applied to ground beef, where the substances reactive to thiobarbituric acid and color were evaluated during 7 days of storage at 4°C. Lipid oxidation was reduced up to 60.9% and there was no modification of the natural coloration. Thus, the protein hydrolysate can be used as an alternative source of antioxidant for the preservation of refrigerated meats. 相似文献