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排序方式: 共有77条查询结果,搜索用时 15 毫秒
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Microinjection of a conserved peptide sequence of p34cdc2 induces a Ca2+ transient in oocytes 总被引:3,自引:0,他引:3
A Picard J C Cavadore P Lory J C Bernengo C Ojeda M Dorée 《Science (New York, N.Y.)》1990,247(4940):327-329
The product of the yeast cell cycle control gene cdc2, and its homologs in higher eukaryotes (p34cdc2), all contain a perfectly conserved sequence of 16 amino acids that has not been found in any other protein sequence. Microinjection of this peptide triggers a specific increase in the concentration of intracellular free Ca2+ that originates from intracellular stores in both starfish and Xenopus oocytes. Thus, p34cdc2 might interact through its conserved peptide domain with some component of the Ca2(+)-regulatory system. 相似文献
3.
Colbourne JK Pfrender ME Gilbert D Thomas WK Tucker A Oakley TH Tokishita S Aerts A Arnold GJ Basu MK Bauer DJ Cáceres CE Carmel L Casola C Choi JH Detter JC Dong Q Dusheyko S Eads BD Fröhlich T Geiler-Samerotte KA Gerlach D Hatcher P Jogdeo S Krijgsveld J Kriventseva EV Kültz D Laforsch C Lindquist E Lopez J Manak JR Muller J Pangilinan J Patwardhan RP Pitluck S Pritham EJ Rechtsteiner A Rho M Rogozin IB Sakarya O Salamov A Schaack S Shapiro H Shiga Y Skalitzky C Smith Z Souvorov A Sung W 《Science (New York, N.Y.)》2011,331(6017):555-561
We describe the draft genome of the microcrustacean Daphnia pulex, which is only 200 megabases and contains at least 30,907 genes. The high gene count is a consequence of an elevated rate of gene duplication resulting in tandem gene clusters. More than a third of Daphnia's genes have no detectable homologs in any other available proteome, and the most amplified gene families are specific to the Daphnia lineage. The coexpansion of gene families interacting within metabolic pathways suggests that the maintenance of duplicated genes is not random, and the analysis of gene expression under different environmental conditions reveals that numerous paralogs acquire divergent expression patterns soon after duplication. Daphnia-specific genes, including many additional loci within sequenced regions that are otherwise devoid of annotations, are the most responsive genes to ecological challenges. 相似文献
4.
Decreased respiratory burst activity in neonatal bovine neutrophils stimulated by protein kinase C agonists. 总被引:1,自引:0,他引:1
Newborn calves have a high susceptibility to bacterial infections, which may be related to the impaired neutrophil defense functions in newborns. The oxygen-dependent production of the free radical superoxide anion (O2-) represents an important part of the leukocyte respiratory burst central to neutrophil-directed defenses against bacterial infection. Because protein kinase C (PKC) activation is considered to be an important step in the signal transduction pathway for the O2- generating system, we compared O2- production by newborn and adult bovine neutrophils stimulated with 3 different PKC agonists. When the phorbol ester phorbol 12-myristate 13-acetate (PMA) was used, PKC-dependent O2- generation from newborn neutrophils was significantly reduced (P less than 0.01) for all concentrations of PMA tested (10, 100, and 500 ng/ml). In addition, newborn neutrophils had a significantly (P less than 0.01) reduced lag time for O2- generation. Similar significantly (P less than 0.01) reduced O2- generation from newborn neutrophils was observed with an additional phorbol ester (phorbol 12,13-dibutyrate); lag times were not calculated for phorbol 12,13-dibutyrate. When O2- generation was stimulated with a synthetic diacylglycerol analogue (1,2-dioctanoyl-sn-glycerol), less O2- was generated from both adult and newborn neutrophils than was obtained with the phorbol esters, and newborn neutrophils produced significantly (P less than 0.01) less O2- only at 50 microM 1,2-dioctanoyl-sn-glycerol.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
5.
Lefebvre R Theoret C Doré M Girard C Laverty S Vaillancourt D 《The Canadian veterinary journal. La revue veterinaire canadienne》2005,46(11):1029-1033
An 8-year-old Arabian mare was admitted for a large ovarian anovulatory follicle. A clinical diagnosis of ovarian tumor and endometritis was established. Histological examinations revealed an ovarian teratoma and a grade II endometritis. Three months after unilateral ovariectomy, the mare was confirmed pregnant and eventually gave birth uneventfully. 相似文献
6.
Cyclooxygenase (COX)-2 is involved in several physiologic and pathologic processes. COX-2 is overexpressed in human and canine prostate cancer, but little is known about COX-2 inducers in the prostate. Our objective was to investigate the effect of sex steroid hormones on COX-2 expression in the canine prostate in vivo. COX-2 expression was evaluated by immunohistochemistry in intact and castrated dogs treated with exogenous androgen or estrogen. Results showed that no COX-2 staining was observed in prostates of untreated or androgen-treated castrated or intact dogs. However, treatment of intact and castrated dogs with estrogen resulted in squamous metaplasia with intense COX-2 expression observed in both squamous epithelial cells and in cells of acini without metaplasia. This is the first report to demonstrate the induction of COX-2 by estrogen in the prostate in vivo. 相似文献
7.
Tomato fruits at the green mature stage were inoculated with a mixed sporangial suspension of A1 and A2 isolates ofPhytophthora infestans. Other fruits were inoculated with either A1 or A2 sporangia. Seeds were extracted from the blighted fruits and sown in soil or on agar media to test for the transmission of
late blight to the emerging seedlings. Only 23 (0.09%) of approximately 25,000 seedlings developed symptoms. All blighted
seedlings originated from fruits inoculated with mixed A1 + A2 sporangia. Isolates ofP. infestans recovered from the emerging blighted seedlings were seemingly of oosporic origin, as they differed phenotypically (mating
type, virulence, sensitivity to metalaxyl) from the parent isolates used to inoculate the fruits. The results suggest that
transmission ofP. infestans might occur by seeds extracted from fruits carrying oospores and less probably by seeds extracted from fruits having no oospores.
http://www.phytoparasitica.org posting April 30, 2004. 相似文献
8.
Véronique Bernier Gosselin David Francoz Marie Babkine André Desrochers Sylvain Nichols Elizabeth Doré Christian Bédard Joane Parent Julie-Hélène Fairbrother Gilles Fecteau 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(9):957-962
Epidemiological data, clinical findings, laboratory data, medical imaging, and outcomes were reviewed in 29 dairy calves with otitis media/interna. Age at admission ranged from 1 to 24 wk. The majority of calves were referred during winter. Clinical signs included drooping ear, ptosis, head tilt, abnormal nystagmus, strabismus, dysphagia, regurgitation, stiff neck, opisthotonos, facial hyperesthesia, and purulent aural discharge. Intranasal endoscopic examination of 5 animals revealed nasopharyngeal collapse in 4. Cerebrospinal fluid (CSF) was abnormal in all of 7 cases. Mycoplasma bovis was cultured from all but 1 case with external ear or tympanic bullae samples (n = 12), and Mycoplasma arginini was cultured from the remaining ear sample. Radiographs of the tympanic bullae were performed in 24 calves, tomodensitometry (CT) in 3 calves and ultrasound in 4 calves. According to medical imaging techniques or necropsy, 69% of the cases were classified as chronic. Mean duration of treatment was 23.3 d. The rate of clinical recovery was 75%. 相似文献
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